Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   RLE12_RS30050 Genome accession   NZ_CP134695
Coordinates   6397941..6398564 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain TJM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6392941..6403564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RLE12_RS30030 aroB 6393536..6394642 (-) 1107 WP_004352494.1 3-dehydroquinate synthase -
  RLE12_RS30035 aroK 6394692..6395210 (-) 519 WP_003095833.1 shikimate kinase AroK -
  RLE12_RS30040 pilQ 6395222..6397366 (-) 2145 WP_003103267.1 type 4a pilus secretin PilQ Machinery gene
  RLE12_RS30045 comL/pilP 6397420..6397944 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  RLE12_RS30050 pilO 6397941..6398564 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  RLE12_RS30055 pilN 6398561..6399157 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  RLE12_RS30060 pilM 6399157..6400221 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  RLE12_RS30065 - 6400406..6402877 (+) 2472 WP_004352493.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=807472 RLE12_RS30050 WP_003103268.1 6397941..6398564(-) (pilO) [Pseudomonas aeruginosa strain TJM4]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=807472 RLE12_RS30050 WP_003103268.1 6397941..6398564(-) (pilO) [Pseudomonas aeruginosa strain TJM4]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGTGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAATGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401