Detailed information NTG0797564

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: RAB72_RS14250
• Genome accession: NZ_CP132979
• Coordinates: 2777710..2778720 (-)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli strain ARS-C101
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 2772710..2783720

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RAB72_RS14230	mepM	2773677..2774999 (-)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-
RAB72_RS14235	znuA	2775015..2775947 (-)	933	WP_001342995.1	zinc ABC transporter substrate-binding protein ZnuA	-
RAB72_RS14240	znuC	2776026..2776781 (+)	756	WP_000202996.1	zinc ABC transporter ATP-binding protein ZnuC	-
RAB72_RS14245	znuB	2776778..2777563 (+)	786	WP_000571465.1	zinc ABC transporter permease subunit ZnuB	-
RAB72_RS14250	ruvB	2777710..2778720 (-)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
RAB72_RS14255	ruvA	2778729..2779340 (-)	612	WP_000580328.1	Holliday junction branch migration protein RuvA	-
RAB72_RS14260	yobI	2779479..2779544 (-)	66	WP_001386853.1	stress response small protein YobI	-
RAB72_RS14265	yebB	2779615..2780217 (+)	603	WP_001024917.1	YebB family permuted papain-like enzyme	-
RAB72_RS14270	ruvC	2780219..2780740 (-)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
RAB72_RS14275	yebC	2780775..2781515 (-)	741	WP_000907234.1	YebC/PmpR family DNA-binding transcriptional regulator	-
RAB72_RS14280	nudB	2781544..2781996 (-)	453	WP_077221229.1	dihydroneopterin triphosphate diphosphatase	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=797564 RAB72_RS14250 WP_000568519.1 2777710..2778720(-) (ruvB) [Escherichia coli strain ARS-C101]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=797564 RAB72_RS14250 WP_000568519.1 2777710..2778720(-) (ruvB) [Escherichia coli strain ARS-C101]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTGGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATCTGTTGATTTTCGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGTTGTTTATTGATGAGATCCACCGTCTTTCGCCAGTGGTGGAAGAGGTGTTGTATCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGATCGTTTTGGTATTGTGCAGCGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGCGCGCTGGAAGTTGCTC
GTCGAGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494