Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   PUN35_RS17980 Genome accession   NZ_CP117986
Coordinates   3363999..3365390 (+) Length   463 a.a.
NCBI ID   WP_014481052.1    Uniprot ID   -
Organism   Bacillus sp. N3378-2at     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3358999..3370390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PUN35_RS17955 - 3359158..3360150 (-) 993 WP_014481056.1 LCP family protein -
  PUN35_RS17960 - 3360150..3360803 (-) 654 WP_014481055.1 YigZ family protein -
  PUN35_RS17965 degS 3361020..3362177 (+) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  PUN35_RS17970 degU 3362260..3362949 (+) 690 WP_014481054.1 two-component system response regulator DegU Regulator
  PUN35_RS17975 - 3363047..3363892 (+) 846 WP_014481053.1 DegV family protein -
  PUN35_RS17980 comFA 3363999..3365390 (+) 1392 WP_014481052.1 ATP-dependent helicase ComFA Machinery gene
  PUN35_RS17985 comFB 3365450..3365746 (+) 297 WP_003227989.1 late competence protein ComFB -
  PUN35_RS17990 comFC 3365710..3366432 (+) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  PUN35_RS17995 - 3366505..3366924 (+) 420 WP_014481050.1 TIGR03826 family flagellar region protein -
  PUN35_RS18000 flgM 3367005..3367271 (+) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  PUN35_RS18005 flgN 3367287..3367769 (+) 483 WP_014481048.1 flagellar protein FlgN -
  PUN35_RS18010 flgK 3367788..3369311 (+) 1524 WP_014481047.1 flagellar hook-associated protein FlgK -
  PUN35_RS18015 flgL 3369322..3370218 (+) 897 WP_014481046.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52543.49 Da        Isoelectric Point: 9.7976

>NTDB_id=788930 PUN35_RS17980 WP_014481052.1 3363999..3365390(+) (comFA) [Bacillus sp. N3378-2at]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENEPNWQSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=788930 PUN35_RS17980 WP_014481052.1 3363999..3365390(+) (comFA) [Bacillus sp. N3378-2at]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGA
ACCAAACTGGCAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGGGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCCGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.056

100

0.981