Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGD   Type   Machinery gene
Locus tag   PT966_RS12225 Genome accession   NZ_CP117945
Coordinates   2516369..2516806 (-) Length   145 a.a.
NCBI ID   WP_044053464.1    Uniprot ID   -
Organism   Bacillus velezensis strain ZY1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2511369..2521806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT966_RS12175 (PT966_12175) sinI 2511754..2511927 (+) 174 WP_003153105.1 anti-repressor SinI family protein Regulator
  PT966_RS12180 (PT966_12180) sinR 2511961..2512296 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  PT966_RS12185 (PT966_12185) - 2512344..2513129 (-) 786 WP_003153102.1 TasA family protein -
  PT966_RS12190 (PT966_12190) - 2513193..2513777 (-) 585 WP_003153100.1 signal peptidase I -
  PT966_RS12195 (PT966_12195) tapA 2513749..2514420 (-) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  PT966_RS12200 (PT966_12200) - 2514679..2515008 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  PT966_RS12205 (PT966_12205) - 2515048..2515227 (-) 180 WP_003153093.1 YqzE family protein -
  PT966_RS12210 (PT966_12210) comGG 2515284..2515661 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  PT966_RS12215 (PT966_12215) comGF 2515662..2516057 (-) 396 WP_046559876.1 competence type IV pilus minor pilin ComGF -
  PT966_RS12220 (PT966_12220) comGE 2516071..2516385 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  PT966_RS12225 (PT966_12225) comGD 2516369..2516806 (-) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene
  PT966_RS12230 (PT966_12230) comGC 2516796..2517104 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  PT966_RS12235 (PT966_12235) comGB 2517109..2518146 (-) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  PT966_RS12240 (PT966_12240) comGA 2518133..2519203 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  PT966_RS12245 (PT966_12245) - 2519395..2520345 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  PT966_RS12250 (PT966_12250) - 2520491..2521792 (+) 1302 WP_014305416.1 hemolysin family protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16282.81 Da        Isoelectric Point: 10.3354

>NTDB_id=788427 PT966_RS12225 WP_044053464.1 2516369..2516806(-) (comGD) [Bacillus velezensis strain ZY1]
MNNNRRTENGFTLLESLIVLSLASVLLTVLFTTVPPVCTHLAVRQKTEQLQKDIQLAQETAIAEHKRTKITFLPKEHKYK
LQSGGRIVERSFDSLHITLVTLPESLEFNEKGHPNSGGKIRLKSAGFTYEITVYLGSGNVHAKRK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=788427 PT966_RS12225 WP_044053464.1 2516369..2516806(-) (comGD) [Bacillus velezensis strain ZY1]
TTGAACAATAACAGGCGGACAGAAAACGGGTTTACTCTTCTTGAAAGCCTGATTGTGTTAAGCCTGGCGTCCGTCCTGCT
GACGGTTTTGTTCACGACGGTTCCGCCGGTCTGCACCCACCTTGCCGTGCGGCAAAAAACAGAACAGCTTCAAAAAGATA
TTCAGCTTGCGCAGGAAACGGCTATCGCAGAACATAAAAGAACAAAAATCACTTTTCTTCCAAAAGAGCATAAATACAAA
CTGCAGTCAGGCGGTAGGATTGTCGAGCGTTCTTTTGATTCGCTTCACATTACACTTGTGACTTTACCGGAGAGCCTTGA
ATTTAACGAAAAAGGCCATCCGAATTCGGGAGGAAAGATTCGATTGAAGAGCGCCGGGTTCACCTATGAGATCACAGTTT
ACTTAGGGAGCGGAAATGTCCATGCTAAACGGAAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGD Bacillus subtilis subsp. subtilis str. 168

56.164

100

0.566