NTDB

Overview

Name	comFA	Type	Machinery gene
Locus tag	P5638_RS05715	Genome accession	NZ_CP120599
Coordinates	1159558..1160955 (+)	Length	465 a.a.
NCBI ID	WP_277722972.1	Uniprot ID	-
Organism	Bacillus safensis strain PRO114
Function	ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1154558..1165955

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P5638_RS05695 (P5638_05695)	-	1155561..1156229 (-)	669	WP_012011434.1	YigZ family protein	-
P5638_RS05700 (P5638_05700)	degS	1156450..1157625 (+)	1176	WP_003213166.1	sensor histidine kinase	Regulator
P5638_RS05705 (P5638_05705)	degU	1157690..1158379 (+)	690	WP_008348273.1	two-component system response regulator DegU	Regulator
P5638_RS05710 (P5638_05710)	-	1158595..1159437 (+)	843	WP_277722743.1	DegV family protein	-
P5638_RS05715 (P5638_05715)	comFA	1159558..1160955 (+)	1398	WP_277722972.1	DEAD/DEAH box helicase	Machinery gene
P5638_RS05720 (P5638_05720)	-	1161004..1161297 (+)	294	WP_024426256.1	competence protein ComFB	-
P5638_RS05725 (P5638_05725)	-	1161324..1161812 (+)	489	WP_277722745.1	double zinc ribbon domain-containing protein	-
P5638_RS05730 (P5638_05730)	comFC	1161824..1161979 (+)	156	WP_277722747.1	phosphoribosyltransferase family protein	Machinery gene
P5638_RS05735 (P5638_05735)	-	1162036..1162455 (+)	420	WP_024423621.1	TIGR03826 family flagellar region protein	-
P5638_RS05740 (P5638_05740)	flgM	1162535..1162798 (+)	264	WP_024423620.1	flagellar biosynthesis anti-sigma factor FlgM	-
P5638_RS05745 (P5638_05745)	-	1162817..1163299 (+)	483	WP_034623751.1	flagellar protein FlgN	-
P5638_RS05750 (P5638_05750)	flgK	1163315..1164835 (+)	1521	WP_024423618.1	flagellar hook-associated protein FlgK	-
P5638_RS05755 (P5638_05755)	flgL	1164845..1165753 (+)	909	WP_024423617.1	flagellar hook-associated protein FlgL	-

Sequence

Protein

Download Length: 465 a.a. Molecular weight: 53094.95 Da Isoelectric Point: 9.7681

                                >NTDB_id=806494 P5638_RS05715 WP_277722972.1 1159558..1160955(+) (comFA) [Bacillus safensis strain PRO114]

MNMDKAMELMRHLHSRHLLTVETKCPQSNLDWLEEKGLVNRSPAIERKANGFTCCRCGVSHKRYFAHSPCEVCQKDCVYC

RSCIMMGKAAECGFLYEWTGPQMVETCQAELTWQGELSKGQKRASERMIEAIKNKFDLLVWAVCGAGKTEVLFHGIEYAL

NQGMSVCIATPRTDVVLELEPRLTKAFQGVTITVLYGGSSQRFQITPLVITTTHQLMRYKNAFDVIIVDEVDAFPYSMDE

RLQFAVLKAMRKNRGVRIYLSATPSKKMTREVSRGKLEAIKIPLRFHEQPLPVPTFHWIGHWKKKLKKNQLPPKVMNWMQ

RHIANKRRILLFVPSIATMKKVTKILREQSLNVEGVSADDPDRKQKVQHFRDYVYDVLVTTTILERGVTIPNVQVGVLGS

ESTIFTESALVQISGRVGRHPDYCTGDVFLFHFGLTRSMKQAKKHIAKMNDTAAKEFSEKQCGFN

Nucleotide

Download Length: 1398 bp

                                >NTDB_id=806494 P5638_RS05715 WP_277722972.1 1159558..1160955(+) (comFA) [Bacillus safensis strain PRO114]

TTGAATATGGACAAAGCAATGGAACTAATGCGCCATCTGCATTCACGCCATCTGCTTACTGTTGAAACAAAGTGTCCACA

GTCCAATTTAGATTGGCTAGAGGAGAAGGGCTTAGTCAATCGGTCTCCAGCCATTGAGAGGAAAGCAAATGGTTTTACAT

GCTGCCGGTGCGGTGTATCACACAAGCGGTATTTCGCTCATTCTCCATGTGAGGTGTGTCAAAAGGATTGTGTGTACTGT

AGATCATGCATCATGATGGGAAAAGCAGCAGAATGTGGGTTTCTTTATGAATGGACAGGTCCACAAATGGTAGAAACATG

CCAAGCCGAATTAACATGGCAGGGAGAGCTGTCTAAAGGACAAAAAAGAGCGTCAGAAAGAATGATTGAAGCTATTAAAA

ACAAATTTGATCTACTTGTTTGGGCGGTTTGCGGAGCAGGGAAAACGGAAGTGCTTTTTCACGGAATCGAATATGCGTTA

AATCAAGGAATGAGTGTCTGTATTGCGACACCTAGAACAGATGTAGTGCTTGAACTCGAACCACGGCTTACAAAAGCATT

TCAAGGGGTGACAATTACCGTACTTTACGGAGGCAGTTCTCAAAGGTTTCAAATAACACCGCTTGTGATCACCACAACCC

ACCAGCTGATGAGATACAAAAATGCTTTTGATGTGATCATTGTAGATGAAGTCGATGCCTTCCCTTATTCAATGGATGAG

CGTCTCCAATTTGCTGTTCTAAAGGCGATGAGAAAGAACAGAGGGGTCAGGATTTATTTAAGTGCGACACCCTCTAAAAA

AATGACGAGAGAGGTTTCTCGTGGAAAACTGGAAGCGATAAAAATTCCTTTGCGTTTTCACGAGCAACCATTACCCGTAC

CGACCTTTCATTGGATCGGTCATTGGAAAAAGAAATTAAAAAAGAACCAGCTGCCCCCTAAAGTGATGAATTGGATGCAG

AGACATATTGCAAATAAGAGAAGAATATTACTTTTTGTTCCTTCAATTGCTACCATGAAGAAGGTAACGAAGATTCTTCG

AGAACAATCCTTGAACGTGGAGGGGGTATCTGCCGATGATCCGGATAGAAAACAAAAGGTTCAGCACTTTAGAGATTACG

TATACGATGTACTAGTTACAACCACTATTCTAGAAAGAGGCGTAACGATTCCAAATGTTCAAGTGGGGGTACTAGGTTCG

GAATCTACTATTTTTACAGAGAGTGCACTTGTTCAGATTTCTGGGCGGGTAGGCAGACACCCGGATTATTGTACAGGAGA

TGTTTTCCTTTTTCATTTTGGTTTAACGAGAAGTATGAAACAAGCAAAGAAGCATATCGCAAAAATGAATGATACGGCTG

CAAAAGAGTTTTCTGAAAAACAGTGTGGGTTCAACTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(324-420)

Type III restriction enzyme, res subunit (ResIII)

(116-265)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comFA	Bacillus subtilis subsp. subtilis str. 168	52.193	98.065	0.512
comFA/cflA	Streptococcus pneumoniae Rx1	38.269	94.409	0.361
comFA/cflA	Streptococcus pneumoniae D39	38.269	94.409	0.361
comFA/cflA	Streptococcus pneumoniae R6	38.269	94.409	0.361

Detailed information NTG0806494