Detailed information NTG0787765

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PT012_RS03510
• Genome accession: NZ_CP117820
• Coordinates: 775675..776805 (-)
• Length: 376 a.a.
• NCBI ID: WP_004084950.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain ICMP 8740
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 770675..781805

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PT012_RS03490 (PT012_03490)	-	770709..771467 (+)	759	WP_027700753.1	LytTR family DNA-binding domain-containing protein	-
PT012_RS03495 (PT012_03495)	hemC	771475..772392 (+)	918	WP_004084955.1	hydroxymethylbilane synthase	-
PT012_RS03500 (PT012_03500)	-	772446..773255 (-)	810	WP_021358437.1	DUF481 domain-containing protein	-
PT012_RS03505 (PT012_03505)	-	774038..775006 (+)	969	WP_004084952.1	YafY family protein	-
PT012_RS03510 (PT012_03510)	pilU	775675..776805 (-)	1131	WP_004084950.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PT012_RS03515 (PT012_03515)	pilT	776960..777994 (-)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
PT012_RS03520 (PT012_03520)	-	778616..778828 (+)	213	WP_021358439.1	hypothetical protein	-
PT012_RS03525 (PT012_03525)	-	779213..780040 (+)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
PT012_RS03530 (PT012_03530)	-	780670..781494 (-)	825	WP_004084943.1	glycosyltransferase family 2 protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42402.63 Da
• Isoelectric Point: 6.3881

>NTDB_id=787765 PT012_RS03510 WP_004084950.1 775675..776805(-) (pilU) [Xylella fastidiosa subsp. multiplex strain ICMP 8740]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHERCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLIDLSLNLKGIIAQQLIPSPDGKERCLAAEVLRDSPLVQDYIRVGEIHKLKEVMQNPNDLGMTTF
DQSLVELYHSGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGVEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=787765 PT012_RS03510 WP_004084950.1 775675..776805(-) (pilU) [Xylella fastidiosa subsp. multiplex strain ICMP 8740]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTGGGTG
CCACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAGATGCATCATCACCCAACGCGAAATAGGTACCGACACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAGGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCATTCTCCGAAACTGGCCATTTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATAGATCTGTCGCTAAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTCCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCCATTAGTAC
AGGACTACATACGCGTAGGAGAGATCCATAAACTCAAAGAAGTCATGCAAAACCCCAACGATCTTGGAATGACGACATTC
GATCAAAGCTTGGTCGAACTCTATCATAGCGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGAAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAGTGGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.853	97.606	0.604
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	51.404	94.681	0.487
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402