Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSC74_RS00380 Genome accession   NZ_CP117470
Coordinates   70298..71539 (-) Length   413 a.a.
NCBI ID   WP_017409439.1    Uniprot ID   A0AAX0XY15
Organism   Aeromonas hydrophila strain 516     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 65298..76539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSC74_RS00350 (PSC74_00350) - 66139..67323 (+) 1185 WP_135318872.1 tetratricopeptide repeat protein -
  PSC74_RS00355 (PSC74_00355) mutT 67332..67745 (+) 414 WP_175044389.1 8-oxo-dGTP diphosphatase MutT -
  PSC74_RS00360 (PSC74_00360) yacG 67806..68000 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  PSC74_RS00365 (PSC74_00365) zapD 68010..68732 (-) 723 WP_024945431.1 cell division protein ZapD -
  PSC74_RS00370 (PSC74_00370) coaE 68770..69384 (-) 615 WP_273775859.1 dephospho-CoA kinase -
  PSC74_RS00375 (PSC74_00375) pilD 69403..70275 (-) 873 WP_135318875.1 prepilin peptidase Machinery gene
  PSC74_RS00380 (PSC74_00380) pilC 70298..71539 (-) 1242 WP_017409439.1 type II secretion system F family protein Machinery gene
  PSC74_RS00385 (PSC74_00385) pilB 71655..73370 (-) 1716 WP_200871048.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  PSC74_RS00390 (PSC74_00390) - 73361..73801 (-) 441 WP_273775860.1 pilin -
  PSC74_RS00395 (PSC74_00395) nadC 74124..74981 (-) 858 WP_135318876.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PSC74_RS00400 (PSC74_00400) - 74987..75457 (-) 471 WP_024944772.1 retropepsin-like aspartic protease family protein -
  PSC74_RS00405 (PSC74_00405) ampD 75605..76177 (+) 573 WP_135318878.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45408.55 Da        Isoelectric Point: 9.8637

>NTDB_id=785879 PSC74_RS00380 WP_017409439.1 70298..71539(-) (pilC) [Aeromonas hydrophila strain 516]
MATLTQKQNAPKKVFAFRWSGVNRKGQKVSGELQADSINTVKAELRKQGVNVTKVSKKSQGLFSKGGAKIKPMDIAVVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIAADVETGTPMSEALRRHPRHFDDLYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPTMVILVAIVVTSILLLFVIPQFEDIFKSFGAELPIFTQFVIGISRFMQNWWYVIFGGIA
LAIFLYVRAWRASQKVKDNTDKFVLTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALVSAAGASGNYVYRTAVMAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGSVIH

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=785879 PSC74_RS00380 WP_017409439.1 70298..71539(-) (pilC) [Aeromonas hydrophila strain 516]
ATGGCAACGCTAACCCAGAAACAGAATGCCCCCAAGAAAGTCTTCGCCTTTCGCTGGAGCGGGGTGAATCGCAAGGGCCA
GAAGGTCTCCGGCGAATTGCAGGCCGACAGCATCAACACGGTCAAGGCAGAGCTGCGCAAGCAAGGTGTCAACGTCACCA
AGGTGAGCAAGAAGAGCCAGGGGCTCTTCTCCAAGGGCGGCGCCAAGATCAAGCCGATGGACATCGCCGTCGTCTCGCGC
CAGATCACCACCATGCTGTCAGCCGGCGTACCTCTGGTCCAGAGCCTGCAGATCATCGCTCGCAGCCACGAGAAGGCGGC
CATGCGCGAGCTCATGGGCCAGATTGCTGCCGATGTGGAGACCGGCACGCCTATGTCCGAAGCGCTGCGCCGCCATCCCC
GCCACTTTGACGATCTCTACTGCGATCTAGTGGAGGCTGGCGAGCAGTCCGGCGCCCTGGAGACCATCTACGATCGCATC
GCCACCTATCGCGAGAAATCAGAAGCCCTCAAGTCCAAGATCAAGAAAGCCATGTTCTACCCCACCATGGTCATATTGGT
GGCCATTGTCGTTACCTCCATCCTGCTGCTGTTCGTCATTCCCCAGTTCGAGGACATCTTCAAGAGTTTCGGCGCCGAGC
TGCCCATCTTCACCCAGTTCGTCATCGGCATCTCCCGTTTCATGCAGAACTGGTGGTACGTGATCTTTGGCGGGATAGCG
CTCGCCATCTTTCTCTACGTGCGCGCCTGGCGCGCCTCCCAGAAGGTCAAGGACAACACCGACAAGTTCGTACTGACCAT
CCCCGTAGTCGGCATGATATTGCACAAGGCCGCCATGGCCCGCTTCGCCCGCACCCTCTCCACTACCTTTTCCGCCGGTA
TCCCGCTGGTGGATGCCTTGGTCTCTGCGGCAGGTGCCTCCGGCAACTATGTCTATCGCACAGCCGTCATGGCCATTCGC
AACGAGGTGGTGGCCGGCATGCAGATCAACGTGGCCATGCGCACCGTCGATCTGTTCCCGGACATGGTGATCCAGATGGT
GATGATCGGCGAGGAGTCTGGCGCCATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAGCAGGAGGTGGACGATC
TGGTCGACGGCCTCACCAGCCTGCTGGAGCCTCTCATCATGGTGGTGCTGGGGGTGCTGGTCGGCGGCATGGTGGTCGCC
ATGTATCTGCCCATCTTCAAACTGGGATCGGTGATCCACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

57.323

95.884

0.55
  pilC Acinetobacter baumannii D1279779

54

96.852

0.523
  pilC Acinetobacter baylyi ADP1

52.393

96.126

0.504
  pilC Legionella pneumophila strain ERS1305867

51.358

98.063

0.504
  pilC Vibrio cholerae strain A1552

47.739

96.368

0.46
  pilC Vibrio campbellii strain DS40M4

46.348

96.126

0.446
  pilG Neisseria gonorrhoeae MS11

40.247

98.063

0.395
  pilG Neisseria meningitidis 44/76-A

40

98.063

0.392
  pilC Thermus thermophilus HB27

37.75

96.852

0.366