Detailed information NTG0079569

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HIY_RS04140
• Genome accession: NZ_AP022867
• Coordinates: 789786..790478 (+)
• Length: 230 a.a.
• NCBI ID: WP_005656260.1
• Uniprot ID: A0A0H3PEJ7
• Organism: Haemophilus influenzae strain 2018-Y40
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 784786..795478

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HIY_RS04120 (HIY_07950)	ampD	786057..786617 (-)	561	WP_005648974.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
HIY_RS04125 (HIY_07960)	pilA	786732..787181 (+)	450	WP_005656280.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
HIY_RS04130 (HIY_07970)	pilB	787178..788572 (+)	1395	WP_005656265.1	GspE/PulE family protein	Machinery gene
HIY_RS04135 (HIY_07980)	pilC	788569..789789 (+)	1221	WP_005656262.1	type II secretion system F family protein	Machinery gene
HIY_RS04140 (HIY_07990)	pilD	789786..790478 (+)	693	WP_005656260.1	A24 family peptidase	Machinery gene
HIY_RS04145 (HIY_08000)	rho	790532..791794 (-)	1263	WP_005648966.1	transcription termination factor Rho	-
HIY_RS04150 (HIY_08010)	metJ	792042..792359 (+)	318	WP_005631186.1	met regulon transcriptional regulator MetJ	-
HIY_RS04155 (HIY_08020)	cueR	792373..792759 (-)	387	WP_005648963.1	Cu(I)-responsive transcriptional regulator	-
HIY_RS04160 (HIY_08030)	-	792836..793042 (+)	207	WP_005689491.1	heavy-metal-associated domain-containing protein	-
HIY_RS04165 (HIY_08040)	-	793116..793322 (+)	207	WP_005660027.1	heavy-metal-associated domain-containing protein	-
HIY_RS04170 (HIY_08050)	-	793396..793602 (+)	207	WP_005656257.1	heavy-metal-associated domain-containing protein	-
HIY_RS04175 (HIY_08060)	-	793676..793882 (+)	207	WP_005656255.1	heavy-metal-associated domain-containing protein	-
HIY_RS04180 (HIY_08070)	-	793956..794162 (+)	207	WP_005656254.1	heavy-metal-associated domain-containing protein	-

Sequence

Protein

• Length: 230 a.a.
• Molecular weight: 26775.84 Da
• Isoelectric Point: 9.3065

>NTDB_id=79569 HIY_RS04140 WP_005656260.1 789786..790478(+) (pilD) [Haemophilus influenzae strain 2018-Y40]
MIYFTMFLLGGILGIALWFYLSGFITRLQQDIYATYVELFPQNRSPFQPHFVSIQQKKCGHILRYFLSVGVGFIFLQIAF
KDSIFTVWIGLTLIILWTISYLDWHYQLISTTPCLWLLTLGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKCYYR
KEAFGRGDYWLAMALGSFIRLETLPHFLLLASVLGICFSLIHKKKKEFIPFAPFMNLSAVIIYFVKYYGY

Nucleotide

• Length: 693 bp

>NTDB_id=79569 HIY_RS04140 WP_005656260.1 789786..790478(+) (pilD) [Haemophilus influenzae strain 2018-Y40]
ATGATTTACTTCACAATGTTTTTATTAGGCGGCATCTTAGGGATCGCATTGTGGTTCTATCTATCTGGTTTTATTACGCG
TTTACAGCAAGATATTTATGCGACTTACGTTGAATTATTTCCACAAAACCGTTCTCCATTTCAACCGCACTTTGTCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAGTGTTGGAGTAGGATTTATATTTTTACAAATTGCCTTC
AAAGATTCTATTTTTACTGTATGGATCGGACTCACACTTATTATTCTTTGGACAATTAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACGACTCCCTGTTTATGGTTACTTACTCTAGGATTATTTGGAGCAGACAATAACTTTTCATTGCTAACGT
TATCTGAAAGCATAAAAAGTGCGGCTAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATGTTATTACAGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCGTTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTGCTTGGAATATGTTTTTCGCTTATTCATAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (92-199)

3D structure

• AlphaFold DB: A0A0H3PEJ7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Haemophilus influenzae 86-028NP	96.087	100	0.961
pilD	Haemophilus influenzae Rd KW20	94.783	100	0.948