Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   P3U20_RS07060 Genome accession   NZ_CP120146
Coordinates   1387820..1388722 (+) Length   300 a.a.
NCBI ID   WP_323705693.1    Uniprot ID   -
Organism   Mammaliicoccus sciuri strain Dog031     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1382820..1393722
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3U20_RS07020 (P3U20_07020) - 1382904..1383458 (+) 555 WP_135016724.1 5-formyltetrahydrofolate cyclo-ligase -
  P3U20_RS07025 (P3U20_07025) - 1383459..1384907 (+) 1449 WP_058611615.1 rhomboid family protein -
  P3U20_RS07030 (P3U20_07030) - 1384912..1385103 (+) 192 WP_078099923.1 YqgQ family protein -
  P3U20_RS07035 (P3U20_07035) - 1385100..1386071 (+) 972 WP_135016726.1 ROK family glucokinase -
  P3U20_RS07040 (P3U20_07040) - 1386071..1386385 (+) 315 WP_058611614.1 MTH1187 family thiamine-binding protein -
  P3U20_RS07045 (P3U20_07045) - 1386415..1386654 (-) 240 WP_016911885.1 DUF2759 domain-containing protein -
  P3U20_RS07050 (P3U20_07050) - 1386752..1387384 (+) 633 WP_058611613.1 MBL fold metallo-hydrolase -
  P3U20_RS07055 (P3U20_07055) - 1387418..1387666 (-) 249 WP_025904960.1 DUF2626 family protein -
  P3U20_RS07060 (P3U20_07060) comGA 1387820..1388722 (+) 903 WP_323705693.1 competence type IV pilus ATPase ComGA Machinery gene
  P3U20_RS07065 (P3U20_07065) comGB 1388712..1389752 (+) 1041 WP_077152202.1 competence type IV pilus assembly protein ComGB -
  P3U20_RS07070 (P3U20_07070) comGC 1389767..1390084 (+) 318 WP_058611611.1 competence type IV pilus major pilin ComGC Machinery gene
  P3U20_RS07075 (P3U20_07075) - 1390113..1390511 (+) 399 WP_049319406.1 hypothetical protein -
  P3U20_RS07080 (P3U20_07080) - 1390495..1390776 (+) 282 WP_323705694.1 hypothetical protein -
  P3U20_RS07085 (P3U20_07085) - 1390751..1391185 (+) 435 WP_323705695.1 competence type IV pilus minor pilin ComGF -
  P3U20_RS07090 (P3U20_07090) - 1391420..1391908 (+) 489 WP_135016732.1 shikimate kinase -
  P3U20_RS07095 (P3U20_07095) gcvT 1392101..1393189 (+) 1089 WP_058611608.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 300 a.a.        Molecular weight: 34360.30 Da        Isoelectric Point: 6.4258

>NTDB_id=803713 P3U20_RS07060 WP_323705693.1 1387820..1388722(+) (comGA) [Mammaliicoccus sciuri strain Dog031]
MEKLFKSIINDAVNSNASDIHFVPLNDDKVFVQMRINGDNIPYDLDLAFNLYSRLLIYMKFIAHLDVSERNKAQSGQYLF
EHDIHYYLRISTIPISLGIESSSIRITPQHFNHSQVKSEHQSYNVLQNRMYQSEGLFLFTGPTGSGKTTLMYELMYALKR
EKNKHIITIEDPVEQTLNGIVQVSVNEKANITYQQSFKAILRCDPDVIMIGEIRDDITAKQVIQASLSGHLVLSTMHASD
TIGAIHRLVELGVTVEQIKQGISCITNQRLVKKCDEARTLDMSYIFKDDIIKYMENNYET

Nucleotide


Download         Length: 903 bp        

>NTDB_id=803713 P3U20_RS07060 WP_323705693.1 1387820..1388722(+) (comGA) [Mammaliicoccus sciuri strain Dog031]
ATGGAGAAATTATTTAAGTCAATTATTAACGATGCTGTCAATTCTAATGCGAGTGATATTCATTTTGTACCTTTAAACGA
TGATAAAGTCTTTGTCCAAATGAGGATAAATGGCGATAATATACCTTATGATTTAGATTTAGCCTTTAATTTGTATAGTA
GGCTACTTATTTATATGAAATTTATCGCTCATCTAGATGTAAGTGAACGAAATAAAGCACAAAGTGGTCAATATTTATTT
GAACATGACATACATTACTATTTAAGAATTTCCACGATACCTATCAGTTTAGGGATAGAATCTAGTTCCATTAGAATAAC
CCCTCAACATTTTAATCATTCACAAGTGAAATCAGAGCATCAATCATATAACGTTCTACAAAACAGAATGTATCAATCAG
AAGGGCTATTTTTATTTACGGGTCCAACTGGATCAGGTAAGACTACATTAATGTATGAACTTATGTACGCATTAAAGAGA
GAGAAGAATAAACATATTATTACGATTGAAGACCCTGTAGAACAAACGTTAAATGGCATCGTTCAAGTTTCAGTCAATGA
AAAAGCTAACATAACTTATCAACAATCCTTTAAAGCCATTTTAAGATGTGATCCGGACGTTATTATGATTGGAGAGATAA
GAGATGATATTACTGCTAAGCAAGTTATTCAAGCTAGTTTAAGTGGTCATTTAGTACTTTCAACAATGCATGCGAGTGAT
ACAATCGGGGCAATCCACCGACTAGTTGAGCTAGGTGTAACAGTCGAACAAATTAAACAAGGTATTTCATGTATTACGAA
CCAACGTCTTGTGAAGAAATGTGATGAAGCAAGAACATTAGATATGTCCTATATTTTTAAAGATGACATTATTAAATATA
TGGAGAATAATTATGAAACGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

52.143

93.333

0.487
  comGA Staphylococcus aureus N315

52.143

93.333

0.487
  comGA Bacillus subtilis subsp. subtilis str. 168

40.58

92

0.373
  pilB Haemophilus influenzae Rd KW20

34.713

100

0.363