NTDB

Overview

Name	ruvB	Type	Machinery gene
Locus tag	RHO05_RS13795	Genome accession	NZ_CP133856
Coordinates	2846631..2847641 (-)	Length	336 a.a.
NCBI ID	WP_000568522.1	Uniprot ID	Q8FGR3
Organism	Escherichia coli strain ES1
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2841631..2852641

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RHO05_RS13775 (RHO05_13775)	mepM	2842595..2843917 (-)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-
RHO05_RS13780 (RHO05_13780)	znuA	2843933..2844865 (-)	933	WP_011076428.1	zinc ABC transporter substrate-binding protein ZnuA	-
RHO05_RS13785 (RHO05_13785)	znuC	2844944..2845699 (+)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
RHO05_RS13790 (RHO05_13790)	znuB	2845696..2846481 (+)	786	WP_000571479.1	zinc ABC transporter permease subunit ZnuB	-
RHO05_RS13795 (RHO05_13795)	ruvB	2846631..2847641 (-)	1011	WP_000568522.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
RHO05_RS13800 (RHO05_13800)	ruvA	2847650..2848261 (-)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
RHO05_RS13805 (RHO05_13805)	yobI	2848400..2848465 (-)	66	WP_010723105.1	stress response small protein YobI	-
RHO05_RS13810 (RHO05_13810)	yebB	2848536..2849138 (+)	603	WP_001024929.1	YebB family permuted papain-like enzyme	-
RHO05_RS13815 (RHO05_13815)	ruvC	2849140..2849661 (-)	522	WP_001551239.1	crossover junction endodeoxyribonuclease RuvC	-
RHO05_RS13820 (RHO05_13820)	yebC	2849696..2850436 (-)	741	WP_000907234.1	YebC/PmpR family DNA-binding transcriptional regulator	-
RHO05_RS13825 (RHO05_13825)	nudB	2850465..2850917 (-)	453	WP_001308712.1	dihydroneopterin triphosphate diphosphatase	-

Sequence

Protein

Download Length: 336 a.a. Molecular weight: 37171.80 Da Isoelectric Point: 4.7818

                                >NTDB_id=801152 RHO05_RS13795 WP_000568522.1 2846631..2847641(-) (ruvB) [Escherichia coli strain ES1]

MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG

VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA

TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG

TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA

TVRAWNHFGITPPEMP

Nucleotide

Download Length: 1011 bp

                                >NTDB_id=801152 RHO05_RS13795 WP_000568522.1 2846631..2847641(-) (ruvB) [Escherichia coli strain ES1]

ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT

ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG

CCCTCGATCATCTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC

GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCCGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA

TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC

AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA

ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC

GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC

GTCGCGCTCGCGGTACGCCACGTATTGCCAACCGTCTGCTGCGTCGAGTGCGTGACTTCGCCGAAGTGAAGCACGATGGC

ACCATCTCGGCTGATATCGCTGCTCAGGCGCTGGATATGCTGAATGTCGATGCCGAAGGTTTCGATTATATGGACCGCAA

ATTGTTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTGGCGGCTGCCATTGGCGAAGAAC

GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGCGGGCGTATGGCG

ACAGTGCGGGCGTGGAACCACTTCGGCATCACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(23-181)

RuvB AAA lid domain (AAA_lid_4)

(184-257)

RuvB C-terminal winged helix domain (WHD_RuvB)

(259-328)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q8FGR3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	52.941	96.131	0.509
ruvB	Helicobacter pylori 26695	52.366	94.345	0.494

Detailed information NTG0801152