Detailed information NTG0794040

Bioinformatically predicted

Overview

• Name: comEC
• Type: Machinery gene
• Locus tag: PWF74_RS01305
• Genome accession: NZ_CP118627
• Coordinates: 255240..257450 (+)
• Length: 736 a.a.
• NCBI ID: WP_165705974.1
• Uniprot ID: -
• Organism: Lactococcus garvieae strain Pa-2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 250240..262450

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PWF74_RS01285 (PWF74_01285)	-	250384..250980 (+)	597	WP_195917752.1	histidine phosphatase family protein	-
PWF74_RS01290 (PWF74_01290)	-	251615..253171 (+)	1557	WP_004259192.1	ABC-F family ATP-binding cassette domain-containing protein	-
PWF74_RS01295 (PWF74_01295)	-	253337..254512 (+)	1176	WP_004259189.1	SLC13 family permease	-
PWF74_RS01300 (PWF74_01300)	-	254570..255220 (+)	651	WP_019299803.1	helix-hairpin-helix domain-containing protein	-
PWF74_RS01305 (PWF74_01305)	comEC	255240..257450 (+)	2211	WP_165705974.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PWF74_RS01310 (PWF74_01310)	-	257605..258381 (+)	777	WP_274978411.1	alpha/beta hydrolase	-
PWF74_RS01315 (PWF74_01315)	-	258577..258792 (+)	216	WP_003135074.1	F0F1 ATP synthase subunit C	-
PWF74_RS01320 (PWF74_01320)	atpB	258847..259560 (+)	714	WP_004259176.1	F0F1 ATP synthase subunit A	-
PWF74_RS01325 (PWF74_01325)	atpF	259574..260080 (+)	507	WP_004259173.1	F0F1 ATP synthase subunit B	-
PWF74_RS01330 (PWF74_01330)	-	260095..260622 (+)	528	WP_165705973.1	F0F1 ATP synthase subunit delta	-
PWF74_RS01335 (PWF74_01335)	atpA	260688..262196 (+)	1509	WP_004259170.1	F0F1 ATP synthase subunit alpha	-

Sequence

Protein

• Length: 736 a.a.
• Molecular weight: 85300.95 Da
• Isoelectric Point: 9.6034

>NTDB_id=794040 PWF74_RS01305 WP_165705974.1 255240..257450(+) (comEC) [Lactococcus garvieae strain Pa-2]
MKQKFSLIYFVYLLVFVYFLIFSFSWPLLILSLFSFFIAFYRQYYSVLVLLLCFSLFLLMIKKETEVQERKQPEKISKIT
PYMDTLQVNGSRLSFRGEVNGNDFQAFYTLTSEKEKEYFKKLAVKGTFYFEGTLEIPEEQRNFSGFDYRKYLRTQGVYRL
LKIEKIHGFEEKQDFDLRLVRRKAILWAQEHFPSPMSSYMTELLFGWLDKDFEEMGELYTSLGIIHLFALSGMQVNFFIE
LLRNILLRLGLSQKLVFFIQIPFSIFYAFLTGLSLSILRALLQKNIPLKNSSDRFSVTFLLLMVFSPYFLLTPGGQLTML
YSFLITFFSRKFKELKGLKKSLLDSTVLAVGVLPLLIFHFHSFQPLSIILTLLFSLIFDFLLLPGLLFVFLLSLFGTALT
LFNPFFIVLESTVRWVDDLFDQPIVFGKPNLIFLLLLFMLSGLALDFYKKRNWNLSIIIVLFLLFFFVKNPKHPSITMVD
VGQGDSILLQDSWNSQNILIDTGGRLKIRADEQWKEGIYKSNAENTLLPYLRARGVSKIDVLIVTHPDEDHIGDLESVAQ
KVPIKNVYVSASALEKKSFAKKLSRLNSQIHIVQEGDKLPIFNSYLWFLTTGVEGKSDNDNSLVTFGEFYGKKFLFTGDL
EEEGEVRLQEEYPHLEVDVLKVGHHGSKTSSKEAFLEAINPKLALISAGKNNRYKHPNQETLQQFAARGIKIYRTDQQGA
IRLLYKKGSWHIQTVK

Nucleotide

• Length: 2211 bp

>NTDB_id=794040 PWF74_RS01305 WP_165705974.1 255240..257450(+) (comEC) [Lactococcus garvieae strain Pa-2]
ATGAAACAAAAATTTTCGCTTATCTATTTCGTTTATCTCTTAGTCTTTGTTTATTTTCTTATTTTTAGTTTTTCATGGCC
TTTACTTATTCTTAGTCTCTTTAGTTTCTTCATCGCTTTTTATCGTCAATACTATAGTGTGTTAGTTCTTTTACTGTGCT
TTAGTTTATTCTTACTCATGATAAAAAAAGAAACGGAAGTACAAGAAAGAAAGCAACCAGAAAAAATTTCGAAAATTACC
CCTTATATGGATACTCTTCAAGTAAATGGAAGCAGGCTCAGTTTCAGAGGAGAAGTGAACGGCAACGACTTCCAAGCTTT
TTACACCTTGACCTCGGAAAAAGAAAAAGAATACTTCAAAAAACTTGCGGTGAAGGGGACTTTTTACTTTGAAGGCACTT
TGGAGATTCCAGAAGAGCAAAGAAACTTTTCAGGTTTTGATTACAGAAAGTATTTAAGGACTCAAGGGGTCTATCGTCTT
CTTAAAATAGAAAAAATTCATGGCTTTGAAGAAAAACAGGACTTTGATTTACGGCTGGTACGCCGAAAAGCCATTCTTTG
GGCTCAGGAACATTTCCCTTCTCCTATGTCGTCCTATATGACAGAGCTTCTTTTTGGGTGGTTGGATAAAGACTTTGAAG
AAATGGGGGAGCTTTACACTAGCCTTGGTATTATCCATTTGTTTGCGCTGTCAGGCATGCAGGTGAATTTTTTTATTGAA
CTTCTCCGAAATATTTTACTACGGCTTGGCTTGTCACAGAAGTTAGTCTTTTTTATCCAAATTCCCTTTTCGATATTTTA
TGCATTTCTTACAGGCTTATCTCTTTCAATCTTACGCGCGCTTCTTCAAAAAAATATCCCCCTTAAAAATTCAAGCGATA
GATTTTCTGTCACTTTTCTTCTTTTAATGGTGTTCAGTCCTTATTTCCTGCTGACACCAGGAGGACAACTCACGATGCTT
TATTCTTTCTTAATCACATTTTTTTCACGAAAGTTCAAAGAACTAAAAGGATTAAAAAAATCGTTACTTGATTCTACCGT
CCTAGCTGTTGGGGTTCTTCCGCTTTTGATTTTTCACTTCCATAGTTTTCAGCCATTATCGATTATCTTGACTTTATTAT
TTAGTCTGATTTTTGATTTTTTACTTCTGCCAGGTTTACTTTTTGTTTTTCTTCTTTCTTTATTCGGTACTGCTCTCACG
TTATTTAATCCTTTTTTTATTGTTTTAGAATCAACAGTAAGATGGGTGGACGATTTATTTGACCAGCCGATTGTCTTTGG
CAAACCCAACCTTATTTTTTTACTTTTACTTTTTATGCTGAGTGGACTTGCCCTTGATTTTTATAAAAAAAGAAACTGGA
ACTTGAGCATAATAATTGTTCTTTTCTTGCTTTTTTTCTTTGTTAAGAATCCTAAACACCCAAGCATTACAATGGTAGAT
GTGGGACAAGGGGACAGTATTCTTTTACAAGACAGTTGGAACAGCCAAAACATCCTCATCGATACGGGGGGGAGATTAAA
AATAAGAGCTGATGAGCAGTGGAAGGAGGGAATCTATAAGAGCAATGCCGAAAATACACTTCTGCCTTATCTGAGAGCGC
GTGGCGTGAGTAAGATAGATGTTCTGATTGTGACACATCCAGATGAGGATCATATTGGAGACCTGGAGAGTGTAGCTCAG
AAAGTACCAATAAAAAATGTTTATGTGTCAGCTTCTGCATTGGAGAAGAAAAGCTTTGCTAAAAAACTCAGCCGTTTAAA
CAGTCAGATTCACATTGTGCAGGAGGGGGACAAGCTCCCGATCTTTAACAGTTATCTCTGGTTTTTAACGACAGGCGTTG
AAGGGAAAAGCGACAATGATAATTCTTTAGTGACATTTGGTGAATTTTATGGGAAGAAATTTCTTTTTACAGGCGACTTA
GAAGAAGAGGGAGAGGTGCGGCTCCAGGAAGAATATCCCCATTTAGAGGTGGATGTCCTAAAAGTTGGACATCATGGCAG
TAAAACTTCTTCAAAGGAAGCTTTTTTAGAAGCAATCAACCCTAAGCTCGCTCTTATTTCGGCTGGAAAGAATAATCGAT
ATAAACATCCCAATCAAGAAACGCTCCAACAATTCGCTGCACGAGGTATAAAAATTTATCGTACAGACCAGCAAGGGGCA
ATCCGTCTCCTTTATAAAAAAGGCAGTTGGCACATTCAAACAGTAAAGTAG

Domains

Predicted by InterproScan.

• Competence protein (Competence) (203-445)

• Domain of unknown function (DUF4131) (DUF4131) (24-166)

• Metallo-beta-lactamase superfamily (Lactamase_B) (481-689)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC	Lactococcus lactis subsp. cremoris KW2	52.933	99.592	0.527
comEC/celB	Streptococcus mitis SK321	45.114	100	0.458
comEC/celB	Streptococcus mitis NCTC 12261	43.683	100	0.442
comEC/celB	Streptococcus pneumoniae Rx1	43.106	100	0.438
comEC/celB	Streptococcus pneumoniae D39	43.106	100	0.438
comEC/celB	Streptococcus pneumoniae R6	43.106	100	0.438
comEC/celB	Streptococcus pneumoniae TIGR4	43.203	100	0.436