NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	NL415_RS12790	Genome accession	NZ_CP117016
Coordinates	2453248..2454372 (-)	Length	374 a.a.
NCBI ID	WP_000228562.1	Uniprot ID	-
Organism	Escherichia coli strain MLI121
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 2448248..2459372

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NL415_RS12760 (NL415_012760)	yrdA	2450048..2450602 (+)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-
NL415_RS12765 (NL415_012765)	yrdB	2450578..2450835 (-)	258	WP_001070567.1	DUF1488 domain-containing protein	-
NL415_RS12770 (NL415_012770)	aroE	2450832..2451650 (-)	819	WP_000451231.1	shikimate dehydrogenase	-
NL415_RS12775 (NL415_012775)	tsaC	2451655..2452227 (-)	573	WP_001366671.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
NL415_RS12780 (NL415_012780)	yrdD	2452232..2452774 (-)	543	WP_001129723.1	type I DNA topoisomerase	-
NL415_RS12785 (NL415_012785)	smg	2452803..2453276 (-)	474	WP_000460672.1	DUF494 family protein Smg	-
NL415_RS12790 (NL415_012790)	dprA	2453248..2454372 (-)	1125	WP_000228562.1	DNA-protecting protein DprA	Machinery gene
NL415_RS12795 (NL415_012795)	def	2454502..2455011 (+)	510	WP_000114984.1	peptide deformylase	-
NL415_RS12800 (NL415_012800)	fmt	2455026..2455973 (+)	948	WP_000004443.1	methionyl-tRNA formyltransferase	-
NL415_RS12805 (NL415_012805)	rsmB	2456019..2457308 (+)	1290	WP_001469469.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
NL415_RS12810 (NL415_012810)	trkA	2457330..2458706 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
NL415_RS12815 (NL415_012815)	mscL	2458836..2459246 (+)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40975.98 Da Isoelectric Point: 6.4377

                                >NTDB_id=782022 NL415_RS12790 WP_000228562.1 2453248..2454372(-) (dprA) [Escherichia coli strain MLI121]

MVDTEIWLRLMSISSLYGDNMVRIAHWLAKQSYIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVAGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=782022 NL415_RS12790 WP_000228562.1 2453248..2454372(-) (dprA) [Escherichia coli strain MLI121]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTACGGCGATAATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGTATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGCAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCGCGTCGCCATGCCCGACTGGCTACCAGTTTGCT

TGAACATGGTGGGGCTCTTGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTTTAAGTAAAGGTGTACTAGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGACCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterproScan.

DprA winged helix domain (DprA_WH)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae strain A1552	48.71	82.888	0.404
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	43.917	90.107	0.396
dprA	Legionella pneumophila strain ERS1305867	44.377	87.968	0.39

Detailed information NTG0782022