Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSC74_RS00385 Genome accession   NZ_CP117470
Coordinates   71655..73370 (-) Length   571 a.a.
NCBI ID   WP_200871048.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain 516     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 66655..78370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSC74_RS00355 (PSC74_00355) mutT 67332..67745 (+) 414 WP_175044389.1 8-oxo-dGTP diphosphatase MutT -
  PSC74_RS00360 (PSC74_00360) yacG 67806..68000 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  PSC74_RS00365 (PSC74_00365) zapD 68010..68732 (-) 723 WP_024945431.1 cell division protein ZapD -
  PSC74_RS00370 (PSC74_00370) coaE 68770..69384 (-) 615 WP_273775859.1 dephospho-CoA kinase -
  PSC74_RS00375 (PSC74_00375) pilD 69403..70275 (-) 873 WP_135318875.1 prepilin peptidase Machinery gene
  PSC74_RS00380 (PSC74_00380) pilC 70298..71539 (-) 1242 WP_017409439.1 type II secretion system F family protein Machinery gene
  PSC74_RS00385 (PSC74_00385) pilB 71655..73370 (-) 1716 WP_200871048.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  PSC74_RS00390 (PSC74_00390) - 73361..73801 (-) 441 WP_273775860.1 pilin -
  PSC74_RS00395 (PSC74_00395) nadC 74124..74981 (-) 858 WP_135318876.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PSC74_RS00400 (PSC74_00400) - 74987..75457 (-) 471 WP_024944772.1 retropepsin-like aspartic protease family protein -
  PSC74_RS00405 (PSC74_00405) ampD 75605..76177 (+) 573 WP_135318878.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PSC74_RS00410 (PSC74_00410) pdhR 76453..77217 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62910.42 Da        Isoelectric Point: 5.4342

>NTDB_id=785880 PSC74_RS00385 WP_200871048.1 71655..73370(-) (pilB) [Aeromonas hydrophila strain 516]
MPIMTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIP
QKYLNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMENDQDALGMEDIDE
SEISELEVSDEGSRLDESVNTTDDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRF
SARLKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALS
KPQGMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLEL
GFTQQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVT
SLAEINRVTTN

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=785880 PSC74_RS00385 WP_200871048.1 71655..73370(-) (pilB) [Aeromonas hydrophila strain 516]
CTGCCCATAATGACCTCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCTTCTTCCTTGCTCAGTGAGTCCGACTC
ACAGCGCTATCTGAGCCAAGCTAAGGCCCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAAAACGAAATCCTAGACAGCA
AGGCACTGGCAGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCG
CAGAAATACCTCAATCAGAAGCTGATTGAAAAACATCATGTCCTGCCCATCTACACTCAGGGCCACACCCTCTATATCGC
CATGTCGGATCCCACCAATGTGTCGGCGCTGGAGGATTTCGGCTTCAGCTTCGGGTTGCATACCGAAGCGCTGCTGGTAG
AGGAGAACAAACTCACCACAGCCATCGGCAAGCTTATGGAGAACGATCAGGATGCCCTCGGCATGGAGGACATCGACGAG
TCCGAGATCTCCGAACTGGAAGTGTCTGACGAGGGGTCACGACTCGACGAAAGCGTCAATACCACCGACGATGACGCTCC
AATCGTCAAATACATCAATAAAATCATGATGGATGCCATCAAGCGCGGTGCCTCTGACCTTCACTTCGAGCCCTATGAGA
CCAAATACCGCATCCGCTTTCGGATAGACGGGATCTTGCATGAAATAGCTACTCCACCTGTCAATCTGGCTAACCGCTTC
TCGGCCCGCCTCAAGGTGATGGCACGGCTCGACATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAACTGAAGCT
GTCGCGCAACAAGTCGATGGATATGCGGGTCAATACCCTGCCCACGATGTGGGGCGAAAAAATCGTTATCCGTCTGCTCG
ACTCCTCCGCCGCACGCCTCAACATTGAGCAGCTCGGTTTCGACGATCGCCAGAAGGCGCAATATCTGCGTGCCCTCTCG
AAGCCTCAGGGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTTTCGCTCTATACCGGTCTCAATATTCT
CAATACCACCGAGGTCAATATCTCCACCGCTGAAGACCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCA
ATCCCAAGGCAGGACTGACCTTCGCCAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAA
ATCCGGGATCTGGAGACCGCTGAAATTGCTATCAAGGCCGCCCAGACTGGCCACCTGGTGCTATCCACTCTGCACACCAA
CTCGGCGGCGGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCA
TGGCTCAGCGGCTGGCGCGTAAATTGTGCGATCACTGCAAAGCTCCCGAAGTGGTGCCAGAGGCTGAGCTGCTTGAGCTG
GGTTTTACCCAGCAGCAGCTGGCCGCAGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAGGAGTGTTCGGGTGGCTACAA
GGGCCGGGTCGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGC
AAATTGCCGCCATCGCCCAGAAGGAGGGTATGCGTACCTTGCGTACCTCCGGCCTTGAGAAGGCCCGTCTAGGGGTGACC
AGTCTGGCGGAGATTAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.401

100

0.557
  pilB Acinetobacter baylyi ADP1

55.323

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.24

99.124

0.538
  pilB Vibrio cholerae strain A1552

53.982

98.949

0.534
  pilB Vibrio campbellii strain DS40M4

51.943

99.124

0.515
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.424

0.506
  pilF Neisseria gonorrhoeae MS11

46.454

98.774

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

96.848

0.377
  pilF Thermus thermophilus HB27

40.152

92.469

0.371