Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RHT76_RS10870 Genome accession   NZ_CP133919
Coordinates   2254049..2255059 (+) Length   336 a.a.
NCBI ID   WP_000568522.1    Uniprot ID   Q8FGR3
Organism   Escherichia coli strain M21170     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2249049..2260059
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RHT76_RS10840 (RHT76_10840) nudB 2250773..2251225 (+) 453 WP_001308712.1 dihydroneopterin triphosphate diphosphatase -
  RHT76_RS10845 (RHT76_10845) yebC 2251254..2251994 (+) 741 WP_000907234.1 YebC/PmpR family DNA-binding transcriptional regulator -
  RHT76_RS10850 (RHT76_10850) ruvC 2252029..2252550 (+) 522 WP_001295503.1 crossover junction endodeoxyribonuclease RuvC -
  RHT76_RS10855 (RHT76_10855) yebB 2252552..2253154 (-) 603 WP_001520719.1 YebB family permuted papain-like enzyme -
  RHT76_RS10860 (RHT76_10860) yobI 2253225..2253290 (+) 66 WP_072146566.1 stress response small protein YobI -
  RHT76_RS10865 (RHT76_10865) ruvA 2253429..2254040 (+) 612 WP_000580323.1 Holliday junction branch migration protein RuvA -
  RHT76_RS10870 (RHT76_10870) ruvB 2254049..2255059 (+) 1011 WP_000568522.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RHT76_RS10875 (RHT76_10875) znuB 2255209..2255994 (-) 786 WP_001520716.1 zinc ABC transporter permease subunit ZnuB -
  RHT76_RS10880 (RHT76_10880) znuC 2255991..2256746 (-) 756 WP_000202987.1 zinc ABC transporter ATP-binding protein ZnuC -
  RHT76_RS10885 (RHT76_10885) znuA 2256825..2257757 (+) 933 WP_011076428.1 zinc ABC transporter substrate-binding protein ZnuA -
  RHT76_RS10890 (RHT76_10890) mepM 2257773..2259095 (+) 1323 WP_001184045.1 murein DD-endopeptidase MepM -
  RHT76_RS10895 (RHT76_10895) tnpA 2259284..2259742 (+) 459 WP_000526135.1 IS200/IS605-like element IS200C family transposase -

Sequence

Protein


Download         Length: 336 a.a.        Molecular weight: 37171.80 Da        Isoelectric Point: 4.7818

>NTDB_id=801656 RHT76_RS10870 WP_000568522.1 2254049..2255059(+) (ruvB) [Escherichia coli strain M21170]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TVRAWNHFGITPPEMP

Nucleotide


Download         Length: 1011 bp        

>NTDB_id=801656 RHT76_RS10870 WP_000568522.1 2254049..2255059(+) (ruvB) [Escherichia coli strain M21170]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCTAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGGGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACACTGATTGGCGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTACGCGATCGTTTTGGTATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCTAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA
ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG
ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

60.486

97.917

0.592
  ruvB Streptococcus pneumoniae TIGR4

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae R6

58.934

94.94

0.56
  ruvB Streptococcus pneumoniae D39

58.934

94.94

0.56
  ruvB Synechocystis sp. PCC 6803

52.941

96.131

0.509
  ruvB Helicobacter pylori 26695

52.366

94.345

0.494