Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PSH86_RS02325 Genome accession   NZ_CP117440
Coordinates   544018..545082 (+) Length   354 a.a.
NCBI ID   WP_109752037.1    Uniprot ID   -
Organism   Pseudomonas sp. FP2300     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 539018..550082
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSH86_RS02310 (PSH86_02310) rpmE 539275..539499 (+) 225 WP_003196965.1 50S ribosomal protein L31 -
  PSH86_RS02315 (PSH86_02315) - 539803..541071 (+) 1269 WP_109752035.1 malic enzyme-like NAD(P)-binding protein -
  PSH86_RS02320 (PSH86_02320) - 541366..543810 (-) 2445 WP_178082464.1 penicillin-binding protein 1A -
  PSH86_RS02325 (PSH86_02325) comM 544018..545082 (+) 1065 WP_109752037.1 pilus assembly protein PilM Machinery gene
  PSH86_RS02330 (PSH86_02330) - 545082..545648 (+) 567 WP_305487495.1 PilN domain-containing protein -
  PSH86_RS02335 (PSH86_02335) pilO 545645..546268 (+) 624 WP_109752039.1 type 4a pilus biogenesis protein PilO -
  PSH86_RS02340 (PSH86_02340) - 546265..546792 (+) 528 WP_109752040.1 pilus assembly protein PilP -
  PSH86_RS02345 (PSH86_02345) pilQ 546806..548893 (+) 2088 WP_116833986.1 type IV pilus secretin PilQ Machinery gene
  PSH86_RS02350 (PSH86_02350) aroK 548898..549416 (+) 519 WP_003196983.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38067.64 Da        Isoelectric Point: 4.9070

>NTDB_id=785149 PSH86_RS02325 WP_109752037.1 544018..545082(+) (comM) [Pseudomonas sp. FP2300]
MLRLFNKKAHTLLGIDISSTSVKLLELSRQGERYRVESYAVEPLPANAVVEKNIADLEGVGQALSRLLAKAKTPVRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVVGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=785149 PSH86_RS02325 WP_109752037.1 544018..545082(+) (comM) [Pseudomonas sp. FP2300]
GTGCTACGACTCTTCAATAAAAAAGCCCATACGCTTCTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTCGAGTT
GAGCCGCCAGGGCGAGCGATATCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCGGCCAACGCCGTGGTCGAGAAGAACA
TCGCCGATCTCGAAGGGGTGGGCCAGGCATTGTCACGGCTGTTGGCCAAGGCCAAGACGCCCGTGCGTAGCGTGGCGGTG
GCCGTTGCGGGGTCGGCGGTGATTACCAAGACCATCGAGATGGACGCCGGGTTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGATGAGGTGGCCATCGATTTCGAAGTGGTGGGCGTTTCGCCGC
GCAGTGCCGAGCGCGTCGAAGTGCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCGTTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGACGTGGAGGCTTATGCGCTGGAGCGTGCTTTCGGCTTGCTCGCCACGCAACTGGC
CGCCTCCCAGGAACGGCTGACTGTGGCGGTTATCGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGCCGCCAACTGACCGAGGAAATTCAGCGCCGCTATGGCTTGACGCCC
GAGCAGGCCGGCTTGGCAAAGAAGCAGGGAGGGCTGCCGGACGATTACCTGAGCGAAGTGTTGCAACCCTTTCGCGAGGC
TCTGGTACAGCAGGTCTCGCGATCCCTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTCGACCACATCCTGCTGG
CCGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAACAACGCCTGGGTACCCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGGGCAGCAAGGTCAATGCCGGGGCGCTGGCCAGTGATGCACCGGCGCTGATGATTGCCTGCGG
GCTGGCCCTCAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483