Detailed information NTG0795248

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: Q8X47_RS09345
• Genome accession: NZ_CP132061
• Coordinates: 1976444..1977454 (+)
• Length: 336 a.a.
• NCBI ID: WP_000568519.1
• Uniprot ID: Q32HA1
• Organism: Escherichia coli strain QML220614
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1971444..1982454

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q8X47_RS09315	nudB	1973168..1973620 (+)	453	WP_001300367.1	dihydroneopterin triphosphate diphosphatase	-
Q8X47_RS09320	yebC	1973649..1974389 (+)	741	WP_000907234.1	YebC/PmpR family DNA-binding transcriptional regulator	-
Q8X47_RS09325	ruvC	1974424..1974945 (+)	522	WP_001295503.1	crossover junction endodeoxyribonuclease RuvC	-
Q8X47_RS09330	yebB	1974947..1975549 (-)	603	WP_001024904.1	YebB family permuted papain-like enzyme	-
Q8X47_RS09335	yobI	1975620..1975685 (+)	66	WP_072094247.1	stress response small protein YobI	-
Q8X47_RS09340	ruvA	1975824..1976435 (+)	612	WP_000580332.1	Holliday junction branch migration protein RuvA	-
Q8X47_RS09345	ruvB	1976444..1977454 (+)	1011	WP_000568519.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
Q8X47_RS09350	znuB	1977795..1978580 (-)	786	WP_000571479.1	zinc ABC transporter permease subunit ZnuB	-
Q8X47_RS09355	znuC	1978577..1979332 (-)	756	WP_000202990.1	zinc ABC transporter ATP-binding protein ZnuC	-
Q8X47_RS09360	znuA	1979411..1980343 (+)	933	WP_001300644.1	zinc ABC transporter substrate-binding protein ZnuA	-
Q8X47_RS09365	mepM	1980359..1981681 (+)	1323	WP_001184045.1	murein DD-endopeptidase MepM	-

Sequence

Protein

• Length: 336 a.a.
• Molecular weight: 37173.77 Da
• Isoelectric Point: 4.7818

>NTDB_id=795248 Q8X47_RS09345 WP_000568519.1 1976444..1977454(+) (ruvB) [Escherichia coli strain QML220614]
MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIFIKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMG
VNLRTTSGPVLEKAGDLAAMLTNLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARGTPRIANRLLRRVRDFAEVKHDG
TISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKFFGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMA
TTRAWNHFGITPPEMP

Nucleotide

• Length: 1011 bp

>NTDB_id=795248 Q8X47_RS09345 WP_000568519.1 1976444..1977454(+) (ruvB) [Escherichia coli strain QML220614]
ATGATTGAAGCAGACCGTCTGATTTCTGCCGGTACCACTTTGCCGGAAGATGTAGCAGATCGCGCCATTCGCCCTAAATT
ACTGGAAGAGTATGTTGGTCAGCCGCAGGTTCGTTCACAGATGGAGATTTTCATCAAAGCAGCGAAACTGCGCGGCGATG
CCCTCGATCATTTGTTGATTTTTGGTCCTCCGGGGTTGGGTAAAACTACGCTTGCCAACATTGTCGCCAATGAAATGGGC
GTTAATTTACGCACGACTTCTGGTCCGGTGCTGGAAAAGGCGGGCGATCTGGCAGCGATGCTCACTAACCTTGAACCGCA
TGACGTGCTGTTTATTGATGAGATCCACCGTCTATCGCCAGTTGTTGAAGAAGTGCTGTACCCGGCAATGGAAGACTACC
AACTGGATATCATGATTGGTGAAGGTCCGGCGGCACGCTCCATTAAAATTGATTTGCCGCCGTTTACCCTGATTGGTGCA
ACCACGCGCGCAGGTTCGCTGACATCACCGTTGCGCGACCGTTTTGGGATTGTGCAACGTCTGGAGTTTTATCAGGTGCC
GGATCTGCAATATATCGTCAGTCGCAGCGCACGCTTTATGGGGCTTGAGATGAGTGATGACGGGGCGCTGGAAGTTGCTC
GTCGCGCTCGCGGTACGCCGCGCATTGCCAACCGTCTGCTGCGTCGAGTGCGTGATTTCGCCGAAGTGAAGCACGATGGC
ACCATCTCGGCAGATATCGCTGCTCAGGCGTTGGATATGTTGAATGTCGATGCTGAAGGTTTCGATTATATGGACCGCAA
GTTATTGCTGGCGGTAATCGATAAGTTCTTTGGTGGGCCGGTAGGTCTGGATAACCTTGCGGCAGCCATTGGCGAAGAAC
GTGAAACCATTGAGGATGTGCTGGAACCTTATTTGATTCAGCAAGGCTTTTTGCAGCGTACACCGCGTGGGCGTATGGCG
ACGACGCGGGCGTGGAATCACTTTGGCATAACGCCGCCAGAAATGCCGTAA

Domains

Predicted by InterProScan.

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

3D structure

• AlphaFold DB: Q32HA1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	60.486	97.917	0.592
ruvB	Streptococcus pneumoniae TIGR4	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae R6	58.934	94.94	0.56
ruvB	Streptococcus pneumoniae D39	58.934	94.94	0.56
ruvB	Synechocystis sp. PCC 6803	53.251	96.131	0.512
ruvB	Helicobacter pylori 26695	52.038	94.94	0.494