Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   RB649_RS09495 Genome accession   NZ_CP133179
Coordinates   1751485..1753131 (+) Length   548 a.a.
NCBI ID   WP_011174199.1    Uniprot ID   Q72GK4
Organism   Thermus thermophilus HB27     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1746485..1758131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RB649_RS09465 (RB649_09465) - 1747451..1747879 (+) 429 WP_011174193.1 type II secretion system protein -
  RB649_RS09470 (RB649_09470) - 1747889..1748341 (+) 453 WP_011174194.1 type II secretion system protein -
  RB649_RS09475 (RB649_09475) - 1748345..1748737 (+) 393 WP_011174195.1 hypothetical protein -
  RB649_RS09480 (RB649_09480) - 1748734..1749474 (+) 741 WP_011174196.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RB649_RS09485 (RB649_09485) - 1749471..1750310 (+) 840 WP_011174197.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  RB649_RS09490 (RB649_09490) - 1750320..1751507 (+) 1188 WP_011174198.1 hypothetical protein -
  RB649_RS09495 (RB649_09495) pilB/pilB1 1751485..1753131 (+) 1647 WP_011174199.1 GspE/PulE family protein Machinery gene
  RB649_RS09500 (RB649_09500) pilC 1753135..1754325 (+) 1191 WP_011174200.1 type II secretion system F family protein Machinery gene
  RB649_RS09505 (RB649_09505) - 1754322..1754789 (+) 468 WP_011174201.1 hypothetical protein -
  RB649_RS09510 (RB649_09510) - 1754757..1755488 (-) 732 WP_011174202.1 pseudouridine synthase -
  RB649_RS09515 (RB649_09515) - 1755485..1755682 (-) 198 WP_041443637.1 hypothetical protein -
  RB649_RS09520 (RB649_09520) - 1755702..1756829 (+) 1128 WP_011227734.1 alanine--tRNA ligase-related protein -
  RB649_RS09525 (RB649_09525) - 1756910..1757581 (+) 672 WP_011174204.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 548 a.a.        Molecular weight: 61218.77 Da        Isoelectric Point: 6.0704

>NTDB_id=798713 RB649_RS09495 WP_011174199.1 1751485..1753131(+) (pilB/pilB1) [Thermus thermophilus HB27]
MARPRLGELLLDLGYITEDQLKAALEEQERTGDLLGQILLRRGYIKEQDLVRALADQQRAPLIHPAQTPLDPQALRLLDP
RFAREKRVLPFKVEGNRLHVAMAHPSDLALLDELRFRTGKEIVPYLAPDREILEVLDRLLAEEARPREEARAREEAEAVD
LTLEAMPAVRLAQALLERAIALSASDLHLDPQETHLQVRARIDGVMQDLERLPKDLEAPLAARYKVLAGMDIAERRRSQD
GHFTFSFEGRRYEVRVASVGTLHGEKLTLRIIYPTGVRLGLTELGMLPEELDRFQKLLKRPYGILFVTGPTGSGKTTTLY
AALEQLYTKEKTFVTIEDPVEFPLEGVVQIPVQPKIGLTFAEALRTVLRLDPDVILVGEVRDAETLDTALRAALTGHLVL
ATLHANDAIAALTRLLEMGAERPLLAATLIGTVAQRLVRRVCPQCARPMPVPEEARLYLGERAPEVEKRGVGCPYCRGTG
YRGRIGVYEVYAPDREALSRFAQGAGEEALRERAQALGHRDLWEVGLLQVRAGMTTTGELLRVLGLWE

Nucleotide


Download         Length: 1647 bp        

>NTDB_id=798713 RB649_RS09495 WP_011174199.1 1751485..1753131(+) (pilB/pilB1) [Thermus thermophilus HB27]
TTGGCCCGGCCCAGGCTCGGTGAACTCCTCCTGGACCTCGGCTACATCACCGAGGACCAGCTGAAGGCCGCCCTGGAGGA
GCAGGAGCGCACCGGGGACCTCCTGGGCCAGATCCTCCTCAGGCGGGGCTACATCAAGGAGCAGGACCTGGTGCGGGCCC
TGGCCGACCAGCAACGGGCCCCCTTGATCCACCCCGCCCAGACCCCCCTGGACCCCCAGGCCCTGCGCCTTTTGGACCCC
AGGTTCGCCCGGGAGAAGCGGGTTCTGCCCTTCAAGGTGGAGGGCAACCGCCTCCACGTGGCCATGGCCCACCCCTCGGA
CCTGGCCCTCTTGGACGAGCTCCGCTTCCGCACCGGGAAGGAGATCGTCCCCTACCTGGCCCCGGACCGGGAGATCCTGG
AGGTCCTGGACCGCCTCCTGGCGGAGGAGGCCCGGCCCCGGGAGGAGGCTCGGGCCCGGGAGGAGGCCGAGGCGGTGGAC
CTCACCCTCGAGGCCATGCCCGCCGTCCGCCTGGCCCAGGCCCTTCTGGAGCGGGCCATCGCCCTCTCGGCCAGCGACCT
CCACCTGGACCCCCAGGAAACCCACCTCCAGGTCCGGGCCCGCATTGACGGGGTGATGCAGGACCTGGAGCGCCTTCCCA
AGGACCTGGAGGCGCCCCTGGCCGCCCGCTACAAGGTCCTCGCGGGCATGGACATCGCCGAAAGGCGCCGTTCCCAGGAC
GGCCACTTCACCTTCTCCTTTGAGGGCAGGCGCTACGAGGTGCGGGTGGCCTCAGTGGGCACGCTCCACGGGGAGAAGCT
CACCCTGCGGATCATCTACCCCACGGGGGTGCGGCTCGGCCTCACCGAGCTCGGCATGCTTCCCGAGGAGCTGGACCGCT
TCCAGAAGCTCCTCAAGAGGCCCTACGGCATCCTCTTCGTCACCGGCCCCACGGGAAGCGGCAAGACCACCACCCTCTAC
GCCGCCTTGGAACAGCTTTACACCAAGGAGAAGACCTTCGTCACCATTGAGGACCCGGTGGAGTTTCCCCTCGAGGGGGT
GGTCCAGATCCCCGTCCAGCCCAAGATCGGCCTCACCTTCGCCGAGGCCTTGCGGACCGTGCTCCGCCTGGACCCCGACG
TGATCCTGGTGGGGGAGGTGCGGGACGCCGAGACCCTGGACACCGCCCTTAGGGCCGCCCTCACGGGGCACCTCGTCCTC
GCCACCCTCCACGCCAACGATGCCATCGCCGCCCTTACCCGGCTTCTGGAGATGGGGGCGGAGAGGCCCCTCCTCGCCGC
CACCCTCATCGGCACCGTGGCCCAGCGCCTGGTGCGCCGGGTCTGCCCCCAGTGCGCCAGGCCCATGCCCGTCCCCGAGG
AGGCCCGGCTCTACTTGGGGGAGCGGGCCCCCGAGGTGGAGAAGCGGGGGGTGGGGTGCCCCTACTGCCGGGGGACGGGC
TACCGGGGCCGGATCGGGGTCTACGAGGTCTACGCCCCGGACCGGGAGGCCCTCTCCCGCTTCGCCCAGGGGGCGGGGGA
GGAGGCCCTAAGGGAGCGGGCCCAGGCCCTGGGGCACCGGGACCTCTGGGAGGTGGGCCTCCTTCAGGTGCGGGCGGGGA
TGACCACCACAGGGGAGCTCCTTCGGGTCCTCGGGCTTTGGGAGTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(175-440)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q72GK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.632

100

0.396
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.294

100

0.385
  pilF Thermus thermophilus HB27

38.018

100

0.385
  pilB Acinetobacter baylyi ADP1

37.255

100

0.381
  pilB Vibrio parahaemolyticus RIMD 2210633

35.106

100

0.361