Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   P5628_RS18235 Genome accession   NZ_CP120577
Coordinates   3499072..3500463 (-) Length   463 a.a.
NCBI ID   WP_249852325.1    Uniprot ID   -
Organism   Bacillus subtilis strain PRO53     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3494072..3505463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P5628_RS18200 (P5628_18200) flgL 3494244..3495140 (-) 897 WP_015714797.1 flagellar hook-associated protein FlgL -
  P5628_RS18205 (P5628_18205) flgK 3495151..3496674 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  P5628_RS18210 (P5628_18210) flgN 3496693..3497175 (-) 483 WP_014481048.1 flagellar protein FlgN -
  P5628_RS18215 (P5628_18215) flgM 3497191..3497457 (-) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  P5628_RS18220 (P5628_18220) yvyF 3497538..3497957 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  P5628_RS18225 (P5628_18225) comFC 3498030..3498752 (-) 723 WP_032726962.1 comF operon protein ComFC Machinery gene
  P5628_RS18230 (P5628_18230) comFB 3498716..3499012 (-) 297 WP_003227989.1 late competence protein ComFB -
  P5628_RS18235 (P5628_18235) comFA 3499072..3500463 (-) 1392 WP_249852325.1 ATP-dependent helicase ComFA Machinery gene
  P5628_RS18240 (P5628_18240) fakBA 3500569..3501414 (-) 846 WP_003244125.1 DegV family protein -
  P5628_RS18245 (P5628_18245) degU 3501512..3502201 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  P5628_RS18250 (P5628_18250) degS 3502284..3503441 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  P5628_RS18255 (P5628_18255) yvyE 3503658..3504311 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52425.53 Da        Isoelectric Point: 9.8809

>NTDB_id=806333 P5628_RS18235 WP_249852325.1 3499072..3500463(-) (comFA) [Bacillus subtilis strain PRO53]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENAPIWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDAIKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=806333 P5628_RS18235 WP_249852325.1 3499072..3500463(-) (comFA) [Bacillus subtilis strain PRO53]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGATATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGC
ACCAATCTGGAAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGTGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAATAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

97.84

100

0.978