Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PVT68_RS09980 Genome accession   NZ_CP118605
Coordinates   2397088..2398164 (-) Length   358 a.a.
NCBI ID   WP_280317637.1    Uniprot ID   -
Organism   Microbulbifer bruguierae strain H12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2392088..2403164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT68_RS09955 (PVT68_09955) - 2392608..2393129 (-) 522 WP_280322498.1 shikimate kinase -
  PVT68_RS09960 (PVT68_09960) - 2393129..2395354 (-) 2226 WP_280317629.1 type IV pilus secretin PilQ family protein -
  PVT68_RS09965 (PVT68_09965) - 2395356..2395883 (-) 528 WP_280317631.1 pilus assembly protein PilP -
  PVT68_RS09970 (PVT68_09970) - 2395880..2396500 (-) 621 WP_280317633.1 type 4a pilus biogenesis protein PilO -
  PVT68_RS09975 (PVT68_09975) - 2396503..2397084 (-) 582 WP_280317635.1 PilN domain-containing protein -
  PVT68_RS09980 (PVT68_09980) pilM 2397088..2398164 (-) 1077 WP_280317637.1 pilus assembly protein PilM Machinery gene
  PVT68_RS09985 (PVT68_09985) - 2398320..2401010 (+) 2691 WP_280317639.1 penicillin-binding protein 1A -
  PVT68_RS09990 (PVT68_09990) - 2401105..2401590 (-) 486 WP_280317641.1 chemotaxis protein CheW -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 38990.31 Da        Isoelectric Point: 4.3560

>NTDB_id=793912 PVT68_RS09980 WP_280317637.1 2397088..2398164(-) (pilM) [Microbulbifer bruguierae strain H12]
MGMLPFLSKGPKAMLGLDISSTSVKLLELSRNGDKYRVESYASEPLPPNAVVDKNINDVGAIAEAIRKVVRRSKTRLRQA
AVAVSGSAVITKTLDMPADLSDDALEAQIALEADQYIPYPLDEVNLDFEVQGPSERGEGQVEVLLAACRSENVEQRVSAL
NEADLLPGVVDVEAYAIERAYTLMDNQFPAQEQLVVAVIDIGATMSALSVLVDGRTVYTREQLFGGRQLTDEIQRRYGLS
SEEAMLAKRQGGSGLPDDYYPEVLEPFRDAVVQQVTRSLQFFYSSTSYSDVDYILLGGGVAAMDGLAELVGQKLNKPTMV
ANPFRDMSVASRVNRQALAVEAPSLMIAAGLAMREREF

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=793912 PVT68_RS09980 WP_280317637.1 2397088..2398164(-) (pilM) [Microbulbifer bruguierae strain H12]
ATGGGGATGCTCCCTTTTCTCAGTAAGGGTCCTAAAGCCATGCTTGGGCTCGATATTAGCTCCACCTCGGTAAAGTTGCT
CGAACTGAGTCGCAATGGCGACAAATACCGCGTGGAAAGCTATGCAAGTGAGCCGTTGCCCCCGAATGCGGTGGTGGACA
AAAATATCAACGACGTGGGTGCCATTGCCGAAGCCATCCGCAAGGTCGTACGGCGTTCCAAGACTCGCCTGCGCCAGGCT
GCAGTGGCGGTATCCGGGTCCGCGGTAATTACCAAGACCCTGGACATGCCTGCAGATCTCTCGGACGACGCTCTGGAAGC
GCAGATTGCGCTGGAGGCGGACCAATATATCCCCTATCCGCTGGATGAAGTGAATCTGGATTTTGAGGTGCAGGGACCGT
CGGAGCGCGGCGAAGGCCAGGTGGAAGTGCTACTGGCGGCATGCCGCAGCGAGAACGTGGAACAGCGCGTATCCGCTTTG
AACGAAGCGGATCTACTGCCCGGGGTGGTGGATGTGGAAGCCTACGCCATTGAGCGGGCCTATACCCTGATGGACAACCA
GTTCCCGGCTCAGGAGCAGCTGGTGGTCGCGGTGATCGATATCGGTGCCACCATGAGTGCGCTTTCGGTGCTGGTTGACG
GTCGTACCGTATACACCCGCGAACAGCTGTTTGGCGGGCGTCAGCTTACCGATGAAATCCAGCGCCGCTACGGTCTGTCT
TCGGAGGAAGCCATGCTGGCCAAGCGCCAGGGTGGCAGCGGCTTGCCCGACGACTACTACCCCGAAGTGCTGGAGCCTTT
CCGCGATGCGGTAGTGCAACAGGTCACTCGTTCCCTGCAATTCTTTTATTCTTCGACCTCCTACAGCGATGTGGATTACA
TCCTGCTCGGTGGCGGCGTCGCCGCGATGGACGGGCTCGCTGAGCTGGTGGGTCAGAAACTCAACAAGCCCACTATGGTG
GCCAATCCCTTCCGGGATATGAGTGTGGCCTCCCGGGTCAATCGTCAGGCACTGGCGGTGGAAGCGCCGTCACTGATGAT
TGCCGCGGGTCTCGCCATGCGCGAGCGGGAGTTTTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(16-355)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

52.273

98.324

0.514
  comM Acinetobacter nosocomialis M2

52.273

98.324

0.514
  comM Acinetobacter baylyi ADP1

47.443

98.324

0.466
  pilM Legionella pneumophila strain ERS1305867

44.382

99.441

0.441