Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PWE35_RS05025 Genome accession   NZ_CP118547
Coordinates   1082817..1083911 (+) Length   364 a.a.
NCBI ID   WP_043033789.1    Uniprot ID   A0A0X3QW38
Organism   Stenotrophomonas maltophilia strain HT2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1077817..1088911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWE35_RS05000 (PWE35_05000) - 1078273..1078875 (-) 603 WP_049397188.1 short chain dehydrogenase -
  PWE35_RS05005 (PWE35_05005) - 1078974..1079849 (+) 876 WP_076739592.1 LysR family transcriptional regulator -
  PWE35_RS05010 (PWE35_05010) proC 1080026..1080847 (-) 822 WP_005408367.1 pyrroline-5-carboxylate reductase -
  PWE35_RS05015 (PWE35_05015) - 1080866..1081543 (-) 678 WP_005412486.1 YggS family pyridoxal phosphate-dependent enzyme -
  PWE35_RS05020 (PWE35_05020) pilT 1081631..1082668 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  PWE35_RS05025 (PWE35_05025) pilU 1082817..1083911 (+) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PWE35_RS05030 (PWE35_05030) - 1084119..1085102 (+) 984 WP_005412488.1 aldo/keto reductase -
  PWE35_RS05035 (PWE35_05035) - 1085150..1086049 (-) 900 WP_043033641.1 LysR family transcriptional regulator -
  PWE35_RS05040 (PWE35_05040) - 1086278..1087192 (-) 915 WP_012479096.1 IS110-like element ISStma7 family transposase -
  PWE35_RS05045 (PWE35_05045) - 1087598..1087810 (+) 213 WP_274850083.1 DUF4287 domain-containing protein -
  PWE35_RS05050 (PWE35_05050) - 1087907..1088356 (+) 450 WP_005408373.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 364 a.a.        Molecular weight: 40569.61 Da        Isoelectric Point: 6.7680

>NTDB_id=793248 PWE35_RS05025 WP_043033789.1 1082817..1083911(+) (pilU) [Stenotrophomonas maltophilia strain HT2]
MAHQRASDLFITAGMPPAMKVNGKISPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGLSGVGRFRVSCFYQR
NQVGMVLRRIETRIPTVEELSLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNQNSTGHIITIEDPIEFVHKHE
GCIITQREVGIDTDSWEAALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAMDRIVNFFPEDRR
NQLLMDLSLNLKGVVAQQLVPSPDGRSRKVAMEILLGTPLVQDYIRDGEIHKLKEVMKDSVQLGMKTFDQSLFELYQAGE
ISYEDALRYADSQNEVRLRIKLSQGGDARTLSQGLDGVEISEIR

Nucleotide


Download         Length: 1095 bp        

>NTDB_id=793248 PWE35_RS05025 WP_043033789.1 1082817..1083911(+) (pilU) [Stenotrophomonas maltophilia strain HT2]
ATGGCGCACCAGCGCGCCTCGGACCTGTTCATCACTGCGGGCATGCCGCCGGCGATGAAGGTCAACGGCAAGATCTCGCC
GATCACGCAGACCCCGCTCACGCCGCAGCAGAGCCGCGACCTGGTCCTCAACGTGATGACCCCGGCGCAGCGCGAGGAAT
TCGAGAAGACCCACGAATGCAACTTCGCCATCGGCCTGTCCGGTGTCGGCCGCTTCCGCGTCAGCTGCTTCTACCAGCGC
AACCAGGTCGGCATGGTGCTGCGTCGCATCGAGACGCGCATTCCGACGGTGGAAGAACTGAGCCTGCCGCCGATCATCAA
GACGCTGGCGATGACCAAGCGCGGCATCATCCTGTTCGTCGGCGCCACCGGCACCGGTAAATCGACGTCGCTGGCAGCGA
TGATCGGTTACCGCAACCAGAACTCGACCGGCCACATCATCACCATCGAAGATCCGATCGAGTTCGTGCACAAGCACGAG
GGCTGCATCATCACCCAGCGCGAAGTCGGCATCGATACCGACAGCTGGGAAGCCGCGCTGAAGAACACCCTGCGCCAGGC
GCCGGACGTGATCATGATCGGTGAGGTGCGTACCCGCGAAGGCATGGACCACGCCATCGCGTTCGCCGAAACCGGCCACC
TGGTGCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATGGACCGCATCGTCAATTTCTTCCCGGAAGACCGCCGC
AACCAGCTGCTGATGGACCTGTCGCTGAATCTCAAGGGCGTGGTCGCGCAGCAGCTGGTGCCGTCGCCCGATGGCCGCTC
GCGCAAGGTGGCGATGGAGATCCTGCTGGGCACGCCGCTGGTGCAGGACTACATCCGCGACGGCGAGATCCACAAGCTGA
AGGAAGTGATGAAGGACTCGGTCCAGCTGGGCATGAAGACCTTCGACCAGAGCCTGTTCGAGCTGTACCAGGCCGGCGAG
ATCAGCTACGAGGACGCGCTGCGTTACGCCGATTCGCAGAACGAAGTGCGCCTGCGCATCAAGCTCAGCCAGGGCGGCGA
CGCACGCACGCTGTCACAGGGCCTGGATGGCGTGGAGATCTCCGAGATCCGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(106-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X3QW38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.715

98.352

0.676
  pilU Acinetobacter baylyi ADP1

64.08

95.604

0.613
  pilU Vibrio cholerae strain A1552

54.467

95.33

0.519
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.899

94.78

0.407
  pilT Acinetobacter baumannii strain A118

40.303

90.659

0.365
  pilT Acinetobacter nosocomialis M2

40.303

90.659

0.365
  pilT Acinetobacter baumannii D1279779

40.303

90.659

0.365
  pilT Pseudomonas aeruginosa PAK

40.615

89.286

0.363