Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PVE35_RS09765 Genome accession   NZ_CP120359
Coordinates   2146533..2147735 (+) Length   400 a.a.
NCBI ID   WP_277374507.1    Uniprot ID   -
Organism   Pseudomonas sp. AA-38     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2141533..2152735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVE35_RS09750 - 2141859..2142797 (-) 939 WP_277374505.1 L-threonylcarbamoyladenylate synthase -
  PVE35_RS09755 - 2143971..2145866 (-) 1896 WP_277374506.1 patatin-like phospholipase family protein -
  PVE35_RS09760 - 2146107..2146385 (-) 279 WP_017362973.1 peptidylprolyl isomerase -
  PVE35_RS09765 pilU 2146533..2147735 (+) 1203 WP_277374507.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVE35_RS09770 - 2147732..2148481 (-) 750 WP_268177867.1 transporter substrate-binding domain-containing protein -
  PVE35_RS09775 - 2148645..2150600 (-) 1956 WP_277374508.1 acetoacetate--CoA ligase -
  PVE35_RS09780 - 2150791..2151690 (-) 900 WP_277374509.1 hydroxymethylglutaryl-CoA lyase -

Sequence

Protein


Download         Length: 400 a.a.        Molecular weight: 44179.40 Da        Isoelectric Point: 6.2991

>NTDB_id=805190 PVE35_RS09765 WP_277374507.1 2146533..2147735(+) (pilU) [Pseudomonas sp. AA-38]
MDLHAMLKVLSTQDGSDLYLSTGAPPCAKFNGVLKALSQEPLKSGDVAAIAESIMDGAQREEFERELEMNLAISLPGVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLDTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTADGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQAL
IDLVHEGAIDEEEAVKNADSANNVRLKLKLHRDNPANAKPAPVAAAPAAPAKAAAASEWELKLEEIEQEQPPEDPGRHGI

Nucleotide


Download         Length: 1203 bp        

>NTDB_id=805190 PVE35_RS09765 WP_277374507.1 2146533..2147735(+) (pilU) [Pseudomonas sp. AA-38]
ATGGATCTCCATGCAATGCTCAAGGTGCTGTCCACCCAGGATGGTTCCGACCTGTACCTCTCCACGGGCGCACCGCCTTG
CGCCAAGTTCAATGGCGTGCTCAAGGCGCTCAGCCAGGAGCCCTTGAAGTCCGGCGACGTGGCGGCCATCGCCGAGTCGA
TCATGGACGGTGCGCAGCGCGAGGAATTCGAGCGCGAATTGGAGATGAACCTGGCGATCTCTCTGCCGGGCGTCGGGCGT
TTCCGTATCAACATCTTCAAGCAGCGCAACGAGGTGTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCGCGCTTCGA
GGATCTCAAGCTGCCCGAAGTGCTGCTCGACACGGTGATGGAGAAGCGTGGCCTGGTGCTCTTCGTCGGTGGCACCGGCT
CGGGCAAGTCGACCTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGCGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAAGTTGGGGTGGACACTCGCAGCTTCCATGC
CGCGCTGAAGAACACCCTGCGTCAGGCGCCGGACGTGATCCTGATCGGCGAGATCCGTGACCGCGAAACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCCATTTCCACCCTGCATGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAATTTCTTCCCCGAAGAACGTCGTCCGCAACTGCTCAACGATCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
CCTGGTCAAGACCGCCGATGGCAAGCGTCGCGCCGCCGTGGAGGTGATGCTGGGCACGCCGACGGTGCGCGATCTGATCA
AGCGCAACGAGTTCTCCGAGCTCAAGGAAATCATGGAGAAGTCGAAGAACCTGGGCATGCAGACCTTCGATCAGGCGTTG
ATCGACCTGGTGCACGAAGGTGCCATCGACGAGGAAGAGGCGGTGAAGAATGCCGACTCGGCGAACAACGTGCGCCTCAA
GCTCAAGCTGCACCGCGACAATCCAGCCAATGCCAAGCCGGCGCCTGTCGCCGCTGCACCCGCGGCGCCTGCGAAAGCGG
CAGCAGCCAGTGAGTGGGAGCTGAAGCTGGAGGAGATCGAGCAGGAGCAGCCCCCGGAAGATCCGGGGCGCCACGGCATC
TAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.84

87.75

0.49
  pilU Vibrio cholerae strain A1552

52.408

88.25

0.463
  pilU Acinetobacter baylyi ADP1

51.705

88

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.591

93

0.377