Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PY372_RS14155 Genome accession   NZ_CP119301
Coordinates   3016487..3017080 (-) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain LC     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3011487..3022080
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PY372_RS14135 (PY372_14305) aroB 3012363..3013463 (-) 1101 WP_323709927.1 3-dehydroquinate synthase -
  PY372_RS14140 (PY372_14310) aroK 3013495..3014013 (-) 519 WP_005381319.1 shikimate kinase AroK -
  PY372_RS14145 (PY372_14315) pilQ 3014202..3015950 (-) 1749 WP_025623689.1 type IV pilus secretin PilQ Machinery gene
  PY372_RS14150 (PY372_14320) pilP 3015982..3016497 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  PY372_RS14155 (PY372_14325) pilO 3016487..3017080 (-) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  PY372_RS14160 (PY372_14330) pilN 3017073..3017651 (-) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  PY372_RS14165 (PY372_14335) pilM 3017635..3018651 (-) 1017 WP_005480475.1 type IV pilus biogenesis protein PilM Machinery gene
  PY372_RS14170 (PY372_14340) - 3018794..3021364 (+) 2571 WP_005496567.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=798408 PY372_RS14155 WP_005480482.1 3016487..3017080(-) (pilO) [Vibrio parahaemolyticus strain LC]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=798408 PY372_RS14155 WP_005480482.1 3016487..3017080(-) (pilO) [Vibrio parahaemolyticus strain LC]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAATTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATTGGTCGTTTTTCACAGGCCATTGCCACTCTGCCACGCATCATC
ACCTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726