Detailed information NTG0800428

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RGU79_RS14365
• Genome accession: NZ_CP133717
• Coordinates: 2974308..2975426 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A086HYC5
• Organism: Acinetobacter baumannii strain Ab1
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2969308..2980426

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RGU79_RS14355 (RGU79_14380)	-	2972432..2973124 (-)	693	WP_086262321.1	YggS family pyridoxal phosphate-dependent enzyme	-
RGU79_RS14360 (RGU79_14385)	pilT	2973251..2974288 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RGU79_RS14365 (RGU79_14390)	pilU	2974308..2975426 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RGU79_RS14370 (RGU79_14395)	fur	2975496..2975933 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RGU79_RS14375 (RGU79_14400)	bamE	2976045..2976443 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RGU79_RS14380 (RGU79_14405)	-	2976498..2976821 (-)	324	WP_000442589.1	RnfH family protein	-
RGU79_RS14385 (RGU79_14410)	-	2976821..2977888 (-)	1068	WP_029777448.1	membrane protein	-
RGU79_RS14390 (RGU79_14415)	-	2978041..2978496 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RGU79_RS14395 (RGU79_14420)	-	2978749..2979597 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=800428 RGU79_RS14365 WP_000347036.1 2974308..2975426(+) (pilU) [Acinetobacter baumannii strain Ab1]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=800428 RGU79_RS14365 WP_000347036.1 2974308..2975426(+) (pilU) [Acinetobacter baumannii strain Ab1]
ATGGATTTTAATGACCTGCTCAACCTCATGGTTGAAAAAAAATCATCCGATCTCTTTATTACAGATGGCGTTGCGCCCTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCAGGCGAAGTAATTGGTCAACTATTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCGGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCACCTGTACTTAAAGATTTATCGATGACCAAACGTGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCTACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGCTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTAAGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ACGCGATTGGCTTTGCTGAAACAGGCCATCTTGTATTAGCTACCATGCACGCTAACAACGCCAACCAAGCGCTCGACCGT
ATTATTCACTTTTTTGAGAGTGACCGTCATAGTCAGCTTTACATGGATTTGTCACTTAACTTAAAGGCTATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCACTTAAACATGCTGACTCACCGAACGATTTACG
TTTAACCATTAAACTTGCAGATGAAGGTCCTGATCAATTATCAGACGGAAAACAGCATTTAACTTTTGACCGCCAGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A086HYC5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392