Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   PVK49_RS00675 Genome accession   NZ_CP118306
Coordinates   104991..105425 (+) Length   144 a.a.
NCBI ID   WP_002986542.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain 20154608     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99991..110425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVK49_RS00645 (PVK49_00650) - 101659..102024 (+) 366 WP_002986560.1 DUF1033 family protein -
  PVK49_RS00650 (PVK49_00655) comYA 102117..103055 (+) 939 WP_047235612.1 competence type IV pilus ATPase ComGA Machinery gene
  PVK49_RS00655 (PVK49_00660) comGB 102991..104026 (+) 1036 Protein_80 competence type IV pilus assembly protein ComGB -
  PVK49_RS00660 (PVK49_00665) comYC 104028..104354 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  PVK49_RS00665 (PVK49_00670) comGD 104329..104757 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  PVK49_RS00670 (PVK49_00675) comGE 104714..104998 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  PVK49_RS00675 (PVK49_00680) comYF 104991..105425 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  PVK49_RS00680 (PVK49_00685) comGG 105409..105735 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  PVK49_RS00685 (PVK49_00690) comYH 105833..106786 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  PVK49_RS00690 (PVK49_00695) - 106845..108041 (+) 1197 WP_011284424.1 acetate kinase -
  PVK49_RS00695 (PVK49_00700) - 108228..108536 (+) 309 WP_011284425.1 hypothetical protein -
  PVK49_RS00700 (PVK49_00705) proC 108619..109389 (-) 771 WP_032464773.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16573.20 Da        Isoelectric Point: 10.3584

>NTDB_id=791451 PVK49_RS00675 WP_002986542.1 104991..105425(+) (comYF) [Streptococcus pyogenes strain 20154608]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=791451 PVK49_RS00675 WP_002986542.1 104991..105425(+) (comYF) [Streptococcus pyogenes strain 20154608]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAGACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396