Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   PSC74_RS00375 Genome accession   NZ_CP117470
Coordinates   69403..70275 (-) Length   290 a.a.
NCBI ID   WP_135318875.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain 516     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 64403..75275
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSC74_RS00350 (PSC74_00350) - 66139..67323 (+) 1185 WP_135318872.1 tetratricopeptide repeat protein -
  PSC74_RS00355 (PSC74_00355) mutT 67332..67745 (+) 414 WP_175044389.1 8-oxo-dGTP diphosphatase MutT -
  PSC74_RS00360 (PSC74_00360) yacG 67806..68000 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  PSC74_RS00365 (PSC74_00365) zapD 68010..68732 (-) 723 WP_024945431.1 cell division protein ZapD -
  PSC74_RS00370 (PSC74_00370) coaE 68770..69384 (-) 615 WP_273775859.1 dephospho-CoA kinase -
  PSC74_RS00375 (PSC74_00375) pilD 69403..70275 (-) 873 WP_135318875.1 prepilin peptidase Machinery gene
  PSC74_RS00380 (PSC74_00380) pilC 70298..71539 (-) 1242 WP_017409439.1 type II secretion system F family protein Machinery gene
  PSC74_RS00385 (PSC74_00385) pilB 71655..73370 (-) 1716 WP_200871048.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  PSC74_RS00390 (PSC74_00390) - 73361..73801 (-) 441 WP_273775860.1 pilin -
  PSC74_RS00395 (PSC74_00395) nadC 74124..74981 (-) 858 WP_135318876.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 290 a.a.        Molecular weight: 32454.74 Da        Isoelectric Point: 6.7410

>NTDB_id=785878 PSC74_RS00375 WP_135318875.1 69403..70275(-) (pilD) [Aeromonas hydrophila strain 516]
MLLITDVFHSLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYLGYFNPETQPQQEERYNLMVPRSACPHCGHA
ITAMENVPLLSWLWLKGRCRECQAPISARYPLVELLTALLSLVVAATFAPGWGLLAALLLTWILVALTFIDLDKMLLPDQ
LTLPLLWGGLLFNLAGGFAPLADAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSSL
VGAFIGIGLILLRNHHQNKPIPFGPYLAIAGWIALLWGDTITRWYLTTFL

Nucleotide


Download         Length: 873 bp        

>NTDB_id=785878 PSC74_RS00375 WP_135318875.1 69403..70275(-) (pilD) [Aeromonas hydrophila strain 516]
ATGTTGCTGATCACTGACGTTTTTCACAGCCTGCCCTGGCTCTATTTCTCGCTGGTATTCCTCTTTTCTCTGATGATCGG
CAGTTTTCTCAACGTGGTCATCCATCGCCTGCCCATCATGCTGGAGCGGGAGTGGCAGGCCGAATATCTCGGCTATTTCA
ATCCGGAAACACAGCCGCAGCAGGAAGAGCGCTACAACCTGATGGTGCCGCGTTCTGCCTGTCCCCATTGTGGCCACGCC
ATCACGGCCATGGAGAACGTCCCCCTGCTCAGCTGGCTCTGGCTCAAGGGGCGCTGCCGTGAATGCCAGGCCCCTATCTC
GGCCCGTTATCCTCTGGTGGAGCTGCTGACGGCCCTGCTCTCGCTGGTCGTCGCCGCGACATTCGCACCGGGCTGGGGCT
TGCTGGCCGCCCTGCTGCTCACCTGGATACTGGTGGCGCTCACTTTCATCGATCTGGACAAGATGCTGCTGCCTGACCAG
CTGACCCTGCCACTGCTGTGGGGCGGCCTGCTGTTCAACCTAGCAGGGGGATTTGCACCGCTGGCAGATGCGGTGATCGG
TGCCATGGCCGGCTATCTGGTACTCTGGAGCCTCTACTGGGCCTTCAAGCTGCTGACCGGCAAGGAAGGGATGGGCTATG
GCGACTTCAAGCTGCTGGCGGCGCTGGGTGCCTGGCTCGGCTGGCAGGCACTGCCCATCGTGTTGCTGCTCTCGTCGCTG
GTTGGCGCTTTCATCGGGATTGGCCTCATCCTGCTGCGCAATCATCATCAGAATAAACCCATCCCCTTCGGCCCCTACCT
GGCCATCGCCGGCTGGATCGCACTGTTGTGGGGCGATACCATCACCCGCTGGTACCTCACGACGTTTCTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-128)

Type IV leader peptidase family (Peptidase_A24)

(138-247)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

60.627

98.966

0.6
  pilD Vibrio campbellii strain DS40M4

55.245

98.621

0.545
  pilD Acinetobacter baumannii D1279779

53.521

97.931

0.524
  pilD Acinetobacter nosocomialis M2

53.521

97.931

0.524
  pilD Neisseria gonorrhoeae MS11

48.352

94.138

0.455