Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RJ646_RS10455 Genome accession   NZ_CP134358
Coordinates   2148526..2149536 (+) Length   336 a.a.
NCBI ID   WP_000568519.1    Uniprot ID   Q32HA1
Organism   Escherichia coli strain TUM13936     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2143526..2154536
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RJ646_RS10425 (RJ646_10425) nudB 2145250..2145702 (+) 453 WP_001300367.1 dihydroneopterin triphosphate diphosphatase -
  RJ646_RS10430 (RJ646_10430) yebC 2145731..2146471 (+) 741 WP_000907248.1 YebC/PmpR family DNA-binding transcriptional regulator -
  RJ646_RS10435 (RJ646_10435) ruvC 2146506..2147027 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  RJ646_RS10440 (RJ646_10440) yebB 2147032..2147631 (-) 600 Protein_2034 YebB family permuted papain-like enzyme -
  RJ646_RS10445 (RJ646_10445) yobI 2147702..2147767 (+) 66 WP_010723105.1 stress response small protein YobI -
  RJ646_RS10450 (RJ646_10450) ruvA 2147906..2148517 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  RJ646_RS10455 (RJ646_10455) ruvB 2148526..2149536 (+) 1011 WP_000568519.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RJ646_RS10460 (RJ646_10460) znuB 2149683..2150468 (-) 786 WP_000571478.1 zinc ABC transporter permease subunit ZnuB -
  RJ646_RS10465 (RJ646_10465) znuC 2150465..2151220 (-) 756 WP_000202996.1 zinc ABC transporter ATP-binding protein ZnuC -
  RJ646_RS10470 (RJ646_10470) znuA 2151299..2152231 (+) 933 WP_032170795.1 zinc ABC transporter substrate-binding protein ZnuA -
  RJ646_RS10475 (RJ646_10475) mepM 2152247..2153569 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37173.77 Da        Isoelectric Point: 4.7818

>NTDB_id=802846 RJ646_RS10455 WP_000568519.1 2148526..2149536(+) (ruvB) [Escherichia coli strain TUM13936]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=802846 RJ646_RS10455 WP_000568519.1 2148526..2149536(+) (ruvB) [Escherichia coli strain TUM13936]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATTTGGCTGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTTTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATCGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGCTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCAGCTATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32HA1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

53.251

96.131

0.512
  ruvB Helicobacter pylori 26695

52.038

94.94

0.494