Detailed information    

experimental Experimentally validated

Overview

Name   recS   Type   Machinery gene
Locus tag   BSU_23020 Genome accession   NC_000964
Coordinates   2406922..2408412 (-) Length   496 a.a.
NCBI ID   NP_390183.1    Uniprot ID   P50729
Organism   Bacillus subtilis subsp. subtilis str. 168     
Function   homologous recombination   
Homologous recombination

Function

The recU and recS proteins are required for DNA repair and intramolecular recombination.


Genomic Context

Location: 2401922..2413412
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSU_22960 (BSU22960) gudB 2402067..2403350 (-) 1284 NP_390177.2 cryptic glutamate dehydrogenase (active after removal of a 9 bp insert) -
  BSU_22970 (BSU22970) mecB 2403506..2404090 (-) 585 NP_390178.1 adaptor to ClpC; regulator of competence and sporulation -
  BSU_22980 (BSU22980) ypbG 2404249..2405028 (-) 780 NP_390179.1 putative phosphoesterase -
  BSU_22990 (BSU22990) ypbF 2405114..2405557 (-) 444 NP_390180.1 hypothetical protein -
  BSU_23000 (BSU23000) ypbE 2405620..2406342 (-) 723 NP_390181.1 putative enzyme possibly involved in cell wall turnover -
  BSU_23010 (BSU23010) ypbD 2406293..2406862 (-) 570 NP_390182.1 conserved membrane protein of unknown function -
  BSU_23020 (BSU23020) recS 2406922..2408412 (-) 1491 NP_390183.1 ATP-dependent DNA helicase Machinery gene
  BSU_23030 (BSU23030) gmmB 2408405..2409463 (-) 1059 NP_390184.1 subunit of the platform for genome maintenance machinery -
  BSU_23040 (BSU23040) fer 2409729..2409977 (+) 249 NP_390185.1 ferredoxin -
  BSU_23050 (BSU23050) fmnP 2410017..2410589 (-) 573 NP_390186.1 FMN permease -
  BSU_23070 (BSU23070) serA 2411086..2412663 (+) 1578 NP_390188.2 3-phosphoglycerate dehydrogenase -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 56508.11 Da        Isoelectric Point: 6.6626

>NTDB_id=637 BSU_23020 NP_390183.1 2406922..2408412(-) (recS) [Bacillus subtilis subsp. subtilis str. 168]
MTKLQQTLYQFFGFTSFKKGQQDIIESILSGKDTIAMLPTGGGKSLCYQLPGYMLDGMVLIVSPLLSLMEDQVQQLKARG
EKRAAALNSMLNRQERQFVLEHIHRYKFLYLSPEALQSPYVLEKLKSVPISLFVIDEAHCISEWGHDFRPDYSKLGQLRK
KLGHPPVLALTATATKETLQDVMNLLELQHAVRHLNSVNRPNIALRVENAADTAEKIDRVIQLVENLQGPGIVYCPTRKW
AKELAGEIKSKTSSRADFYHGGLESGDRILIQQQFIHNQLDVICCTNAFGMGVDKPDIRYVIHFHLPQTAEAFMQEIGRA
GRDGKPSVSILLRAPGDFELQEQIIQMESVTAEEIADVIRVLEKTEERDERRLRDVLLQYGVGETQARMMIHLFMQGKTS
VELMKKEISYRMELKLEKMHRVSFLLQRDGCLRQALLTYFDESYEPDDGNLPCCSHCGFDLSLYEQKGERSKMAPLDSWS
SELHRIFSLQTVGELN

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=637 BSU_23020 NP_390183.1 2406922..2408412(-) (recS) [Bacillus subtilis subsp. subtilis str. 168]
ATGACTAAATTACAGCAAACGTTATATCAGTTTTTTGGTTTTACTTCCTTTAAAAAAGGGCAGCAGGACATTATTGAAAG
CATACTCAGCGGGAAGGATACCATTGCAATGCTCCCGACTGGGGGAGGGAAATCGCTTTGCTACCAGCTGCCTGGTTATA
TGCTTGATGGCATGGTATTAATCGTTTCTCCACTGCTTTCTTTAATGGAGGATCAGGTGCAGCAGCTCAAAGCCCGGGGG
GAAAAGCGTGCTGCAGCTTTGAACAGCATGCTTAACAGGCAGGAAAGACAATTTGTTTTAGAACATATTCATCGATATAA
ATTTTTGTACTTGTCTCCTGAAGCTTTACAGTCTCCATATGTATTGGAAAAATTGAAAAGCGTTCCGATTAGCCTATTTG
TTATTGACGAAGCGCATTGTATTTCTGAATGGGGACACGACTTCAGGCCTGATTATTCAAAGCTCGGACAGCTGAGAAAA
AAACTTGGACACCCACCCGTTCTGGCATTGACCGCCACGGCTACAAAGGAAACGCTGCAAGATGTCATGAACCTGCTGGA
GCTGCAGCATGCTGTACGCCATCTCAACTCAGTCAACCGCCCAAACATCGCACTGAGGGTTGAAAATGCGGCGGATACTG
CCGAAAAAATAGATAGGGTCATTCAGCTGGTTGAAAACCTGCAAGGTCCGGGAATTGTTTATTGTCCAACACGGAAATGG
GCTAAGGAATTAGCGGGTGAAATTAAAAGCAAAACGAGCAGCAGAGCCGACTTTTACCATGGCGGTTTGGAGTCAGGGGA
CAGGATTTTAATTCAGCAGCAGTTTATTCATAACCAGCTTGATGTGATATGTTGTACGAATGCGTTTGGAATGGGTGTCG
ATAAACCTGATATCAGATATGTGATTCATTTTCATCTCCCGCAGACGGCAGAAGCTTTTATGCAGGAAATCGGCAGAGCG
GGACGCGACGGAAAGCCGAGTGTCAGCATTTTGCTGCGGGCGCCAGGGGATTTTGAATTGCAGGAACAAATTATCCAGAT
GGAGAGTGTCACAGCTGAAGAAATCGCCGACGTAATCCGTGTGCTAGAGAAGACGGAGGAGCGGGATGAGAGAAGGCTGC
GTGACGTATTACTTCAGTATGGCGTCGGAGAGACACAGGCCCGTATGATGATTCATCTCTTTATGCAGGGAAAAACATCA
GTTGAACTGATGAAGAAAGAAATTTCATATCGCATGGAGCTGAAGCTGGAAAAAATGCACCGTGTATCATTTTTGCTTCA
GCGTGACGGCTGTTTAAGGCAGGCACTGTTAACCTACTTTGATGAATCATACGAGCCTGATGACGGAAACCTCCCGTGCT
GTTCACATTGCGGTTTTGATTTATCCTTGTATGAACAAAAAGGGGAACGGAGTAAAATGGCGCCATTAGACAGCTGGAGT
TCGGAATTGCACCGGATATTCAGCTTGCAGACTGTAGGTGAGCTGAACTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(20-180)

Helicase conserved C-terminal domain (Helicase_C)

(215-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P50729

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] S Fernández et al. (1998) Genetic recombination in Bacillus subtilis 168: effects of recU and recS mutations on DNA repair and homologous recombination. Journal of Bacteriology 180(13):3405-9. [PMID: 9642195]