Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RJ642_RS10575 Genome accession   NZ_CP134370
Coordinates   2162188..2163198 (+) Length   336 a.a.
NCBI ID   WP_310995186.1    Uniprot ID   -
Organism   Escherichia coli strain TUM12357     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2157188..2168198
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ642_RS10545 (RJ642_10525) nudB 2158912..2159364 (+) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -
  RJ642_RS10550 (RJ642_10530) yebC 2159393..2160133 (+) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  RJ642_RS10555 (RJ642_10535) ruvC 2160168..2160689 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  RJ642_RS10560 (RJ642_10540) yebB 2160691..2161293 (-) 603 WP_001024932.1 YebB family permuted papain-like enzyme -
  RJ642_RS10565 (RJ642_10545) yobI 2161364..2161429 (+) 66 WP_010723105.1 stress response small protein YobI -
  RJ642_RS10570 (RJ642_10550) ruvA 2161568..2162179 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  RJ642_RS10575 (RJ642_10555) ruvB 2162188..2163198 (+) 1011 WP_310995186.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RJ642_RS10580 (RJ642_10560) znuB 2163345..2164130 (-) 786 WP_000571480.1 zinc ABC transporter permease subunit ZnuB -
  RJ642_RS10585 (RJ642_10565) znuC 2164127..2164882 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  RJ642_RS10590 (RJ642_10570) znuA 2164961..2165893 (+) 933 WP_001300644.1 zinc ABC transporter substrate-binding protein ZnuA -
  RJ642_RS10595 (RJ642_10575) mepM 2165909..2167231 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37204.78 Da        Isoelectric Point: 4.7818

>NTDB_id=803100 RJ642_RS10575 WP_310995186.1 2162188..2163198(+) (ruvB) [Escherichia coli strain TUM12357]
MIEADRLISAGTTLQEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=803100 RJ642_RS10575 WP_310995186.1 2162188..2163198(+) (ruvB) [Escherichia coli strain TUM12357]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCAGGAAGATGTAGCAGATCGCGCCATTCGCCCCAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGACCTGTAGGTCTGGATAACCTGGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)

RuvB AAA lid domain (AAA_lid_4)

(184-257)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

54.167

92.857

0.503
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494