Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   PUW71_RS02195 Genome accession   NZ_CP118053
Coordinates   418973..420274 (+) Length   433 a.a.
NCBI ID   WP_024052084.1    Uniprot ID   A0AAW5TE88
Organism   Streptococcus anginosus strain VSI40     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 413973..425274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUW71_RS02175 (PUW71_02175) - 415912..416328 (+) 417 WP_024052089.1 hypothetical protein -
  PUW71_RS02180 (PUW71_02180) dcm 416485..416930 (+) 446 Protein_387 DNA (cytosine-5-)-methyltransferase -
  PUW71_RS02185 (PUW71_02185) cysK 417252..418181 (-) 930 WP_024052086.1 cysteine synthase A -
  PUW71_RS02190 (PUW71_02190) - 418281..418916 (-) 636 WP_024052085.1 YigZ family protein -
  PUW71_RS02195 (PUW71_02195) comFA/cflA 418973..420274 (+) 1302 WP_024052084.1 DEAD/DEAH box helicase Machinery gene
  PUW71_RS02200 (PUW71_02200) comFC/cflB 420271..420936 (+) 666 WP_024052083.1 ComF family protein Machinery gene
  PUW71_RS02205 (PUW71_02205) raiA 421014..421556 (+) 543 WP_003025754.1 ribosome-associated translation inhibitor RaiA -
  PUW71_RS02210 (PUW71_02210) rsmD 421744..422292 (+) 549 WP_171021853.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  PUW71_RS02215 (PUW71_02215) coaD 422282..422779 (+) 498 WP_021001374.1 pantetheine-phosphate adenylyltransferase -
  PUW71_RS02220 (PUW71_02220) sepM 422760..423815 (+) 1056 WP_024052081.1 SepM family pheromone-processing serine protease Regulator
  PUW71_RS02225 (PUW71_02225) - 423954..424517 (+) 564 WP_024052080.1 YutD-like domain-containing protein -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49050.81 Da        Isoelectric Point: 8.0447

>NTDB_id=789592 PUW71_RS02195 WP_024052084.1 418973..420274(+) (comFA/cflA) [Streptococcus anginosus strain VSI40]
MTELQDCLGRIFTKNQLSPELQLQAQTLTGMVEEKGRLSCNRCGQAIDKEKHQLPIGAYYCRSCLILGRVRSDEDLYYFP
QEEFPKANVLKWQGKLTEFQAKVSQGLVEAVTKRKDSLVHAVTGAGKTEMIYQVVAQVINQGGAVCLASPRIDVCLELYR
RLKVDFTCDISLLHGESEAYSRSPLVIATTHQLIKFYRAFDLLIVDEVDAFPYVDNPMLYHAVHQAVKVEGTKIFLTATS
TDELDKKVAKGELTRLSLPRRFHGNPLIVPQKIWLADFQKNLGQKKLVPKLRQFIQKQRKTGFPLLIFASEIRKGQELAE
ILQSTFPNEEVDFVASTTENRLEIVEKFRQKEITILVTTTILERGVTFPCVDVFVVEANHRLFSRSALVQIAGRVGRSME
RPTGELIFFHDGTTMAIEKAIKEIQEMNQEAGL

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=789592 PUW71_RS02195 WP_024052084.1 418973..420274(+) (comFA/cflA) [Streptococcus anginosus strain VSI40]
ATGACAGAATTACAAGATTGTTTAGGTCGCATTTTTACAAAAAATCAACTGTCACCAGAATTGCAATTGCAAGCACAAAC
CTTAACTGGAATGGTAGAAGAAAAAGGGAGATTAAGCTGTAATCGCTGTGGACAAGCCATTGACAAAGAAAAACACCAAC
TGCCAATCGGTGCTTATTATTGTAGGTCTTGCTTGATCTTAGGAAGAGTTAGGAGTGATGAAGATCTCTACTATTTTCCA
CAGGAAGAATTTCCCAAAGCGAATGTCTTGAAATGGCAAGGAAAGTTGACAGAATTTCAAGCCAAGGTTTCTCAAGGACT
TGTAGAGGCGGTTACCAAACGCAAAGATAGCTTGGTTCACGCAGTCACGGGAGCCGGAAAGACGGAAATGATCTATCAAG
TGGTGGCACAAGTCATCAATCAGGGCGGAGCAGTCTGCTTGGCTAGCCCCAGAATTGATGTCTGCTTAGAACTTTATCGC
AGACTGAAAGTGGATTTTACTTGTGATATTTCACTCCTGCACGGTGAATCAGAAGCATATTCTCGCAGTCCTCTCGTGAT
TGCCACCACACATCAGCTTATCAAATTTTACCGAGCATTTGATCTTCTTATTGTTGATGAGGTAGATGCCTTTCCTTATG
TGGACAATCCGATGCTTTATCATGCAGTTCATCAGGCAGTCAAAGTAGAGGGGACGAAGATTTTCTTAACAGCAACTTCC
ACAGATGAGCTGGATAAAAAAGTGGCTAAAGGAGAATTAACTCGTTTGAGTCTACCCAGACGTTTTCATGGCAATCCTTT
GATTGTTCCGCAAAAAATTTGGTTGGCGGATTTTCAAAAAAATCTTGGTCAAAAGAAGCTAGTTCCCAAGTTAAGGCAGT
TTATTCAAAAGCAGAGAAAAACAGGATTTCCACTTCTTATTTTTGCTTCCGAGATCAGAAAAGGGCAGGAGCTGGCAGAG
ATTCTTCAAAGCACTTTTCCTAATGAAGAAGTTGATTTTGTAGCCTCGACGACTGAAAATCGACTAGAAATTGTAGAGAA
ATTTCGTCAAAAAGAAATCACGATTTTGGTAACGACGACAATTTTGGAGCGTGGGGTGACTTTTCCTTGTGTAGATGTTT
TCGTGGTGGAAGCCAACCACCGTCTGTTTAGTCGCAGCGCTCTGGTACAAATTGCTGGACGCGTTGGTCGCAGTATGGAG
CGACCGACAGGCGAGTTAATCTTTTTTCATGACGGTACGACTATGGCGATAGAAAAAGCGATTAAAGAAATTCAGGAGAT
GAATCAGGAGGCCGGTTTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(291-397)

DEAD/DEAH box helicase (DEAD)

(108-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

70.794

98.845

0.7
  comFA/cflA Streptococcus pneumoniae D39

70.794

98.845

0.7
  comFA/cflA Streptococcus pneumoniae R6

70.794

98.845

0.7
  comFA/cflA Streptococcus pneumoniae TIGR4

70.561

98.845

0.697
  comFA/cflA Streptococcus mitis NCTC 12261

70.892

98.383

0.697
  comFA/cflA Streptococcus mitis SK321

70.188

98.383

0.691
  comFA Lactococcus lactis subsp. cremoris KW2

55.276

91.917

0.508
  comFA Latilactobacillus sakei subsp. sakei 23K

40.092

100

0.402
  comFA Bacillus subtilis subsp. subtilis str. 168

40.247

93.533

0.376