Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   RLE12_RS30055 Genome accession   NZ_CP134695
Coordinates   6398561..6399157 (-) Length   198 a.a.
NCBI ID   WP_003095839.1    Uniprot ID   G3XD30
Organism   Pseudomonas aeruginosa strain TJM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6393561..6404157
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RLE12_RS30035 aroK 6394692..6395210 (-) 519 WP_003095833.1 shikimate kinase AroK -
  RLE12_RS30040 pilQ 6395222..6397366 (-) 2145 WP_003103267.1 type 4a pilus secretin PilQ Machinery gene
  RLE12_RS30045 comL/pilP 6397420..6397944 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  RLE12_RS30050 pilO 6397941..6398564 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  RLE12_RS30055 pilN 6398561..6399157 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  RLE12_RS30060 pilM 6399157..6400221 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  RLE12_RS30065 - 6400406..6402877 (+) 2472 WP_004352493.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22213.55 Da        Isoelectric Point: 10.0895

>NTDB_id=807473 RLE12_RS30055 WP_003095839.1 6398561..6399157(-) (pilN) [Pseudomonas aeruginosa strain TJM4]
MARINLLPWREELREQRKQQFLVILGGVLVASAALVFLGDQYFTAAIENQNARNDFLRKEIVVLDARIKEISELKSRRQQ
LLERMKIIQDLQGNRPIIGRVFDQLVRTLPDGVYFTDLKMTGKNIAIAGAAESNNRVSNLMRNMDASEWLTAPTLNEVKA
VTQGAVDQANVFQLTVQQTQPGEEDAKAKHGVAQGAKK

Nucleotide


Download         Length: 597 bp        

>NTDB_id=807473 RLE12_RS30055 WP_003095839.1 6398561..6399157(-) (pilN) [Pseudomonas aeruginosa strain TJM4]
ATGGCACGGATCAACCTTCTACCCTGGCGCGAAGAGCTGCGCGAGCAGCGCAAGCAGCAGTTCCTGGTGATTCTTGGCGG
CGTCCTGGTGGCATCGGCTGCGCTGGTATTCCTTGGCGACCAGTACTTCACCGCGGCCATCGAGAACCAGAACGCCCGCA
ACGACTTCCTGCGCAAGGAAATCGTCGTACTCGACGCCCGGATCAAGGAAATCAGCGAACTGAAGTCGCGGCGCCAGCAA
TTGCTCGAGCGGATGAAGATCATCCAGGACCTGCAGGGCAACCGTCCCATCATCGGACGGGTGTTCGACCAGCTCGTGCG
GACCTTGCCCGACGGCGTGTATTTCACCGACCTGAAGATGACCGGCAAGAACATCGCCATCGCCGGCGCGGCCGAGTCCA
ACAACCGCGTTTCCAATCTCATGCGCAACATGGACGCGTCCGAGTGGTTGACCGCCCCGACCCTGAACGAGGTCAAGGCG
GTGACCCAGGGCGCGGTGGACCAGGCCAACGTGTTCCAACTGACCGTGCAGCAGACGCAGCCCGGCGAGGAAGACGCCAA
GGCGAAGCATGGGGTTGCGCAAGGAGCCAAGAAATGA

Domains

Predicted by InterProScan.

Fimbrial assembly protein (PilN) (PilN)

(100-177)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 5EOU

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

41.714

88.384

0.369