Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   OHB58_RS09985 Genome accession   NZ_CP130716
Coordinates   2249157..2250236 (-) Length   359 a.a.
NCBI ID   WP_371651275.1    Uniprot ID   -
Organism   Streptomyces sp. NBC_00150     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2244157..2255236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHB58_RS09965 (OHB58_09920) - 2244965..2245507 (-) 543 WP_371651266.1 adenine phosphoribosyltransferase -
  OHB58_RS09970 (OHB58_09925) secF 2245504..2246610 (-) 1107 WP_371651268.1 protein translocase subunit SecF -
  OHB58_RS09975 (OHB58_09930) secD 2246612..2248369 (-) 1758 WP_371651270.1 protein translocase subunit SecD -
  OHB58_RS09980 (OHB58_09935) yajC 2248514..2248987 (-) 474 WP_371651273.1 preprotein translocase subunit YajC -
  OHB58_RS09985 (OHB58_09940) ruvB 2249157..2250236 (-) 1080 WP_371651275.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  OHB58_RS09990 (OHB58_09945) ruvA 2250262..2250867 (-) 606 WP_371651277.1 Holliday junction branch migration protein RuvA -
  OHB58_RS09995 (OHB58_09950) ruvC 2250864..2251409 (-) 546 WP_371651279.1 crossover junction endodeoxyribonuclease RuvC -
  OHB58_RS10000 (OHB58_09955) - 2251529..2252281 (-) 753 WP_371651281.1 YebC/PmpR family DNA-binding transcriptional regulator -
  OHB58_RS10005 (OHB58_09960) pdxT 2252344..2252931 (-) 588 WP_371651284.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  OHB58_RS10010 (OHB58_09965) pdxS 2252939..2253853 (-) 915 WP_371651286.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  OHB58_RS10015 (OHB58_09970) - 2254057..2254602 (-) 546 WP_371651288.1 hypothetical protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 38257.72 Da        Isoelectric Point: 4.8153

>NTDB_id=787068 OHB58_RS09985 WP_371651275.1 2249157..2250236(-) (ruvB) [Streptomyces sp. NBC_00150]
MNWDETGADATDPRLVDSVADGEDQAVEAALRPKDLDEFVGQEKVREQLDLVLRAARARGATADHVLLSGAPGLGKTTLS
MIIAAEMGAPIRITSGPAIQHAGDLAAILSSLQEGEVLFLDEIHRMSRPAEEMLYMAMEDFRVDVIVGKGPGATAIPLEL
PPFTLVGATTRAGLLPPPLRDRFGFTGHMEFYEPAELERVIHRSARLLDVEIDPAGAAEIAGRSRGTPRIANRLLRRVRD
YAQVKADGVITCEIAAAALRVYEVDSRGLDRLDRAVLQALLKLFGGGPVGLSTLAVAVGEERETVEEVAEPFLVREGLLA
RTPRGRVATPAAWAHLGLVPPQQGPGGVSGSGQQGLFGP

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=787068 OHB58_RS09985 WP_371651275.1 2249157..2250236(-) (ruvB) [Streptomyces sp. NBC_00150]
ATGAACTGGGACGAGACCGGAGCCGACGCCACCGACCCGAGGCTGGTCGACTCGGTGGCCGACGGCGAGGACCAGGCCGT
CGAGGCCGCCCTGCGCCCCAAGGACCTGGACGAGTTCGTCGGCCAGGAGAAGGTCCGCGAGCAGCTCGACCTGGTGCTGC
GCGCGGCCCGCGCGCGCGGCGCCACCGCCGACCACGTGCTGCTCTCCGGCGCCCCCGGCCTCGGCAAGACCACGCTCTCG
ATGATCATCGCGGCCGAGATGGGCGCCCCCATCCGGATCACCAGCGGCCCGGCGATCCAGCACGCGGGCGACCTCGCCGC
GATCCTCTCCTCCCTCCAGGAGGGTGAGGTCCTCTTCCTCGACGAGATCCACCGGATGTCGCGGCCCGCCGAGGAGATGC
TCTACATGGCGATGGAGGACTTCCGGGTCGACGTGATCGTCGGCAAGGGCCCCGGCGCCACCGCCATCCCGCTGGAGCTG
CCCCCCTTCACCCTGGTCGGCGCCACGACGCGCGCGGGGCTGCTGCCGCCCCCGCTGCGCGACCGCTTCGGCTTCACCGG
GCACATGGAGTTCTACGAGCCCGCCGAGCTGGAGCGCGTGATCCACCGCTCGGCCCGCCTCCTCGATGTGGAGATCGACC
CGGCGGGCGCGGCCGAGATCGCCGGCCGCTCGCGCGGCACGCCCCGTATCGCCAACCGGCTGCTGCGCCGCGTCAGGGAC
TATGCGCAGGTCAAGGCCGACGGCGTGATCACCTGCGAGATCGCGGCGGCGGCCCTGCGGGTGTACGAGGTGGACAGCCG
GGGTCTTGACCGGCTGGACCGGGCGGTTTTGCAGGCCTTGCTGAAACTGTTCGGCGGCGGCCCGGTCGGCCTGTCCACGC
TGGCGGTCGCGGTGGGGGAGGAGCGCGAGACCGTCGAAGAGGTGGCCGAGCCCTTCCTCGTACGGGAAGGCCTGCTCGCG
CGGACCCCGCGCGGGCGCGTCGCCACCCCCGCCGCCTGGGCGCACCTCGGCCTCGTCCCGCCCCAGCAGGGCCCGGGCGG
CGTCTCGGGAAGCGGACAACAGGGGCTCTTCGGGCCGTGA

Domains

Predicted by InterProScan.

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(31-189)

RuvB C-terminal winged helix domain (WHD_RuvB)

(267-336)

RuvB AAA lid domain (AAA_lid_4)

(192-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

54.381

92.201

0.501
  ruvB Streptococcus pneumoniae TIGR4

52.778

90.251

0.476
  ruvB Streptococcus pneumoniae R6

52.778

90.251

0.476
  ruvB Streptococcus pneumoniae D39

52.778

90.251

0.476
  ruvB Synechocystis sp. PCC 6803

54.14

87.465

0.474
  ruvB Helicobacter pylori 26695

50.629

88.579

0.448