Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   P3T66_RS02655 Genome accession   NZ_CP120512
Coordinates   532721..534058 (+) Length   445 a.a.
NCBI ID   WP_277576167.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain PMC104     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 527721..539058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3T66_RS02640 (P3T66_02640) rny 529158..530723 (+) 1566 WP_011374197.1 ribonuclease Y -
  P3T66_RS02645 (P3T66_02645) - 530879..531967 (+) 1089 WP_035144637.1 glycosyltransferase family 4 protein -
  P3T66_RS02650 (P3T66_02650) - 531994..532659 (-) 666 WP_025016329.1 YigZ family protein -
  P3T66_RS02655 (P3T66_02655) comFA 532721..534058 (+) 1338 WP_277576167.1 DEAD/DEAH box helicase Machinery gene
  P3T66_RS02660 (P3T66_02660) comFC 534301..534729 (+) 429 WP_151384770.1 ComF family protein Machinery gene
  P3T66_RS02665 (P3T66_02665) hpf 534856..535401 (+) 546 WP_011374202.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  P3T66_RS02670 (P3T66_02670) secA 535650..538013 (+) 2364 WP_011374203.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 50413.81 Da        Isoelectric Point: 9.8373

>NTDB_id=805974 P3T66_RS02655 WP_277576167.1 532721..534058(+) (comFA) [Latilactobacillus sakei strain PMC104]
MGQQVIACGRQFTAAQLADTQNNNYSLPQIKRRPAFLRVKHRLVCQRCQQVVPPQTYLPDGRHYCAQCLLFGRLVEGDWL
YTVPECHLFENNIPKLTWEGQLTPHQEQAAQAVVEVVQTQKRHVLTAVTGAGKTEMLFQGILVALQKGQRVCLAAPRVAV
CLELYPRLQAAFATASIMLMHGEQTEPYRYTQLVICTTHQLLKFYHAFDTVIVDEVDAFPFVDNPVLVTAVEQACKPQCA
LLYLTATPTPAIKRAIAAKQMTVSELPLRFHGHLLPEPHRHVAFNWRAQLKKGRLPKRLERDCQTCLKTQQILLFVPQVR
LLQPVANRLSTLLPAVRIETLHANDPDQIKKITAFRAQQIQLMVTTTILERGVTFKNVAVLVLGAEHMVFNEAVLVQIAG
RAGRHKDYAQNPVHFYYQDYTRAIKGACRQIKAQNQRGRRLQSTV

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=805974 P3T66_RS02655 WP_277576167.1 532721..534058(+) (comFA) [Latilactobacillus sakei strain PMC104]
ATGGGACAACAAGTAATTGCCTGTGGTCGCCAGTTTACGGCGGCCCAGTTAGCCGATACGCAAAATAATAACTATAGTTT
ACCACAGATTAAGCGGCGACCTGCGTTTTTACGGGTCAAGCATCGCCTAGTCTGCCAACGTTGTCAGCAAGTGGTGCCGC
CGCAAACCTACTTACCAGATGGGCGCCATTATTGCGCGCAATGTTTGTTATTTGGTCGTTTGGTAGAGGGCGATTGGTTA
TACACGGTACCGGAGTGTCATTTATTCGAAAATAACATACCAAAGTTGACGTGGGAGGGGCAATTAACACCGCATCAGGA
ACAAGCTGCCCAAGCGGTGGTTGAGGTGGTTCAAACACAAAAACGGCACGTATTAACGGCCGTGACCGGCGCAGGGAAGA
CCGAGATGCTTTTTCAAGGAATTTTAGTTGCGCTTCAAAAGGGTCAGCGGGTATGTCTGGCAGCGCCTAGAGTCGCCGTT
TGCTTGGAACTTTATCCGCGCCTGCAAGCTGCCTTTGCAACGGCTAGTATCATGTTGATGCACGGTGAGCAAACTGAGCC
CTATCGCTATACGCAGTTAGTGATTTGCACGACGCATCAGTTGCTGAAGTTTTATCACGCCTTTGACACTGTGATTGTCG
ATGAAGTCGATGCTTTTCCTTTTGTTGATAATCCCGTTTTAGTCACGGCAGTTGAACAAGCCTGCAAGCCCCAATGTGCT
TTATTATATTTAACGGCGACTCCGACCCCCGCTATTAAACGGGCAATTGCTGCTAAACAAATGACGGTTAGCGAATTACC
GCTCCGCTTTCATGGTCATCTATTACCAGAACCACATAGGCACGTCGCTTTTAATTGGCGGGCCCAATTAAAAAAAGGTC
GGTTACCTAAACGACTTGAGCGGGATTGCCAGACTTGTTTAAAAACACAACAGATTTTGTTATTCGTGCCGCAAGTGCGG
TTATTGCAACCAGTTGCCAATCGGTTATCAACCTTATTGCCGGCGGTCAGGATTGAAACATTGCATGCTAACGATCCGGA
TCAGATCAAAAAGATTACGGCGTTTAGAGCACAACAAATTCAGCTAATGGTGACGACCACCATTTTAGAACGGGGCGTTA
CGTTTAAAAACGTTGCAGTTTTGGTCTTAGGCGCGGAACACATGGTGTTTAACGAAGCCGTTTTGGTCCAGATTGCCGGG
CGAGCGGGACGGCATAAAGATTACGCCCAAAATCCGGTTCATTTTTATTATCAAGACTATACGCGGGCGATCAAAGGCGC
TTGTCGGCAAATTAAGGCCCAAAATCAGAGGGGACGGCGATTACAATCAACTGTTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(307-405)

Type III restriction enzyme, res subunit (ResIII)

(101-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Latilactobacillus sakei subsp. sakei 23K

98.427

100

0.984
  comFA/cflA Streptococcus pneumoniae TIGR4

39.211

96.854

0.38
  comFA/cflA Streptococcus pneumoniae D39

38.979

96.854

0.378
  comFA/cflA Streptococcus pneumoniae R6

38.979

96.854

0.378
  comFA/cflA Streptococcus pneumoniae Rx1

38.979

96.854

0.378
  comFA/cflA Streptococcus mitis SK321

38.747

96.854

0.375
  comFA/cflA Streptococcus mitis NCTC 12261

38.515

96.854

0.373
  comFA Bacillus subtilis subsp. subtilis str. 168

39.563

92.584

0.366