Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   RFN65_RS07105 Genome accession   NZ_CP133703
Coordinates   1403260..1404264 (+) Length   334 a.a.
NCBI ID   WP_309530194.1    Uniprot ID   -
Organism   Bacillus subtilis strain CP35     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1398260..1409264
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RFN65_RS07075 (RFN65_07075) - 1398489..1398611 (+) 123 WP_003229707.1 hypothetical protein -
  RFN65_RS07080 (RFN65_07080) yrzF 1398657..1399304 (-) 648 WP_015483514.1 phosphotransferase -
  RFN65_RS07085 (RFN65_07085) iolG 1399542..1400567 (+) 1026 WP_309530193.1 inositol 2-dehydrogenase -
  RFN65_RS07090 (RFN65_07090) csbX 1400638..1401945 (+) 1308 WP_015483512.1 MFS transporter -
  RFN65_RS07095 (RFN65_07095) bofC 1401993..1402505 (+) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  RFN65_RS07100 (RFN65_07100) ruvA 1402644..1403249 (+) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  RFN65_RS07105 (RFN65_07105) ruvB 1403260..1404264 (+) 1005 WP_309530194.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  RFN65_RS07110 (RFN65_07110) yrzS 1404257..1404457 (+) 201 WP_003222669.1 DUF2905 domain-containing protein -
  RFN65_RS07115 (RFN65_07115) queA 1404487..1405515 (+) 1029 WP_003229723.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  RFN65_RS07120 (RFN65_07120) tgt 1405542..1406687 (+) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  RFN65_RS07125 (RFN65_07125) yajC 1406725..1406991 (+) 267 WP_004398708.1 preprotein translocase subunit YajC -
  RFN65_RS07130 (RFN65_07130) - 1407048..1407437 (-) 390 WP_004398667.1 TIGR04086 family membrane protein -
  RFN65_RS07135 (RFN65_07135) yrbG 1407635..1408291 (+) 657 WP_100506501.1 DUF421 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37451.78 Da        Isoelectric Point: 5.1631

>NTDB_id=800110 RFN65_RS07105 WP_309530194.1 1403260..1404264(+) (ruvB) [Bacillus subtilis strain CP35]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMARLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=800110 RFN65_RS07105 WP_309530194.1 1403260..1404264(+) (ruvB) [Bacillus subtilis strain CP35]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCCATTGTAGCCAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTCATGGCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT
ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB AAA lid domain (AAA_lid_4)

(182-255)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

99.701

100

0.997
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518