Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PRJ39_RS21450 Genome accession   NZ_CP117873
Coordinates   5026906..5027964 (-) Length   352 a.a.
NCBI ID   WP_057946404.1    Uniprot ID   A0A1H3RK90
Organism   Lysobacter enzymogenes strain Lj46     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5021906..5032964
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PRJ39_RS21430 (PRJ39_20570) - 5022698..5024740 (-) 2043 WP_091805712.1 type IV pilus secretin PilQ -
  PRJ39_RS21435 (PRJ39_20575) - 5024816..5025367 (-) 552 WP_091805709.1 pilus assembly protein PilP -
  PRJ39_RS21440 (PRJ39_20580) - 5025369..5026052 (-) 684 WP_091805706.1 type 4a pilus biogenesis protein PilO -
  PRJ39_RS21445 (PRJ39_20585) - 5026049..5026906 (-) 858 WP_394538232.1 PilN domain-containing protein -
  PRJ39_RS21450 (PRJ39_20590) pilM 5026906..5027964 (-) 1059 WP_057946404.1 pilus assembly protein PilM Machinery gene
  PRJ39_RS21455 (PRJ39_20595) - 5028576..5031143 (+) 2568 WP_394538233.1 penicillin-binding protein 1A -
  PRJ39_RS21460 - 5031298..5031405 (+) 108 WP_394538234.1 DUF6053 domain-containing protein -
  PRJ39_RS21465 (PRJ39_20600) - 5031553..5032206 (+) 654 WP_091805698.1 hypothetical protein -
  PRJ39_RS21470 - 5032270..5032326 (+) 57 Protein_4242 hypothetical protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 37713.05 Da        Isoelectric Point: 4.6373

>NTDB_id=788162 PRJ39_RS21450 WP_057946404.1 5026906..5027964(-) (pilM) [Lysobacter enzymogenes strain Lj46]
MGLITKSQPALVGVDISSTAVKLLQLSRAGNRYRVEHYAVEPLPPNAVVEKNIVEVEAVGEAIKRAVARSGTRAKHAAAA
VAGSSVITRVIGMPADLDEDDLESQVELEAANYVPYPIDEVNHDFEVLGPMPGSPDMIQVLLAASRSENVEVRASALELG
GLTPRVMDVEAFAIENAFALLADTLSGPRDGLVALVDSGATMTTLNVLRNGRSLYHREQVFGGKQLTDEVMRRYGLSYEE
AGLAKRQGGLPESYQAEVLEPFKEAMVQQVSRLLQFFYAGSEFNRVDQIVLAGGGASIPRIAEMVEEQLGIPTTVANPLA
HMTLGPRVQAHALAQDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=788162 PRJ39_RS21450 WP_057946404.1 5026906..5027964(-) (pilM) [Lysobacter enzymogenes strain Lj46]
GTGGGACTCATCACAAAAAGCCAGCCGGCTCTCGTCGGCGTGGACATCAGTTCGACCGCGGTAAAGTTATTGCAACTTTC
GCGTGCGGGCAATCGCTACCGCGTTGAGCATTACGCGGTCGAGCCGTTGCCGCCGAACGCGGTGGTGGAAAAGAACATCG
TCGAGGTCGAAGCGGTGGGCGAGGCGATCAAACGCGCCGTCGCCCGATCGGGGACCCGCGCCAAGCACGCCGCCGCGGCG
GTCGCCGGTTCCTCGGTGATCACCCGCGTCATCGGCATGCCGGCCGACCTGGACGAAGACGACCTGGAGTCGCAGGTCGA
GCTGGAAGCGGCGAACTACGTGCCGTATCCGATCGACGAGGTCAACCACGATTTCGAAGTCCTCGGCCCGATGCCGGGCA
GCCCGGACATGATCCAGGTGCTGCTGGCCGCGTCGCGTTCGGAGAACGTGGAAGTGCGCGCCTCGGCGCTGGAGCTCGGC
GGCCTGACCCCGCGGGTCATGGACGTGGAAGCCTTCGCGATCGAGAACGCGTTCGCGCTGCTCGCCGACACCCTCAGCGG
GCCGCGCGACGGCCTGGTCGCCCTGGTCGACTCGGGCGCGACCATGACCACGCTGAACGTCCTGCGCAACGGCCGCAGCC
TCTATCACCGCGAGCAGGTGTTCGGCGGCAAGCAGCTGACCGACGAGGTCATGCGCCGCTACGGCCTGAGCTACGAGGAA
GCCGGCCTGGCCAAGCGCCAGGGCGGGCTGCCGGAGAGCTACCAGGCCGAGGTGCTGGAGCCGTTCAAGGAAGCCATGGT
CCAGCAGGTCAGCCGCCTGCTGCAGTTCTTCTATGCCGGCAGCGAGTTCAACCGCGTCGACCAGATCGTCCTGGCCGGCG
GCGGCGCCTCGATCCCGCGCATCGCCGAGATGGTCGAAGAGCAACTCGGCATCCCGACCACGGTGGCCAATCCGCTCGCG
CACATGACCCTGGGGCCGCGCGTGCAGGCGCACGCGCTGGCGCAGGACGCTCCCGCGCTGATGATCGCCTGCGGCCTGGC
CCTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(13-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1H3RK90

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

50.284

100

0.503
  comM Acinetobacter nosocomialis M2

50

100

0.5
  comM Acinetobacter baylyi ADP1

49.716

100

0.497
  pilM Legionella pneumophila strain ERS1305867

43.626

100

0.437