Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   LLL8_RS11435 Genome accession   NZ_AP025700
Coordinates   2287801..2288820 (-) Length   339 a.a.
NCBI ID   WP_047206974.1    Uniprot ID   -
Organism   Lactococcus lactis strain L8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2282801..2293820
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLL8_RS11400 (LLL8_22340) - 2282955..2283203 (-) 249 WP_251899967.1 helix-turn-helix transcriptional regulator -
  LLL8_RS11405 (LLL8_22350) - 2283384..2284043 (+) 660 WP_251899968.1 hypothetical protein -
  LLL8_RS11410 (LLL8_22360) - 2284306..2284860 (+) 555 WP_251899969.1 helix-turn-helix domain-containing protein -
  LLL8_RS11415 (LLL8_22370) - 2284871..2285458 (+) 588 WP_251899970.1 hypothetical protein -
  LLL8_RS11420 (LLL8_22380) - 2285520..2286071 (+) 552 WP_251899971.1 hypothetical protein -
  LLL8_RS11425 (LLL8_22390) - 2286193..2287650 (+) 1458 WP_228248619.1 recombinase family protein -
  LLL8_RS11430 - 2287647..2287787 (-) 141 WP_247649129.1 hypothetical protein -
  LLL8_RS11435 (LLL8_22400) comGB 2287801..2288820 (-) 1020 WP_047206974.1 competence type IV pilus assembly protein ComGB Machinery gene
  LLL8_RS11440 (LLL8_22410) comGA 2288768..2289706 (-) 939 WP_058206479.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 339 a.a.        Molecular weight: 38256.98 Da        Isoelectric Point: 10.1935

>NTDB_id=78577 LLL8_RS11435 WP_047206974.1 2287801..2288820(-) (comGB) [Lactococcus lactis strain L8]
MDISHLLKPKGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLASGQALSELLESLSFSKNV
VTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQLNSGSNFATLLINHLPLV
FFSFGAFIFLLTALSVTLLKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVELRQIINLMKKQKSRIFSE
VGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKIDRLMQLIQPLVFIFVALMI
ILLYAAMLLPIYSNMGSGI

Nucleotide


Download         Length: 1020 bp        

>NTDB_id=78577 LLL8_RS11435 WP_047206974.1 2287801..2288820(-) (comGB) [Lactococcus lactis strain L8]
ATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATCCAACTGATGGG
AAATCTTTTGAATAGTGGATTTAATTTAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTA
CATTAAAAATGAAAGAAGGTCTCGCTTCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTG
GTGACACAACTTGCTTTGGTAGAAGTGCATGGTAACTTGTCAGGGACGATGCAATTAGTTGAACTTCATCTAAAAAAACA
GCTTAAAGTTAAAAATAAATTAGTGGAAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGG
GCTTAAAAAATTATTTACTACCACAACTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTA
TTTTTTTCGTTTGGAGCTTTTATATTTTTACTGACAGCTTTGTCAGTAACTCTTTTAAAACGAAAATCAGCAATAATGAA
TTTTACATTTCTTGTTAAATTACCTCTGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGG
GAAATCTAATTGCTCAAGGTGTTGAATTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAA
GTTGGCAAGAATCTTGATTTAGAATTGAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACC
CGAGCTCTCGTTAATGATTGAATATGGAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTGTATGCGGATGAATGTT
GGGAACAATTTTTTACAAAGATAGATCGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTTGTAGCATTGATGATT
ATTTTGCTATATGCAGCCATGTTACTCCCCATCTATTCAAATATGGGTTCTGGAATATAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein F (T2SSF)

(23-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-330)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Lactococcus lactis subsp. cremoris KW2

83.728

99.705

0.835
  comGB Streptococcus gordonii str. Challis substr. CH1

53.593

98.525

0.528
  comGB/cglB Streptococcus mitis SK321

51.343

98.82

0.507
  comGB/cglB Streptococcus pneumoniae TIGR4

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae Rx1

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae D39

51.045

98.82

0.504
  comGB/cglB Streptococcus pneumoniae R6

51.045

98.82

0.504
  comGB/cglB Streptococcus mitis NCTC 12261

50.448

98.82

0.499
  comGB Streptococcus mutans UA140

52.866

92.625

0.49
  comGB Streptococcus mutans UA159

52.548

92.625

0.487

Multiple sequence alignment