Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PotMrB4_RS09995 Genome accession   NZ_AP022642
Coordinates   2139825..2141039 (+) Length   404 a.a.
NCBI ID   WP_172433143.1    Uniprot ID   -
Organism   Metapseudomonas otitidis strain MrB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2134825..2146039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PotMrB4_RS09970 (PtoMrB4_19810) - 2134959..2135348 (-) 390 WP_044409036.1 GtrA family protein -
  PotMrB4_RS09975 (PtoMrB4_19820) - 2135345..2136346 (-) 1002 WP_172433141.1 glycosyltransferase family 2 protein -
  PotMrB4_RS09980 (PtoMrB4_19830) - 2136339..2137832 (-) 1494 WP_172433142.1 glycosyltransferase family 39 protein -
  PotMrB4_RS28370 (PtoMrB4_19840) - 2137980..2139284 (-) 1305 WP_044409045.1 PAS domain-containing methyl-accepting chemotaxis protein -
  PotMrB4_RS09990 (PtoMrB4_19850) - 2139403..2139681 (-) 279 WP_044409048.1 peptidylprolyl isomerase -
  PotMrB4_RS09995 (PtoMrB4_19860) pilU 2139825..2141039 (+) 1215 WP_172433143.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PotMrB4_RS10000 (PtoMrB4_19870) - 2141068..2141802 (-) 735 WP_232060816.1 ABC transporter substrate-binding protein -
  PotMrB4_RS10005 (PtoMrB4_19880) - 2141888..2142613 (-) 726 WP_172433144.1 alpha/beta fold hydrolase -
  PotMrB4_RS10010 (PtoMrB4_19890) - 2142594..2143385 (-) 792 WP_044409054.1 crotonase/enoyl-CoA hydratase family protein -
  PotMrB4_RS10015 (PtoMrB4_19900) - 2143738..2145414 (+) 1677 WP_044409057.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 404 a.a.        Molecular weight: 44520.91 Da        Isoelectric Point: 6.6002

>NTDB_id=79064 PotMrB4_RS09995 WP_172433143.1 2139825..2141039(+) (pilU) [Metapseudomonas otitidis strain MrB4]
MDLPSMLKVLASQDGSDLYLSTGAPPCAKFNGVLKPLSADPLKPGDVARIAESIMDPEQRVEFNHELEMNLAISVPNVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLKVVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTSDGKRRAAVEVLLGTSTISDLIKRGDFSSIKEIMEKSKALGMQTFDQAL
FDLVVEGAISEDEAIKNADSANNLRLKLKLYRDGPAPAPTPAPATAPTPPPTATRTQDAASWGLELKLEELPEETPPEDP
GKHF

Nucleotide


Download         Length: 1215 bp        

>NTDB_id=79064 PotMrB4_RS09995 WP_172433143.1 2139825..2141039(+) (pilU) [Metapseudomonas otitidis strain MrB4]
ATGGATCTCCCTTCGATGCTGAAGGTCCTTGCCTCCCAGGATGGCTCGGACCTCTACCTGTCCACCGGCGCGCCGCCCTG
CGCCAAGTTCAATGGCGTGCTCAAGCCGCTGAGCGCCGACCCGCTCAAGCCGGGGGACGTGGCGCGTATCGCCGAGTCGA
TCATGGACCCGGAGCAACGCGTCGAATTCAACCATGAGCTGGAGATGAACCTGGCGATCTCGGTGCCCAACGTGGGGCGC
TTCCGCATCAACATCTTCAAGCAGCGCAACGAGGTCTCCATCGTCGCGCGCAACATCAAGCTGGACATCCCCCGCTTCGA
GGACCTCAAGCTGCCCGAGGTGCTGCTCAAGGTGGTGATGGAGAAGCGCGGCCTGGTGCTCTTCGTCGGCGGCACCGGCT
CCGGCAAGTCCACCTCCCTGGCGGCACTGATCGACTACCGCAACCGCAACAGCGGCGGGCACATCATCACCATCGAGGAC
CCGGTGGAGTATGTGCACCGGCACAAGAAATCCATCATCAACCAGCGCGAGGTGGGCGTGGACACCCGCAGCTTCCACGC
CGCCCTCAAGAACACCCTGCGCCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGCGACCGCGAAACCATGGAGCACG
CCCTGGCCTTCGCCGACACCGGCCACCTGGCCATTTCCACCCTGCACGCGAACAACGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGGCCGCAGCTGCTCAACGACCTTGGCAACAACCTCAAGGCCTTCGTCTCCCAGCG
CCTGGTACGCACTTCCGATGGCAAGCGTCGCGCGGCGGTGGAGGTGCTGCTGGGCACTTCCACCATCAGCGACCTGATCA
AGCGCGGTGACTTCAGCTCCATCAAGGAGATCATGGAAAAGTCCAAGGCCCTGGGCATGCAGACCTTCGACCAGGCGCTG
TTCGACCTGGTGGTGGAAGGCGCCATCAGCGAGGACGAGGCGATCAAGAACGCCGACTCCGCCAACAACCTGCGCCTCAA
GCTGAAGCTCTACCGCGACGGCCCGGCTCCCGCACCGACGCCCGCTCCCGCCACCGCACCCACCCCGCCGCCGACCGCGA
CCCGTACCCAGGACGCAGCCAGCTGGGGCCTGGAGCTGAAGCTGGAGGAGCTGCCCGAGGAAACGCCGCCGGAAGATCCG
GGCAAGCATTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

56.125

86.881

0.488
  pilU Acinetobacter baylyi ADP1

52.925

88.861

0.47
  pilU Vibrio cholerae strain A1552

52.125

87.376

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.83

93.069

0.361

Multiple sequence alignment