Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PY254_RS05250 Genome accession   NZ_CP119392
Coordinates   1237626..1238684 (+) Length   352 a.a.
NCBI ID   WP_281014426.1    Uniprot ID   -
Organism   Rhodanobacter sp. AS-Z3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1232626..1243684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY254_RS05235 (PY254_05235) - 1232736..1234031 (+) 1296 WP_281014423.1 citrate synthase -
  PY254_RS05240 (PY254_05240) - 1234096..1234767 (-) 672 WP_281014424.1 hypothetical protein -
  PY254_RS05245 (PY254_05245) - 1234825..1237356 (-) 2532 WP_281014425.1 penicillin-binding protein 1A -
  PY254_RS05250 (PY254_05250) pilM 1237626..1238684 (+) 1059 WP_281014426.1 pilus assembly protein PilM Machinery gene
  PY254_RS05255 (PY254_05255) - 1238684..1239385 (+) 702 WP_281014427.1 PilN domain-containing protein -
  PY254_RS05260 (PY254_05260) pilO 1239388..1240056 (+) 669 WP_281014428.1 type 4a pilus biogenesis protein PilO -
  PY254_RS05265 (PY254_05265) - 1240053..1240598 (+) 546 WP_281014429.1 pilus assembly protein PilP -
  PY254_RS05270 (PY254_05270) - 1240618..1242813 (+) 2196 WP_281014430.1 type IV pilus secretin PilQ -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37742.26 Da        Isoelectric Point: 4.6217

>NTDB_id=798786 PY254_RS05250 WP_281014426.1 1237626..1238684(+) (pilM) [Rhodanobacter sp. AS-Z3]
MGLFTPKKPALIGVDISSTAVKLLQLSQAGGRYRVEHYAVEPLPPNAVVEKNIVEVEAVGDAIRRALARSGSKLKHASAA
VAGSAVITRIIPMASGLSEDDLEGQIQVEANQYIPYPIDEVSLDYEVLGPVRDNPDMSNVLLAASRTENVDMRVAALDLG
GLTASVIDVEAFAMENAFAMIADQLNVSREALVAVVDIGATMTTLSVLRNQRTIYSREQVFGGKQLTDEIMRRFGLSYEE
AGRAKRKGGLPESYETEALEPFKESLVQQIGRLLQFFFAGSEHSKVDQVVLAGGCASIEGLGPMLEEQLGVSCVIANPLA
RMSLSPRVQAQSLAQDAPALMIAVGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=798786 PY254_RS05250 WP_281014426.1 1237626..1238684(+) (pilM) [Rhodanobacter sp. AS-Z3]
GTGGGGCTCTTTACACCGAAGAAGCCGGCGCTGATTGGTGTCGATATCAGTTCGACAGCGGTCAAGTTGCTGCAGCTAAG
TCAGGCAGGCGGTCGTTATCGCGTGGAGCACTACGCGGTAGAGCCGCTGCCGCCGAATGCCGTGGTTGAGAAAAACATCG
TCGAGGTCGAGGCGGTGGGTGATGCGATTCGTCGTGCGCTGGCACGCTCGGGCTCCAAGCTCAAGCACGCTTCCGCGGCG
GTGGCAGGCTCAGCCGTGATCACCCGCATCATCCCGATGGCCAGCGGGCTGTCCGAGGATGACCTGGAAGGGCAGATCCA
GGTTGAGGCGAATCAATACATTCCGTATCCGATCGACGAAGTCAGCCTCGATTACGAGGTGCTTGGTCCGGTACGCGACA
ATCCGGACATGAGCAACGTGCTGCTGGCGGCCTCGCGCACCGAGAACGTCGACATGCGGGTTGCTGCGCTGGATCTGGGC
GGGCTGACTGCTTCGGTGATCGACGTGGAGGCGTTCGCGATGGAGAACGCGTTCGCGATGATTGCCGATCAGCTCAACGT
CAGTCGCGAAGCCCTGGTGGCGGTGGTCGACATCGGCGCTACCATGACGACGCTGTCCGTGTTGCGCAACCAGCGCACGA
TCTACTCACGCGAACAGGTATTTGGTGGCAAGCAGCTGACCGACGAAATCATGCGTCGCTTCGGGCTTTCCTATGAGGAA
GCCGGGCGCGCCAAGCGCAAGGGTGGCCTGCCCGAGTCTTACGAGACCGAGGCGCTGGAGCCGTTCAAGGAATCCCTGGT
TCAGCAGATCGGCCGCCTGCTGCAGTTTTTCTTCGCCGGCAGTGAGCACAGCAAGGTCGACCAGGTGGTTCTGGCGGGCG
GCTGTGCGTCGATCGAGGGATTGGGTCCGATGCTGGAAGAGCAGCTCGGCGTGTCCTGCGTTATAGCCAATCCGCTGGCA
CGCATGTCGTTGTCGCCCCGGGTTCAGGCTCAGTCGCTGGCGCAGGACGCCCCCGCATTGATGATTGCGGTAGGCCTCGC
CTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.705

100

0.517
  comM Acinetobacter nosocomialis M2

51.42

100

0.514
  comM Acinetobacter baylyi ADP1

50.857

99.432

0.506
  pilM Legionella pneumophila strain ERS1305867

42.776

100

0.429