Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   PT966_RS12235 Genome accession   NZ_CP117945
Coordinates   2517109..2518146 (-) Length   345 a.a.
NCBI ID   WP_014305414.1    Uniprot ID   -
Organism   Bacillus velezensis strain ZY1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2512109..2523146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT966_RS12185 (PT966_12185) - 2512344..2513129 (-) 786 WP_003153102.1 TasA family protein -
  PT966_RS12190 (PT966_12190) - 2513193..2513777 (-) 585 WP_003153100.1 signal peptidase I -
  PT966_RS12195 (PT966_12195) tapA 2513749..2514420 (-) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  PT966_RS12200 (PT966_12200) - 2514679..2515008 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  PT966_RS12205 (PT966_12205) - 2515048..2515227 (-) 180 WP_003153093.1 YqzE family protein -
  PT966_RS12210 (PT966_12210) comGG 2515284..2515661 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  PT966_RS12215 (PT966_12215) comGF 2515662..2516057 (-) 396 WP_046559876.1 competence type IV pilus minor pilin ComGF -
  PT966_RS12220 (PT966_12220) comGE 2516071..2516385 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  PT966_RS12225 (PT966_12225) comGD 2516369..2516806 (-) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene
  PT966_RS12230 (PT966_12230) comGC 2516796..2517104 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  PT966_RS12235 (PT966_12235) comGB 2517109..2518146 (-) 1038 WP_014305414.1 competence type IV pilus assembly protein ComGB Machinery gene
  PT966_RS12240 (PT966_12240) comGA 2518133..2519203 (-) 1071 WP_003153083.1 competence type IV pilus ATPase ComGA Machinery gene
  PT966_RS12245 (PT966_12245) - 2519395..2520345 (-) 951 WP_003153082.1 magnesium transporter CorA family protein -
  PT966_RS12250 (PT966_12250) - 2520491..2521792 (+) 1302 WP_014305416.1 hemolysin family protein -
  PT966_RS12255 (PT966_12255) - 2521831..2522661 (-) 831 WP_003153080.1 STAS domain-containing protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 39604.88 Da        Isoelectric Point: 9.5658

>NTDB_id=788429 PT966_RS12235 WP_014305414.1 2517109..2518146(-) (comGB) [Bacillus velezensis strain ZY1]
MKRIKRTWPLKDQAAFLKRLGEMTEKGYSLIEGLRLLKLQLHKRQLAELTDGIRRLREGDAFYEVLEALSFHKEAVSICY
FAEKHGELPGAMKQSGDLLQRKLMQTNQIKKMLRYPMFLISSVCVMFYILQSLIIPQFSGIYQSMNMNTSGATAFIFAFF
RHFHEACALALSAAFCLFLYVWFLCKKKSPQDKMLIVVKIPLLGKAAVLFNSYFFSLQLSSLLKSGLSIYDSLTAFKEQS
FLPFYREEAEMLITRLKAGETIESALSGHPYYEKDLAAAVSHGQANGLLHRELYTYSQFMMERLEQNAAKYTGILQPVIY
GVVAGMILIVYMSMLMPMYQMMNQM

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=788429 PT966_RS12235 WP_014305414.1 2517109..2518146(-) (comGB) [Bacillus velezensis strain ZY1]
ATGAAGCGGATTAAACGAACCTGGCCGTTGAAGGATCAAGCGGCTTTTTTGAAAAGGCTCGGTGAAATGACGGAAAAAGG
ATACAGTCTGATTGAAGGGCTCAGACTGCTGAAACTCCAGCTGCATAAACGTCAGCTTGCAGAGCTGACGGACGGAATCC
GCAGGCTGAGGGAAGGTGATGCGTTTTACGAAGTGCTTGAGGCACTGTCTTTTCATAAAGAAGCCGTCTCTATCTGCTAT
TTCGCGGAGAAACACGGAGAATTGCCGGGGGCGATGAAACAAAGCGGCGACTTGTTACAGAGAAAGCTCATGCAGACGAA
TCAAATCAAAAAAATGCTCCGGTATCCGATGTTTTTAATTTCTTCCGTCTGTGTCATGTTTTATATCCTGCAAAGTTTAA
TTATTCCGCAGTTTTCTGGGATTTATCAATCAATGAATATGAATACATCCGGTGCGACTGCTTTCATCTTTGCATTTTTT
CGGCATTTTCATGAAGCCTGCGCACTGGCGCTGTCCGCCGCTTTCTGCTTGTTTTTGTACGTTTGGTTCTTATGTAAGAA
AAAATCCCCGCAAGATAAAATGCTCATTGTTGTGAAAATACCCCTCTTGGGAAAAGCGGCCGTTCTTTTTAACAGCTACT
TTTTCTCTTTACAGCTCAGCAGTCTTCTGAAATCCGGCCTTTCTATCTATGACAGCTTAACAGCTTTTAAAGAACAATCT
TTTCTGCCTTTTTATCGTGAAGAAGCAGAGATGCTCATTACACGTCTGAAAGCCGGAGAAACCATAGAATCCGCTCTTTC
CGGGCATCCGTATTATGAAAAAGATCTTGCGGCTGCAGTCAGCCACGGACAGGCAAACGGCCTGCTTCACCGCGAACTTT
ATACGTACAGCCAGTTTATGATGGAGCGTCTTGAACAAAACGCGGCGAAGTATACCGGCATTCTCCAGCCTGTCATTTAC
GGTGTTGTGGCCGGTATGATTTTAATTGTCTACATGTCAATGCTGATGCCGATGTACCAGATGATGAACCAAATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-337)

Type II secretion system (T2SS), protein F (T2SSF)

(16-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Bacillus subtilis subsp. subtilis str. 168

65.325

93.623

0.612