Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYH   Type   Machinery gene
Locus tag   PVK49_RS00685 Genome accession   NZ_CP118306
Coordinates   105833..106786 (+) Length   317 a.a.
NCBI ID   WP_002987790.1    Uniprot ID   A0A5S4TCX5
Organism   Streptococcus pyogenes strain 20154608     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100833..111786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PVK49_RS00645 (PVK49_00650) - 101659..102024 (+) 366 WP_002986560.1 DUF1033 family protein -
  PVK49_RS00650 (PVK49_00655) comYA 102117..103055 (+) 939 WP_047235612.1 competence type IV pilus ATPase ComGA Machinery gene
  PVK49_RS00655 (PVK49_00660) comGB 102991..104026 (+) 1036 Protein_80 competence type IV pilus assembly protein ComGB -
  PVK49_RS00660 (PVK49_00665) comYC 104028..104354 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  PVK49_RS00665 (PVK49_00670) comGD 104329..104757 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  PVK49_RS00670 (PVK49_00675) comGE 104714..104998 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  PVK49_RS00675 (PVK49_00680) comYF 104991..105425 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  PVK49_RS00680 (PVK49_00685) comGG 105409..105735 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  PVK49_RS00685 (PVK49_00690) comYH 105833..106786 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  PVK49_RS00690 (PVK49_00695) - 106845..108041 (+) 1197 WP_011284424.1 acetate kinase -
  PVK49_RS00695 (PVK49_00700) - 108228..108536 (+) 309 WP_011284425.1 hypothetical protein -
  PVK49_RS00700 (PVK49_00705) proC 108619..109389 (-) 771 WP_032464773.1 pyrroline-5-carboxylate reductase -
  PVK49_RS00705 (PVK49_00710) pepA 109437..110504 (-) 1068 WP_032464774.1 glutamyl aminopeptidase -
  PVK49_RS00710 (PVK49_00715) - 110969..111253 (+) 285 WP_002992749.1 DUF4651 domain-containing protein -
  PVK49_RS00715 (PVK49_00720) - 111250..111567 (+) 318 WP_002986515.1 thioredoxin family protein -

Sequence

Protein


Download         Length: 317 a.a.        Molecular weight: 35985.08 Da        Isoelectric Point: 4.7205

>NTDB_id=791452 PVK49_RS00685 WP_002987790.1 105833..106786(+) (comYH) [Streptococcus pyogenes strain 20154608]
MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEGASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQT
EQLQANHQFTPDAIGFILLYLLEQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSLKYLKKDGFAIFLAPVNLLTSPQ
SQLLKQWLKDYAQVVALITLPDSIFGHPSNAKSIIVLQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN

Nucleotide


Download         Length: 954 bp        

>NTDB_id=791452 PVK49_RS00685 WP_002987790.1 105833..106786(+) (comYH) [Streptococcus pyogenes strain 20154608]
ATGACTTTTGAAAAAATTGAAGAAGCTTATCAGCTGCTCTTAGAGAACTGTCAACTAATTGAAAATGATCTAAAAACACA
TATATACGATGCCATTGTTGAACAAAACTCCTTTTATTTAGGGGCTGAGGGAGCTAGTCCTCAAGTTGCTCAAAATAGTG
ATAAACTGAAAGCCTTGTGCCTGACAAAAGAAGAATGGCGTAAGGCCTACCAGTTTCTTTTTATTAAGGCAGCTCAGACT
GAGCAACTCCAAGCCAACCATCAGTTCACACCAGATGCTATTGGCTTCATTCTGCTGTATCTTTTGGAACAATTGAGTGA
TAAAGATAGCTTAGAGGTACTTGAGATTGGAAGTGGAACAGGGAACCTAGCCCAAACCCTTCTCAACAACACGAGCAAGA
GCCTTGATTATGTAGGGATTGAACTTGATGATCTCTTGATTGATCTGTCAGCCAGTATTGCTGAGATAATGGATTCTTCA
GCTCATTTTATTCAAGAAGATGCGGTAAGGCCTCAATTACTAAAAGAAAGTGACATTGTCATCAGTGACTTACCAGTTGG
TTATTATCCTAACGATGATATTGCCAAACGGTACAAGGTGGCTAGTTCAGATAAGCATACCTATGCCCATCATTTATTAA
TGGAACAGTCTTTAAAATACTTGAAAAAAGACGGTTTTGCGATTTTTCTGGCACCAGTCAATTTATTGACGAGCCCTCAG
AGCCAGTTATTGAAACAGTGGTTAAAAGATTATGCTCAGGTGGTGGCCTTGATTACGCTACCAGATTCTATTTTTGGTCA
TCCCTCAAATGCCAAGTCCATTATTGTCTTACAAAAACAAACAGACCACCCAATGGAAACCTTTGTCTATCCAATTCGGG
ATTTGAAGCTTGCAGAGAATATTCATGATTTTATGGAAAATTTCAAAAAATGGAAACTGAGTAATGTCAATTAA

Domains

Predicted by InterproScan.

N-6 DNA Methylase (N6_Mtase)

(68-288)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TCX5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYH Streptococcus mutans UA159

66.139

99.685

0.659
  comYH Streptococcus mutans UA140

65.823

99.685

0.656