Detailed information NTG0793324

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: OPI73_RS02055
• Genome accession: NZ_CP118564
• Coordinates: 438855..440003 (+)
• Length: 382 a.a.
• NCBI ID: WP_232683443.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa isolate PA_HN008
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 433855..445003

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OPI73_RS02030 (OPI73_02030)	-	435006..435302 (-)	297	WP_031628073.1	DUF167 domain-containing protein	-
OPI73_RS02035 (OPI73_02035)	-	435299..435892 (-)	594	WP_003084544.1	YggT family protein	-
OPI73_RS02040 (OPI73_02040)	proC	435903..436724 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
OPI73_RS02045 (OPI73_02045)	-	436736..437428 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
OPI73_RS02050 (OPI73_02050)	pilT	437643..438677 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
OPI73_RS02055 (OPI73_02055)	pilU	438855..440003 (+)	1149	WP_232683443.1	type IV pilus ATPase PilU	Machinery gene
OPI73_RS02060 (OPI73_02060)	czcD	440011..440910 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
OPI73_RS02065 (OPI73_02065)	-	441064..441468 (+)	405	WP_003084560.1	NINE protein	-
OPI73_RS02070 (OPI73_02070)	-	441711..443084 (+)	1374	WP_003160915.1	pyridoxal-phosphate dependent enzyme	-
OPI73_RS02075 (OPI73_02075)	-	443081..444265 (+)	1185	WP_014602386.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42562.83 Da
• Isoelectric Point: 6.7792

>NTDB_id=793324 OPI73_RS02055 WP_232683443.1 438855..440003(+) (pilU) [Pseudomonas aeruginosa isolate PA_HN008]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPTGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=793324 OPI73_RS02055 WP_232683443.1 438855..440003(+) (pilU) [Pseudomonas aeruginosa isolate PA_HN008]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTACCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382