Detailed information NTG0793061

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PXH81_RS06295
• Genome accession: NZ_CP118539
• Coordinates: 1366099..1367229 (+)
• Length: 376 a.a.
• NCBI ID: WP_011097971.1
• Uniprot ID: Q87CD3
• Organism: Xylella fastidiosa strain ICMP 15197
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1361099..1372229

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PXH81_RS06275 (PXH81_06275)	-	1361386..1362210 (+)	825	WP_011097969.1	glycosyltransferase family 2 protein	-
PXH81_RS06280 (PXH81_06280)	-	1362844..1363671 (-)	828	WP_012382641.1	glycosyltransferase family 2 protein	-
PXH81_RS06285 (PXH81_06285)	-	1364029..1364241 (-)	213	WP_004087301.1	hypothetical protein	-
PXH81_RS06290 (PXH81_06290)	pilT	1364899..1365933 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
PXH81_RS06295 (PXH81_06295)	pilU	1366099..1367229 (+)	1131	WP_011097971.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PXH81_RS06300 (PXH81_06300)	-	1367922..1368890 (-)	969	WP_004087304.1	YafY family protein	-
PXH81_RS06305 (PXH81_06305)	-	1369739..1370548 (+)	810	WP_004087308.1	YdiY family protein	-
PXH81_RS06310 (PXH81_06310)	hemC	1370602..1371519 (-)	918	WP_004087310.1	hydroxymethylbilane synthase	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42479.73 Da
• Isoelectric Point: 6.5563

>NTDB_id=793061 PXH81_RS06295 WP_011097971.1 1366099..1367229(+) (pilU) [Xylella fastidiosa strain ICMP 15197]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=793061 PXH81_RS06295 WP_011097971.1 1366099..1367229(+) (pilU) [Xylella fastidiosa strain ICMP 15197]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

3D structure

• AlphaFold DB: Q87CD3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	50.843	94.681	0.481
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402