Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   PW220_RS02270 Genome accession   NZ_CP118734
Coordinates   428183..429475 (+) Length   430 a.a.
NCBI ID   WP_248055881.1    Uniprot ID   A0AA96VWV7
Organism   Streptococcus iners subsp. hyiners strain 29892     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 423183..434475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PW220_RS02225 (PW220_02225) - 423198..423446 (+) 249 WP_248055887.1 hypothetical protein -
  PW220_RS02230 (PW220_02230) - 423443..423859 (+) 417 WP_248055886.1 hypothetical protein -
  PW220_RS02235 (PW220_02235) - 423864..424118 (+) 255 WP_248043855.1 phage holin -
  PW220_RS02240 (PW220_02240) - 424205..424891 (+) 687 WP_248055885.1 CHAP domain-containing protein -
  PW220_RS02245 (PW220_02245) - 424910..425335 (+) 426 WP_248055884.1 hypothetical protein -
  PW220_RS02250 (PW220_02250) - 425344..425664 (+) 321 WP_248044537.1 hypothetical protein -
  PW220_RS02255 (PW220_02255) - 425674..426336 (+) 663 WP_248055883.1 hypothetical protein -
  PW220_RS02260 (PW220_02260) - 426654..427157 (+) 504 WP_248055882.1 PBECR4 domain-containing protein -
  PW220_RS02265 (PW220_02265) - 427494..428126 (-) 633 WP_172082949.1 YigZ family protein -
  PW220_RS02270 (PW220_02270) comFA/cflA 428183..429475 (+) 1293 WP_248055881.1 DEAD/DEAH box helicase Machinery gene
  PW220_RS02275 (PW220_02275) comFC/cflB 429468..430133 (+) 666 WP_248055880.1 ComF family protein Machinery gene
  PW220_RS02280 (PW220_02280) hpf 430210..430752 (+) 543 WP_105117239.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48800.28 Da        Isoelectric Point: 8.8566

>NTDB_id=794680 PW220_RS02270 WP_248055881.1 428183..429475(+) (comFA/cflA) [Streptococcus iners subsp. hyiners strain 29892]
MKELEHYYGRLFTKYQLTPAEREKAEKLFSVTDKNACFRCGTTFEEENKLPNDANYCRACLLLGRVRSDEELYHFPQKDF
PVNSCLTWKGKLTDWQQMISDGLVENVGKRQATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSLPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYRAADNAVKEDGVQVFLTATSTDEL
DRKVKTGKLKRLSLPRRFHGNPLVVPQKVWFSKFDANLKKQKLAPKLAKVIKDQRKTGYPLLIFVPEISKGQEFAKIMEK
TFPEETIGFVSSQTENRLKIVEGFRNQDIAILISTTILERGVTFPKVDVIVVQANHYLYTSSSLVQIAGRVGRSIDRPTG
LLQFFHEGSTRSIEKAIVEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=794680 PW220_RS02270 WP_248055881.1 428183..429475(+) (comFA/cflA) [Streptococcus iners subsp. hyiners strain 29892]
ATGAAAGAATTAGAACATTACTATGGAAGACTATTTACCAAATACCAATTGACCCCAGCAGAGCGAGAAAAAGCAGAGAA
GCTATTCAGTGTCACAGACAAGAATGCCTGCTTTCGTTGTGGTACAACTTTCGAAGAAGAAAACAAACTGCCCAATGATG
CAAACTACTGTCGAGCCTGTTTGCTCCTTGGCAGGGTGCGGTCAGATGAAGAACTCTATCATTTTCCTCAGAAAGACTTC
CCTGTAAACTCTTGCTTAACATGGAAGGGGAAATTAACGGATTGGCAGCAAATGATTTCAGATGGATTGGTTGAGAATGT
AGGGAAAAGACAGGCTACACTGGTTCATGCTGTAACAGGAGCAGGAAAAACAGAGATGATATATCATACGGTTGCTTCTG
TGATTGATAAAGGAGGGGCAGTCTGTCTAGCCAGTCCTCGAATAGACGTCTGTATAGAACTCTATAAGCGCCTGCAAAAT
GATTTCTCCCTTCCGATTAGTTTGCTACACGGTGAGTCAGAACCCTATTTCCGAACGCCGCTTGTTGTAGCAACCACTCA
TCAGTTATTAAAATTCTATCAGGCCTTTGATTTGGTCTTGATTGACGAAGTTGATGCCTTTCCCTACGCTGATAATCCCA
TGCTCTACCGTGCTGCTGACAATGCGGTCAAGGAAGACGGTGTCCAAGTATTTTTGACGGCAACGTCAACAGATGAACTG
GACAGAAAAGTCAAAACAGGTAAATTAAAACGACTCAGCCTACCCAGACGATTTCATGGCAATCCACTGGTTGTTCCGCA
AAAGGTTTGGTTTTCCAAATTTGATGCCAATCTAAAGAAACAGAAACTAGCCCCCAAACTAGCTAAGGTAATCAAAGATC
AGAGAAAAACTGGTTATCCTTTGCTCATATTCGTACCAGAAATTTCCAAGGGTCAGGAGTTTGCCAAGATTATGGAAAAA
ACATTTCCAGAAGAAACTATTGGCTTTGTATCCAGTCAAACTGAAAATCGACTTAAGATAGTAGAAGGTTTTCGCAATCA
AGATATTGCCATCCTCATTTCCACTACCATCTTAGAGCGAGGTGTAACCTTCCCAAAAGTAGATGTTATAGTGGTCCAAG
CCAATCATTACCTCTACACATCGTCTAGTCTTGTACAGATTGCTGGTCGTGTAGGTCGAAGTATAGACCGTCCGACAGGC
TTGCTCCAATTTTTCCATGAGGGGTCCACCCGCTCTATTGAGAAAGCTATTGTAGAAATCAAACAAATGAACAAGGAGGC
TGGCTATGTCTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-393)

Type III restriction enzyme, res subunit (ResIII)

(91-235)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

65.661

100

0.658
  comFA/cflA Streptococcus mitis SK321

64.965

100

0.651
  comFA/cflA Streptococcus pneumoniae D39

65.412

98.837

0.647
  comFA/cflA Streptococcus pneumoniae R6

65.412

98.837

0.647
  comFA/cflA Streptococcus pneumoniae TIGR4

65.412

98.837

0.647
  comFA/cflA Streptococcus pneumoniae Rx1

65.412

98.837

0.647
  comFA Lactococcus lactis subsp. cremoris KW2

52.941

94.884

0.502
  comFA Latilactobacillus sakei subsp. sakei 23K

38.928

99.767

0.388
  comFA Bacillus subtilis subsp. subtilis str. 168

37.831

96.512

0.365