Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   P5661_RS13530 Genome accession   NZ_CP120600
Coordinates   2556557..2557174 (-) Length   205 a.a.
NCBI ID   WP_277708803.1    Uniprot ID   -
Organism   Bacillus subtilis strain PRO112     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2551557..2562174
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5661_RS13500 (P5661_13500) rpsT 2551858..2552124 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  P5661_RS13505 (P5661_13505) holA 2552139..2553182 (-) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  P5661_RS13510 (P5661_13510) - 2553222..2553335 (-) 114 WP_122060479.1 hypothetical protein -
  P5661_RS13515 (P5661_13515) yqzM 2553412..2553546 (+) 135 WP_003229983.1 YqzM family protein -
  P5661_RS13520 (P5661_13520) comEC 2553587..2555917 (-) 2331 WP_249848131.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  P5661_RS13525 (P5661_13525) comEB 2555921..2556490 (-) 570 WP_072174291.1 ComE operon protein 2 -
  P5661_RS13530 (P5661_13530) comEA 2556557..2557174 (-) 618 WP_277708803.1 competence protein ComEA Machinery gene
  P5661_RS13535 (P5661_13535) comER 2557258..2558079 (+) 822 WP_021480064.1 late competence protein ComER -
  P5661_RS13540 (P5661_13540) yqeM 2558145..2558888 (-) 744 WP_070547553.1 class I SAM-dependent methyltransferase -
  P5661_RS13545 (P5661_13545) rsfS 2558885..2559241 (-) 357 WP_014480315.1 ribosome silencing factor -
  P5661_RS13550 (P5661_13550) yqeK 2559259..2559819 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  P5661_RS13555 (P5661_13555) nadD 2559809..2560378 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  P5661_RS13560 (P5661_13560) yhbY 2560390..2560680 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  P5661_RS13565 (P5661_13565) aroE 2560674..2561516 (-) 843 WP_070547554.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21838.60 Da        Isoelectric Point: 4.8417

>NTDB_id=806588 P5661_RS13530 WP_277708803.1 2556557..2557174(-) (comEA) [Bacillus subtilis strain PRO112]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVIKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=806588 P5661_RS13530 WP_277708803.1 2556557..2557174(-) (comEA) [Bacillus subtilis strain PRO112]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGATAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGAATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38