Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PVT68_RS06170 Genome accession   NZ_CP118605
Coordinates   1444836..1446020 (-) Length   394 a.a.
NCBI ID   WP_280321801.1    Uniprot ID   -
Organism   Microbulbifer bruguierae strain H12     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1436956..1447645 1444836..1446020 within 0


Gene organization within MGE regions


Location: 1436956..1447645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVT68_RS06120 (PVT68_06120) - 1437088..1437459 (+) 372 WP_280321788.1 hypothetical protein -
  PVT68_RS06125 (PVT68_06125) - 1437530..1437721 (+) 192 WP_280321789.1 NYN domain-containing protein -
  PVT68_RS06130 (PVT68_06130) - 1437932..1438795 (+) 864 WP_280321791.1 hypothetical protein -
  PVT68_RS06135 (PVT68_06135) - 1439006..1439899 (+) 894 WP_280321793.1 alpha/beta hydrolase -
  PVT68_RS06140 (PVT68_06140) - 1439970..1440689 (+) 720 WP_280321794.1 NYN domain-containing protein -
  PVT68_RS06145 (PVT68_06145) - 1440798..1441280 (+) 483 WP_280321795.1 YcxB family protein -
  PVT68_RS06150 (PVT68_06150) - 1441314..1442363 (+) 1050 WP_280321796.1 oxidoreductase -
  PVT68_RS06155 (PVT68_06155) - 1442503..1443183 (+) 681 WP_280321797.1 zinc metallopeptidase -
  PVT68_RS06160 (PVT68_06160) - 1443190..1444104 (-) 915 WP_280321798.1 LysR family transcriptional regulator -
  PVT68_RS06165 (PVT68_06165) - 1444264..1444755 (+) 492 WP_280321799.1 DoxX family protein -
  PVT68_RS06170 (PVT68_06170) pilU 1444836..1446020 (-) 1185 WP_280321801.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PVT68_RS06175 (PVT68_06175) - 1446485..1447063 (+) 579 WP_280321803.1 DUF3592 domain-containing protein -
  PVT68_RS06180 (PVT68_06180) - 1447190..1447645 (+) 456 WP_280321805.1 hypothetical protein -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 44014.43 Da        Isoelectric Point: 5.9938

>NTDB_id=793906 PVT68_RS06170 WP_280321801.1 1444836..1446020(-) (pilU) [Microbulbifer bruguierae strain H12]
MDFTGYLRVLAKNDGSDLYLSTGAPPCAKFQGVLKPLGKEPLAPGEIEKIAMEIMDEEQREEFKRELEMNLAMTIPNVGR
FRINIFWQRNQVSIVARNIVTEIPKFDDLKLPPILKDVVMAKRGLVLFVGGTGSGKSTSLAALIDHRNTHSGGHIITIED
PVEYVHRHKKSVINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHAIAFAETGHLAISTLHANNANQALDRI
VNFFPEERRPQLLMSLSQNIRAFVSQRLVPTVDGKRCAAVEILLGTQTINELILKGEFHSIKEMMEKSENMGMQTFDGAL
FKLYVEGRISYEEAIKNADSANNLRLRIKLYEEGKKPKVVESDGEGQEDGAKTGGGLSLSLAAIEEEEEELVHY

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=793906 PVT68_RS06170 WP_280321801.1 1444836..1446020(-) (pilU) [Microbulbifer bruguierae strain H12]
ATGGATTTTACCGGTTATCTCCGTGTACTCGCCAAAAACGATGGCTCTGATCTCTACCTCAGTACCGGGGCGCCGCCCTG
CGCCAAGTTCCAGGGGGTACTGAAACCGCTGGGCAAAGAGCCTCTGGCTCCCGGTGAAATTGAGAAAATCGCCATGGAGA
TCATGGATGAGGAGCAGCGTGAGGAGTTCAAGCGCGAGCTGGAAATGAACCTGGCGATGACCATTCCCAACGTGGGTCGC
TTCCGTATCAATATCTTCTGGCAGCGCAATCAGGTCTCCATTGTTGCGCGAAATATCGTCACCGAAATTCCCAAGTTCGA
TGACCTGAAGCTGCCGCCGATCCTGAAGGACGTGGTGATGGCCAAGCGCGGCCTGGTGTTGTTTGTGGGCGGTACCGGCT
CCGGTAAATCCACCTCCCTGGCGGCACTGATCGATCACCGCAATACCCACAGCGGTGGCCATATCATCACCATCGAGGAT
CCGGTGGAATATGTGCACCGGCACAAGAAGAGCGTGATCAACCAGCGCGAAGTGGGGGTGGATACCCGCAGTTTCCACGC
CGCTCTCAAGAACACCCTGCGTCAGGCACCGGATGTGATCCTGATCGGTGAGATCCGTGATCGCGAGACCATGGAGCACG
CCATCGCCTTTGCCGAGACAGGCCACCTGGCCATCTCCACCTTGCACGCCAACAACGCCAACCAGGCGCTGGACCGCATC
GTGAACTTCTTCCCGGAAGAGCGCCGCCCACAGCTGCTGATGTCGCTGTCGCAGAATATCCGCGCGTTCGTCTCCCAGCG
CCTGGTGCCCACCGTGGATGGCAAGCGCTGTGCCGCGGTGGAGATTCTGCTGGGCACCCAGACCATCAATGAGCTGATCC
TCAAGGGCGAATTCCACTCCATCAAGGAAATGATGGAAAAGTCCGAGAACATGGGGATGCAGACGTTTGACGGCGCCCTG
TTCAAACTCTATGTGGAAGGCCGTATCAGCTACGAAGAAGCGATCAAAAACGCGGACTCTGCCAACAACCTGCGCCTGCG
TATCAAGCTGTACGAAGAGGGCAAGAAGCCCAAGGTGGTAGAGTCGGACGGTGAAGGGCAAGAGGACGGTGCCAAGACCG
GCGGTGGACTTTCACTCTCTCTGGCGGCCATCGAGGAGGAAGAGGAAGAGCTGGTACATTACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(66-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.65

89.848

0.5
  pilU Acinetobacter baylyi ADP1

54.039

91.117

0.492
  pilU Vibrio cholerae strain A1552

49.429

88.832

0.439
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.363

86.041

0.373