Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PWE35_RS05020 Genome accession   NZ_CP118547
Coordinates   1081631..1082668 (+) Length   345 a.a.
NCBI ID   WP_004147099.1    Uniprot ID   A0AAP7J2J0
Organism   Stenotrophomonas maltophilia strain HT2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1076631..1087668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PWE35_RS04995 (PWE35_04995) soxR 1077766..1078200 (+) 435 WP_274850082.1 redox-sensitive transcriptional activator SoxR -
  PWE35_RS05000 (PWE35_05000) - 1078273..1078875 (-) 603 WP_049397188.1 short chain dehydrogenase -
  PWE35_RS05005 (PWE35_05005) - 1078974..1079849 (+) 876 WP_076739592.1 LysR family transcriptional regulator -
  PWE35_RS05010 (PWE35_05010) proC 1080026..1080847 (-) 822 WP_005408367.1 pyrroline-5-carboxylate reductase -
  PWE35_RS05015 (PWE35_05015) - 1080866..1081543 (-) 678 WP_005412486.1 YggS family pyridoxal phosphate-dependent enzyme -
  PWE35_RS05020 (PWE35_05020) pilT 1081631..1082668 (+) 1038 WP_004147099.1 type IV pilus twitching motility protein PilT Machinery gene
  PWE35_RS05025 (PWE35_05025) pilU 1082817..1083911 (+) 1095 WP_043033789.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PWE35_RS05030 (PWE35_05030) - 1084119..1085102 (+) 984 WP_005412488.1 aldo/keto reductase -
  PWE35_RS05035 (PWE35_05035) - 1085150..1086049 (-) 900 WP_043033641.1 LysR family transcriptional regulator -
  PWE35_RS05040 (PWE35_05040) - 1086278..1087192 (-) 915 WP_012479096.1 IS110-like element ISStma7 family transposase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38658.41 Da        Isoelectric Point: 6.6654

>NTDB_id=793247 PWE35_RS05020 WP_004147099.1 1081631..1082668(+) (pilT) [Stenotrophomonas maltophilia strain HT2]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLACPPLFREVIQQPQGLILVTGPTGSGKSTTLAAMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSLITRNQAREYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=793247 PWE35_RS05020 WP_004147099.1 1081631..1082668(+) (pilT) [Stenotrophomonas maltophilia strain HT2]
ATGGATATCGCCGAACTGCTGGCGTTTTCCGTAAAGAACAAAGCGTCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATCCGCGTCGACGGCGACGTTCGCCGGATCAACATCCCAGCCCTGGACCACAAGCAGGTCCATGCGCTGGTGTACGACA
TCATGTCCGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAAGTGGACTTCTCCTTCGAGATTCCATCGCTGGCGCGC
TTCCGAGTGAACGCGTTCAACCAGAACCGTGGTGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAGGTGCTCACCCTCGA
GGACCTGGCCTGCCCGCCGCTGTTCCGCGAAGTGATCCAGCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCATGGCTTCAATGA
AGCACTGCGCTCGGCGCTGCGTGAGGACCCGGACATCATCCTGGTGGGCGAACTTCGTGACCTGGAAACCATCCGCCTGG
CACTGACCGCGGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGTTGCGCGCGGTCATTTCGCAGGC
GCTGCTGAAGAAGGTTGGCGGCGGTCGTACCGCAGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTACTCGGCCATCCAGACTGGCCAGCAGTACGGCATGATGACCCTGGACCAGCACCTG
CAGGATCTGGTCAAGCGCAGCCTGATCACCCGAAACCAGGCCCGTGAGTACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.651

99.71

0.794
  pilT Acinetobacter baumannii D1279779

79.651

99.71

0.794
  pilT Acinetobacter baumannii strain A118

79.651

99.71

0.794
  pilT Pseudomonas stutzeri DSM 10701

78.488

99.71

0.783
  pilT Acinetobacter baylyi ADP1

77.907

99.71

0.777
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75

99.71

0.748
  pilT Legionella pneumophila strain ERS1305867

75

99.71

0.748
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.821

95.362

0.675
  pilT Vibrio cholerae strain A1552

70.821

95.362

0.675
  pilT Neisseria meningitidis 8013

65.689

98.841

0.649
  pilT Neisseria gonorrhoeae MS11

65.396

98.841

0.646
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

40.413

98.261

0.397
  pilU Acinetobacter baylyi ADP1

39.706

98.551

0.391