Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PYW33_RS07000 Genome accession   NZ_CP118963
Coordinates   1434010..1435206 (+) Length   398 a.a.
NCBI ID   WP_004647070.1    Uniprot ID   -
Organism   Acinetobacter lwoffii strain DSM 2403     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
IS/Tn 1435300..1436064 1434010..1435206 flank 94


Gene organization within MGE regions


Location: 1434010..1436064
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PYW33_RS07000 pilU 1434010..1435206 (+) 1197 WP_004647070.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PYW33_RS07005 - 1435300..1436064 (-) 765 WP_004645258.1 IS5 family transposase -

Sequence

Protein


Download         Length: 398 a.a.        Molecular weight: 45141.72 Da        Isoelectric Point: 6.6224

>NTDB_id=796921 PYW33_RS07000 WP_004647070.1 1434010..1435206(+) (pilU) [Acinetobacter lwoffii strain DSM 2403]
MFTAELLDEAKKFMHHMLTKVVEYGGSDLFITADFPPSIKHQGLMKPFGQQELTAEKTKLFAYSLMNDKQRKEFETELEC
NFAITVPGVSRFRVNVFQQQLNVGMVIRTITAEIPNFQKLKLPESLKHVIMEKRGLVLVVGGTGSGKSTSLAAMIDYRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHSWHNALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
SNNANQTLDRIINFFPEERRNQLLMDLSSNMKAIISQRLIRTQDGKGRRAAIEIMLNTPLMSDLILKGEFHELKAIMAKS
RELGMQTFDQALFDLYNQGEISYEEALRNADSTNELRLQIKLKSNRQDTTVATEATQFTMMQEPKAEEKQAENTSVSE

Nucleotide


Download         Length: 1197 bp        

>NTDB_id=796921 PYW33_RS07000 WP_004647070.1 1434010..1435206(+) (pilU) [Acinetobacter lwoffii strain DSM 2403]
ATGTTCACGGCAGAATTGTTGGACGAAGCAAAAAAATTCATGCATCACATGCTGACTAAGGTGGTGGAATATGGCGGTTC
AGATTTATTTATTACGGCTGATTTTCCACCTAGTATTAAACATCAGGGCTTAATGAAACCTTTTGGACAGCAGGAACTCA
CGGCTGAAAAAACCAAGCTGTTTGCCTACAGCTTGATGAATGATAAACAGCGTAAAGAATTTGAAACTGAACTCGAGTGC
AATTTTGCCATCACCGTGCCAGGTGTTTCCCGTTTCCGGGTCAATGTGTTCCAGCAACAACTCAATGTCGGCATGGTAAT
TCGTACCATTACTGCAGAAATTCCAAACTTCCAGAAACTGAAATTACCGGAATCGCTGAAACATGTGATTATGGAAAAAC
GCGGACTGGTGCTGGTGGTCGGTGGAACGGGTTCAGGTAAATCAACTTCACTCGCGGCGATGATTGACTACCGTAATGAA
AATTCGGCCGGGCATATCATTACCGTAGAAGACCCGGTTGAATATGTGCACAAGCATAAAAAATCCATGATTACTCATCG
TGAAGTCGGAGTGGACTGCCATTCCTGGCATAATGCGCTGAAAAATACCTTGCGTCAGGCACCAGATGTGATTCTGATCG
GCGAAATTCGCGATACTGAAACCATGGAACACGCGATTGCTTTTGCTGAAACTGGACATCTGTGTCTGGGAACGCTGCAC
TCGAACAATGCCAACCAGACTTTGGACCGGATTATCAATTTCTTCCCGGAAGAACGCCGGAACCAGCTATTAATGGATTT
GTCATCCAATATGAAGGCGATCATTTCACAGCGTCTGATTCGTACTCAAGATGGTAAAGGCCGTCGTGCAGCGATTGAAA
TCATGTTAAATACGCCACTGATGTCTGACCTGATTTTGAAAGGTGAATTCCACGAATTGAAAGCGATTATGGCGAAATCG
CGTGAACTGGGCATGCAGACCTTTGACCAGGCCTTATTTGACCTGTATAACCAGGGTGAAATTTCTTATGAAGAAGCCTT
GCGCAATGCCGATTCGACCAATGAACTGCGCTTACAAATCAAGCTGAAAAGTAACCGACAGGACACTACCGTAGCCACAG
AGGCAACCCAATTCACCATGATGCAAGAGCCAAAAGCGGAAGAGAAACAAGCAGAGAATACTTCGGTGTCAGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.494

87.437

0.538
  pilU Acinetobacter baylyi ADP1

56.196

87.186

0.49
  pilU Vibrio cholerae strain A1552

52.394

89.196

0.467
  pilT Pseudomonas aeruginosa PAK

44.172

81.91

0.362