Detailed information NTG0797668

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: RBH63_RS16880
• Genome accession: NZ_CP132983
• Coordinates: 3087640..3088650 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli strain ARS-C301
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 3082640..3093650

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RBH63_RS16850	nudB	3084364..3084816 (+)	453	WP_077221229.1	dihydroneopterin triphosphate diphosphatase	-
RBH63_RS16855	yebC	3084845..3085585 (+)	741	WP_000907234.1	YebC/PmpR family DNA-binding transcriptional regulator	-
RBH63_RS16860	ruvC	3085620..3086141 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
RBH63_RS16865	yebB	3086143..3086745 (-)	603	WP_001024930.1	YebB family permuted papain-like enzyme	-
RBH63_RS16870	yobI	3086816..3086881 (+)	66	WP_001386853.1	stress response small protein YobI	-
RBH63_RS16875	ruvA	3087020..3087631 (+)	612	WP_000580323.1	Holliday junction branch migration protein RuvA	-
RBH63_RS16880	ruvB	3087640..3088650 (+)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
RBH63_RS16885	znuB	3088797..3089582 (-)	786	WP_000571465.1	zinc ABC transporter permease subunit ZnuB	-
RBH63_RS16890	znuC	3089579..3090334 (-)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
RBH63_RS16895	znuA	3090413..3091345 (+)	933	WP_001342995.1	zinc ABC transporter substrate-binding protein ZnuA	-
RBH63_RS16900	mepM	3091361..3092683 (+)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=797668 RBH63_RS16880 WP_000568519.1 3087640..3088650(+) (ruvB) [Escherichia coli strain ARS-C301]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=797668 RBH63_RS16880 WP_000568519.1 3087640..3088650(+) (ruvB) [Escherichia coli strain ARS-C301]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATCGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494