Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   P3G59_RS13445 Genome accession   NZ_CP119967
Coordinates   3020511..3021620 (+) Length   369 a.a.
NCBI ID   WP_277761937.1    Uniprot ID   -
Organism   Pseudomonas sp. A34-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3015511..3026620
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P3G59_RS13430 - 3016455..3017390 (+) 936 WP_277761935.1 sugar kinase -
  P3G59_RS13435 - 3017533..3017808 (-) 276 WP_007912322.1 peptidylprolyl isomerase -
  P3G59_RS13440 - 3017860..3020397 (-) 2538 WP_277761936.1 PAS domain S-box protein -
  P3G59_RS13445 pilU 3020511..3021620 (+) 1110 WP_277761937.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  P3G59_RS13450 - 3021732..3023687 (-) 1956 WP_277761938.1 acetoacetate--CoA ligase -
  P3G59_RS13455 hbdH 3023844..3024617 (-) 774 WP_277761939.1 3-hydroxybutyrate dehydrogenase -
  P3G59_RS13460 - 3024625..3026016 (-) 1392 WP_277761940.1 GntP family permease -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41038.79 Da        Isoelectric Point: 6.7310

>NTDB_id=801245 P3G59_RS13445 WP_277761937.1 3020511..3021620(+) (pilU) [Pseudomonas sp. A34-9]
MEIDALLSILSNKNGSDLFLSTGAPPSARVDGVLTALSERPFKNGETAAIATSLMDAEQRREFDRDLEMNLAISRTGIGR
FRVNIFKQRNDVSIVIRNVRLDIPRFADLKLPAVLLETVMLKQGLILFVGATDSGKSTSLAALIDHRNRHSSGHIITIED
PIEYIHRHQRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVLSTLHAINANQALDRI
INMFAQERRPQLLQTLGNNLKACVSQRLVRTKDGQRRAAVEILLGTPTISDLVRHNELGDLKAMMEKSAEAGMQTFDQSL
FELVEEGAINEDDALKYSDSANNLKLRIKLHREAVGKDNPSSSDWGLMD

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=801245 P3G59_RS13445 WP_277761937.1 3020511..3021620(+) (pilU) [Pseudomonas sp. A34-9]
ATGGAAATCGATGCACTGTTGTCAATTTTGTCCAATAAAAACGGTTCGGATCTGTTCCTCTCCACGGGAGCACCGCCCAG
TGCGCGTGTCGACGGCGTACTGACGGCGTTGAGCGAGCGGCCTTTCAAGAATGGTGAGACCGCCGCCATCGCCACCTCCC
TGATGGACGCCGAGCAGCGACGGGAGTTCGACCGGGATCTGGAAATGAACCTGGCGATTTCGCGAACGGGCATCGGACGT
TTTCGTGTGAACATTTTCAAACAGCGCAACGATGTCTCGATCGTCATTCGCAATGTCAGACTCGACATTCCGCGTTTCGC
TGATCTGAAACTGCCAGCGGTACTGCTTGAAACCGTCATGCTCAAGCAAGGGCTGATTCTGTTCGTCGGTGCCACCGACT
CCGGCAAATCGACGTCGCTGGCTGCGCTGATCGACCACCGCAATCGCCACAGCAGCGGGCACATCATCACCATCGAGGAT
CCGATCGAGTACATCCATCGCCATCAGCGTTCGATCATCAATCAGCGGGAAGTCGGGGTCGATACGCGCAGCTTCCACGC
CGCCTTGAAAAACACGCTGCGCCAGGCCCCGGATGTCGTGCTGATCGGCGAAATCCGTGACCGCGAAACCATGGAGCATG
CCTTGGCGTTTGCCGATACCGGGCATTTGGTGTTGTCGACGTTGCACGCCATTAATGCCAATCAGGCGCTGGACCGGATC
ATCAACATGTTCGCGCAAGAGCGGCGGCCGCAGCTGTTGCAGACGTTGGGTAATAATCTTAAAGCCTGCGTCTCGCAACG
TCTTGTCAGGACCAAGGATGGGCAGAGACGCGCTGCAGTGGAGATATTGCTGGGTACCCCGACTATCAGTGATCTCGTGC
GCCACAACGAATTGGGCGACCTCAAAGCCATGATGGAAAAATCCGCGGAAGCGGGTATGCAGACGTTTGATCAGTCATTG
TTCGAGCTGGTTGAAGAGGGCGCGATCAATGAGGACGATGCGTTGAAATATTCCGATTCGGCCAATAACTTGAAGTTGAG
GATAAAGCTGCATCGAGAGGCCGTCGGCAAAGATAACCCGTCAAGCAGTGATTGGGGGCTGATGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(57-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.028

97.019

0.534
  pilU Acinetobacter baylyi ADP1

53.09

96.477

0.512
  pilU Vibrio cholerae strain A1552

50

98.645

0.493
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.059

91.328

0.366