Detailed information NTG0805276

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: P3T65_RS20285
• Genome accession: NZ_CP120376
• Coordinates: 4455280..4456509 (-)
• Length: 409 a.a.
• NCBI ID: WP_024767267.1
• Uniprot ID: -
• Organism: Pseudomonas nitroreducens strain L4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4450280..4461509

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P3T65_RS20270 (P3T65_20270)	-	4451584..4452402 (+)	819	WP_024767265.1	helix-turn-helix domain-containing protein	-
P3T65_RS20275 (P3T65_20275)	-	4452519..4453403 (-)	885	WP_024767266.1	DMT family transporter	-
P3T65_RS20280 (P3T65_20280)	-	4453924..4455174 (+)	1251	WP_038803661.1	OprD family porin	-
P3T65_RS20285 (P3T65_20285)	pilU	4455280..4456509 (-)	1230	WP_024767267.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
P3T65_RS20290 (P3T65_20290)	-	4456696..4456974 (+)	279	WP_024767268.1	peptidylprolyl isomerase	-
P3T65_RS20295 (P3T65_20295)	-	4456987..4457349 (+)	363	WP_084359312.1	MmcQ/YjbR family DNA-binding protein	-
P3T65_RS20300 (P3T65_20300)	-	4457349..4457903 (+)	555	WP_065085248.1	putative glycolipid-binding domain-containing protein	-
P3T65_RS20305 (P3T65_20305)	-	4458040..4459221 (+)	1182	WP_277368466.1	MFS transporter	-
P3T65_RS20310 (P3T65_20310)	-	4459505..4460782 (+)	1278	WP_065085247.1	OprD family porin	-
P3T65_RS20315 (P3T65_20315)	-	4460855..4461136 (-)	282	WP_024764475.1	hypothetical protein	-

Sequence

Protein

• Length: 409 a.a.
• Molecular weight: 44674.02 Da
• Isoelectric Point: 6.1281

>NTDB_id=805276 P3T65_RS20285 WP_024767267.1 4455280..4456509(-) (pilU) [Pseudomonas nitroreducens strain L4]
MDIQAMLKILATQDGSDLYLSTGAPPCAKFNGVLKPLSNEPMKAGDVARIGYGLMDEEQRAEFDRELEMNLAISVPGVGR
FRINLFKQRNEVSIVARNIKLDIPLFDDLKLPDVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PIEYVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTQDGKRRAAVEVLLGTATISDIIKRGDFSSIKEIMDKSRALGMQTFDQAL
FDLAVEGAITEEEAVKNADSANNLRLKLKLYKDNPGSVGSTATPAAAKPAAPAPVAPPPATQPVEASSWGLELALEEIEA
EDAEKPAGQ

Nucleotide

• Length: 1230 bp

>NTDB_id=805276 P3T65_RS20285 WP_024767267.1 4455280..4456509(-) (pilU) [Pseudomonas nitroreducens strain L4]
ATGGATATCCAAGCGATGCTCAAGATCCTGGCCACCCAGGATGGTTCGGATCTTTACCTCTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAACGGGGTGCTCAAACCCCTGTCCAACGAGCCGATGAAGGCAGGCGATGTCGCCCGCATCGGCTATGGGC
TGATGGACGAGGAGCAGCGTGCGGAGTTCGACCGCGAGCTGGAGATGAACCTGGCGATTTCAGTTCCCGGTGTAGGGCGT
TTCCGGATCAACCTGTTCAAACAGCGCAATGAAGTGTCCATCGTGGCGCGCAACATCAAGCTGGACATTCCGCTGTTCGA
CGACTTGAAGCTGCCCGACGTGCTGCTCAAGGTGGTCATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CGGGCAAGTCCACTTCCCTGGCGGCGCTGATCGACCACCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAAGAC
CCCATCGAATACGTGCACCGGCACCGCAAGTCGATCATCAACCAGCGCGAAGTGGGCGTGGACACCCGCAGCTTCCATGC
CGCACTGAAAAACACCCTGCGCCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGTGACCGCGAGACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCGATCTCCACGCTGCACGCGAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCCTTCGTTTCCCAGCG
CCTGGTACGCACCCAGGACGGCAAGCGCCGTGCAGCAGTGGAAGTGCTGCTGGGCACGGCGACCATCAGCGACATCATCA
AGCGTGGCGACTTCAGTTCGATCAAGGAGATCATGGACAAGTCCCGTGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGCGGTGGAAGGGGCGATCACCGAGGAAGAGGCGGTGAAGAACGCCGACTCGGCGAACAACCTGCGGCTGAA
GCTCAAGCTGTACAAGGACAACCCGGGCAGTGTCGGCAGCACCGCCACACCTGCGGCTGCCAAGCCAGCTGCCCCGGCGC
CGGTTGCACCGCCGCCGGCCACCCAGCCTGTCGAGGCTTCGAGTTGGGGGCTGGAACTGGCACTGGAAGAGATCGAGGCG
GAAGACGCCGAGAAGCCGGCCGGGCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (113-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.583	87.286	0.494
pilU	Acinetobacter baylyi ADP1	52.958	86.797	0.46
pilU	Vibrio cholerae strain A1552	49.861	88.264	0.44
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.386	95.599	0.377