Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PT258_RS04885 Genome accession   NZ_CP117814
Coordinates   1081822..1082886 (+) Length   354 a.a.
NCBI ID   WP_136629019.1    Uniprot ID   -
Organism   Legionella pneumophila strain D5241     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1076822..1087886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT258_RS04875 (PT258_04875) - 1077864..1080248 (-) 2385 WP_027226227.1 penicillin-binding protein 1A -
  PT258_RS04880 (PT258_04880) - 1080398..1081405 (-) 1008 WP_013101264.1 hypothetical protein -
  PT258_RS04885 (PT258_04885) pilM 1081822..1082886 (+) 1065 WP_136629019.1 type IV pilus assembly protein PilM Machinery gene
  PT258_RS04890 (PT258_04890) pilN 1082890..1083438 (+) 549 WP_013101266.1 PilN domain-containing protein Machinery gene
  PT258_RS04895 (PT258_04895) pilO 1083452..1084051 (+) 600 WP_013101267.1 type 4a pilus biogenesis protein PilO Machinery gene
  PT258_RS04900 (PT258_04900) pilP 1084048..1084632 (+) 585 WP_013101268.1 pilus assembly protein PilP Machinery gene
  PT258_RS04905 (PT258_04905) pilQ 1084636..1086732 (+) 2097 WP_011946023.1 type IV pilus secretin PilQ Machinery gene
  PT258_RS04910 (PT258_04910) aroK 1087179..1087706 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38787.80 Da        Isoelectric Point: 5.0367

>NTDB_id=787639 PT258_RS04885 WP_136629019.1 1081822..1082886(+) (pilM) [Legionella pneumophila strain D5241]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINNDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=787639 PT258_RS04885 WP_136629019.1 1081822..1082886(+) (pilM) [Legionella pneumophila strain D5241]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAAGTTTACATCTAAAAATGTAGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAACTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGTTTTGTAGACCATATTTTATTGG
CAGGAGGAATGGCCAGGCAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAACCCG
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAATGATGCGCCTACACTAATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

99.718

100

0.997
  comM Acinetobacter nosocomialis M2

42.655

100

0.427
  pilM Acinetobacter baumannii D1279779

42.373

100

0.424
  comM Acinetobacter baylyi ADP1

40.96

100

0.41